PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS10105 [old locus tag: SACOL1932 ]
pan locus tag^?: SAUPAN004857000
symbol: SACOL_RS10105
pan gene symbol^?: sgtB
• synonym:
• product: monofunctional glycosyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS10105 [old locus tag: SACOL1932 ]
• symbol: SACOL_RS10105
• product: monofunctional glycosyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1996766..1997575
• length: 810
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1996766..1997575) NCBI
• BioCyc: SACOL_RS10105 BioCyc
• MicrobesOnline: see SACOL1932

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAGAAGCGATAGGTACTCAAACTCAAATGAACATTTTGAGCATATGAAACACGAA
  CCTCACTATAATACGTATTATCAACCAGTTGGCAAACCGCCGAAAAAGAAAAAAAGTAAA
  CGAATACTATTAAAAATATTATTAACCATTCTAATCATTATCGCATTGTTTATTGGTATC
  ATGTATTTTTTATCTACACGCGATAATGTGGATGAACTAAGAAAAATTGAAAATAAAAGT
  AGTTTTGTGTCAGCTGATAACATGCCAGAGTATGTTAAAGGTGCCTTTATTTCAATGGAA
  GATGAACGATTCTACAATCATCATGGATTCGATTTGAAAGGTACAACTAGAGCTTTATTT
  TCAACGATTAGCGACAGAGATGTGCAAGGTGGTAGTACCATTACACAACAAGTTGTCAAA
  AATTATTTTTATGATAATGATCGTTCATTTACTAGAAAAGTAAAAGAATTATTTGTAGCT
  CATCGAGTTGAAAAACAATATAATAAGAACGAAATTTTAAGCTTTTATTTAAATAATATT
  TACTTTGGGGATAATCAATATACGCTTGAGGGCGCAGCAAACCATTACTTTGGAACAACC
  GTGAATAAAAATAGTACAACAATGTCTCACATAACAGTTTTACAAAGCGCTATTTTAGCT
  AGTAAAGTCAATGCACCTAGCGTATATAATATCAATAATATGTCAGAGAATTTTACGCAA
  CGTGTAAGCACGAACTTAGAAAAAATGAAGCAACAAAATTATATCAATGAAACACAATAT
  CAACAGGCTATGTCACAATTAAATCGTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS10105 [old locus tag: SACOL1932 ]
• symbol: SACOL_RS10105
• description: monofunctional glycosyltransferase
• length: 269
• theoretical pI: 9.82726
• theoretical MW: 31459.4
• GRAVY: -0.664312

⊟Function

• ⊞reaction:
  EC 2.4.1.129?  ExPASy
  Peptidoglycan glycosyltransferase (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
• ⊞TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 199.4)
  and 5 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1B (TIGR02071; HMM-score: 131.8)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan monofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 127.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 114.3)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides oligosaccharide repeat unit polymerase (TIGR04370; HMM-score: 14.2)
  YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 13.3)
• TheSEED: see SACOL1932
• ⊞PFAM:
  Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 192.4)
  and 5 more
  no clan defined Orf78; Orf78 (ac78) (PF06024; HMM-score: 18.5)
  Glyoxalase (CL0104) Teneurin_ABD; Teneurin antibiotic-binding-like domain (PF23538; HMM-score: 13)
  no clan defined AmoC; Ammonia monooxygenase/methane monooxygenase, subunit C (PF04896; HMM-score: 12.2)
  BAAB_hairpin (CL0834) DUF898; Bacterial protein of unknown function (DUF898) (PF05987; HMM-score: 11.7)
  E-set (CL0159) DUF4179; Domain of unknown function (DUF4179) (PF13786; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helix: 1
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.8095
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.1904
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001771
  • TAT(Tat/SPI): 0.000242
  • LIPO(Sec/SPII): 0.003023
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446753124 NCBI
• RefSeq: WP_000830380 NCBI
• UniProt: see SACOL1932

⊟Protein sequence

• MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILLKILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDERFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVAHRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAILASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR

⊟Experimental data

• experimentally validated: see SACOL1932
• protein localization: see SACOL1932
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications