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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10105 [old locus tag: SACOL1932 ]
  • pan locus tag?: SAUPAN004857000
  • symbol: SACOL_RS10105
  • pan gene symbol?: sgtB
  • synonym:
  • product: monofunctional glycosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS10105 [old locus tag: SACOL1932 ]
  • symbol: SACOL_RS10105
  • product: monofunctional glycosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1996766..1997575
  • length: 810
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1996766..1997575) NCBI
  • BioCyc: SACOL_RS10105 BioCyc
  • MicrobesOnline: see SACOL1932

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAAGAAGCGATAGGTACTCAAACTCAAATGAACATTTTGAGCATATGAAACACGAA
    CCTCACTATAATACGTATTATCAACCAGTTGGCAAACCGCCGAAAAAGAAAAAAAGTAAA
    CGAATACTATTAAAAATATTATTAACCATTCTAATCATTATCGCATTGTTTATTGGTATC
    ATGTATTTTTTATCTACACGCGATAATGTGGATGAACTAAGAAAAATTGAAAATAAAAGT
    AGTTTTGTGTCAGCTGATAACATGCCAGAGTATGTTAAAGGTGCCTTTATTTCAATGGAA
    GATGAACGATTCTACAATCATCATGGATTCGATTTGAAAGGTACAACTAGAGCTTTATTT
    TCAACGATTAGCGACAGAGATGTGCAAGGTGGTAGTACCATTACACAACAAGTTGTCAAA
    AATTATTTTTATGATAATGATCGTTCATTTACTAGAAAAGTAAAAGAATTATTTGTAGCT
    CATCGAGTTGAAAAACAATATAATAAGAACGAAATTTTAAGCTTTTATTTAAATAATATT
    TACTTTGGGGATAATCAATATACGCTTGAGGGCGCAGCAAACCATTACTTTGGAACAACC
    GTGAATAAAAATAGTACAACAATGTCTCACATAACAGTTTTACAAAGCGCTATTTTAGCT
    AGTAAAGTCAATGCACCTAGCGTATATAATATCAATAATATGTCAGAGAATTTTACGCAA
    CGTGTAAGCACGAACTTAGAAAAAATGAAGCAACAAAATTATATCAATGAAACACAATAT
    CAACAGGCTATGTCACAATTAAATCGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    810

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS10105 [old locus tag: SACOL1932 ]
  • symbol: SACOL_RS10105
  • description: monofunctional glycosyltransferase
  • length: 269
  • theoretical pI: 9.82726
  • theoretical MW: 31459.4
  • GRAVY: -0.664312

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.129?  ExPASy
    Peptidoglycan glycosyltransferase (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 199.4)
    and 5 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1B (TIGR02071; HMM-score: 131.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan monofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 127.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 114.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides oligosaccharide repeat unit polymerase (TIGR04370; HMM-score: 14.2)
    YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 13.3)
  • TheSEED: see SACOL1932
  • PFAM:
    Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 186.5)
    and 2 more
    no clan defined Orf78; Orf78 (ac78) (PF06024; HMM-score: 18.6)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001771
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.003023
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILLKILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDERFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVAHRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAILASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1932
  • protein localization: see SACOL1932
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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