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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02012
  • pan locus tag?: SAUPAN004857000
  • symbol: SAOUHSC_02012
  • pan gene symbol?: sgtB
  • synonym:
  • product: glycosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02012
  • symbol: SAOUHSC_02012
  • product: glycosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1921172..1921981
  • length: 810
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAAGAAGCGATAGGTACTCAAACTCAAATGAACATTTTGAGCATATGAAACACGAA
    CCTCACTATAATACGTATTATCAACCAGTTGGCAAACCGCCGAAAAAGAAAAAAAGTAAA
    CGAATACTATTAAAAATATTATTAACCATTCTAATCATTATCGCATTGTTTATTGGTATC
    ATGTATTTTTTATCTACACGCGATAATGTGGATGAACTAAGAAAAATTGAAAATAAAAGT
    AGTTTTGTGTCAGCTGATAACATGCCAGAGTATGTTAAAGGTGCCTTTATTTCAATGGAA
    GATGAACGATTCTACAATCATCATGGATTCGATTTGAAAGGTACAACTAGAGCTTTATTT
    TCAACGATTAGCGACAGAGATGTGCAAGGTGGTAGTACCATTACACAACAAGTTGTCAAA
    AATTATTTTTATGATAATGATCGTTCATTTACTAGAAAAGTAAAAGAATTATTTGTAGCT
    CATCGAGTTGAAAAACAATATAATAAGAACGAAATTTTAAGCTTTTATTTAAATAATATT
    TACTTTGGGGATAATCAATATACGCTTGAGGGCGCAGCAAACCATTACTTTGGAACAACC
    GTGAATAAAAATAGTACAACAATGTCTCACATAACAGTTTTACAAAGCGCTATTTTAGCT
    AGTAAAGTCAATGCACCTAGCGTATATAATATCAATAATATGTCAGAGAATTTTACGCAA
    CGTGTAAGCACGAACTTAGAAAAAATGAAGCAACAAAATTATATCAATGAAACACAATAT
    CAACAGGCTATGTCACAATTAAATCGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    810

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02012
  • symbol: SAOUHSC_02012
  • description: glycosyltransferase
  • length: 269
  • theoretical pI: 9.82726
  • theoretical MW: 31459.4
  • GRAVY: -0.664312

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.129?  ExPASy
    Peptidoglycan glycosyltransferase (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 199.4)
    and 5 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1B (TIGR02071; HMM-score: 131.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan monofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 127.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 114.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides oligosaccharide repeat unit polymerase (TIGR04370; HMM-score: 14.2)
    YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 13.3)
  • TheSEED  :
    • Monofunctional biosynthetic peptidoglycan transglycosylase
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-)
  • PFAM:
    Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 186.5)
    and 2 more
    no clan defined Orf78; Orf78 (ac78) (PF06024; HMM-score: 18.6)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001771
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.003023
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILLKILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDERFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVAHRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAILASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Q M Wang, R B Peery, R B Johnson, W E Alborn, W K Yeh, P L Skatrud
Identification and characterization of a monofunctional glycosyltransferase from Staphylococcus aureus.
J Bacteriol: 2001, 183(16);4779-85
[PubMed:11466281] [WorldCat.org] [DOI] (P p)S A Borchardt, A V Babwah, R K Jayaswal
Sequence analysis of the region downstream from a peptidoglycan hydrolase-encoding gene from Staphylococcus aureus NCTC8325.
Gene: 1993, 137(2);253-8
[PubMed:7905453] [WorldCat.org] [DOI] (P p)

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