PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1766 [new locus tag: NWMN_RS10100 ]
• pan locus tag^?: SAUPAN004857000
• symbol: NWMN_1766
• pan gene symbol^?: sgtB
• synonym:
• product: glycosyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1766 [new locus tag: NWMN_RS10100 ]
• symbol: NWMN_1766
• product: glycosyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1977903..1978712
• length: 810
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331053 NCBI
• RefSeq: YP_001332800 NCBI
• BioCyc:
• MicrobesOnline: 3707354 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAGAAGCGATAGGTACTCAAACTCAAATGAACATTTTGAGCATATGAAACACGAA
  CCTCACTATAATACGTATTATCAACCAGTTGGCAAACCGCCGAAAAAGAAAAAAAGTAAA
  CGAATACTATTAAAAATATTATTAACCATTCTAATCATTATCGCATTGTTTATTGGTATC
  ATGTATTTTTTATCTACACGCGATAATGTGGATGAACTAAGAAAAATTGAAAATAAAAGT
  AGTTTTGTGTCAGCTGATAACATGCCAGAGTATGTTAAAGGTGCCTTTATTTCAATGGAA
  GATGAACGATTCTACAATCATCATGGATTCGATTTGAAAGGTACAACTAGAGCTTTATTT
  TCAACGATTAGCGACAGAGATGTGCAAGGTGGTAGTACCATTACACAACAAGTTGTCAAA
  AATTATTTTTATGATAATGATCGTTCATTTACTAGAAAAGTAAAAGAATTATTTGTAGCT
  CATCGAGTTGAAAAACAATATAATAAGAACGAAATTTTAAGCTTTTATTTAAATAATATT
  TACTTTGGGGATAATCAATATACGCTTGAGGGCGCAGCAAACCATTACTTTGGAACAACC
  GTGAATAAAAATAGTACAACAATGTCTCACATAACAGTTTTACAAAGCGCTATTTTAGCT
  AGTAAAGTCAATGCACCTAGCGTATATAATATCAATAATATGTCAGAGAATTTTACGCAA
  CGTGTAAGCACGAACTTAGAAAAAATGAAGCAACAAAATTATATCAATGAAACACAATAT
  CAACAGGCTATGTCACAATTAAATCGTTAA
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⊟Protein

⊟General

• locus tag: NWMN_1766 [new locus tag: NWMN_RS10100 ]
• symbol: NWMN_1766
• description: glycosyltransferase
• length: 269
• theoretical pI: 9.82726
• theoretical MW: 31459.4
• GRAVY: -0.664312

⊟Function

• reaction:
  EC 2.4.1.129?  ExPASy
  Peptidoglycan glycosyltransferase (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 199.4)
  and 5 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1B (TIGR02071; HMM-score: 131.8)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan monofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 127.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 114.3)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides oligosaccharide repeat unit polymerase (TIGR04370; HMM-score: 14.2)
  YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 13.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 186.5)
  and 2 more
  no clan defined Orf78; Orf78 (ac78) (PF06024; HMM-score: 18.6)
  Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 9.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 7
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001771
  • TAT(Tat/SPI): 0.000242
  • LIPO(Sec/SPII): 0.003023
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151221978 NCBI
• RefSeq: YP_001332800 NCBI
• UniProt: A6QI56 UniProt

⊟Protein sequence

• MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILLKILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDERFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVAHRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAILASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications