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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1732 [new locus tag: SACOL_RS08855 ]
- pan locus tag?: SAUPAN004307000
- symbol: SACOL1732
- pan gene symbol?: dnaB
- synonym:
- product: replication initiation and membrane attachment protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1732 [new locus tag: SACOL_RS08855 ]
- symbol: SACOL1732
- product: replication initiation and membrane attachment protein
- replicon: chromosome
- strand: -
- coordinates: 1762986..1764386
- length: 1401
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238557 NCBI
- RefSeq: YP_186569 NCBI
- BioCyc: see SACOL_RS08855
- MicrobesOnline: 913178 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1381ATGGGACGACAAGCCTTCGAATTCGGCTTAAGACCAAAGGATCAATTCAAAGTTATGCAA
CACTTTGATTTAAATACGAATCATTTAGAGGTATTGAATCGACTGTATACACCGCTAATT
GGAACGCAAGCAGTGGGTTTATATCATTTTATGACTCAATTCGTTAAAGAAAGTCATAAT
GAAACATTGATTCTTTCTCATTATATTTTTATGAATGAACTTAAAATTAATTTACTAGAG
TTTAGACAACAAATGGACTTACTTGAAGCGATTGGATTGTTAAAGGCGTTTGTCAAACAT
GATGAACAAGAAACACAATTTGTTTACCAATTAATTCAACCGCCTTCAGCACATTTATTT
TTTAATGATCCAATGTTGTCAATCTTTTTATATAGTGAAGTCGAGCATCGTCGATTTCAT
GAGTTGAAGAAATACTTTGAGTATCAACAAATTGATTTGAGTGAATTTAAACAGGTAACA
AGACAATTTACTGATGTTTTTAAAGTGCCGTCTACAAAAATAGATATCGATACAAGTGAT
ATTCCGATTAATGAACCGTACCAAGGTATCGATTTATCAAATGAATCTTTTGACTTTGAA
ATGTTGAGACAAATGTTAGGTAAACATTTTATTAGTCAAGACATTGTTACCAAAGATGCA
AAGCGTTTAATTACACAACTCGCGACTTTATATGGTTTAACAGCAGATGGCATGAAGCAT
GTTATTTTAAATTCAATTACAAGTGGACAACAGTTATCCTTTGAAGAAATGCGTAAACAA
GCACGCTCGTATTACTTAATGGAACACGAAAATCAAATGCCTAAATTACAGGTGAAATCT
CCTGCTACATCATCTTCGGCGGGCAAATCTTCAGAAGTGAATCCAAAGCCACAAAGTGAT
GAATGGTTTGAGTTACTTGAACAAACGAGTCCAATTGATATGTTAGCTTCTTGGTCTGAA
TCAGAACCAACTATATCGCAAAAGACAATGGTTGAAGAATTAATTGAACGTGAAAAAATG
TCTTTTGGCGTCATTAATATTTTGTTGCAATTTGTCATGTTAAAAGAAGATATGAAACTT
CCTAAAGCGTATATTTTGGAAATAGCATCAAATTGGAAGAAAAAAGGTATTAAGACAGCT
AAAGAAGCATATAATTACGCTAAAAAAGTTAATCAACCTAAAAATGAGGGTTCTAGCGGA
AATTACCAAAAGCGTGGTTCATACTATGGGCAGCGTAATCGTATTTCAAAAGAAAAAACA
CCTAAATGGTTAGAAAATAGAGATAAACCTAGTGAAGAAGATTCGGCTAAAGATAATAGC
GTAGATGATCAACAATTAGAGCAAGATCGACAAGCATTTCTAGATAAATTATCTAAAAAA
TGGGAGGAGGACAGTCAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1732 [new locus tag: SACOL_RS08855 ]
- symbol: SACOL1732
- description: replication initiation and membrane attachment protein
- length: 466
- theoretical pI: 6.12276
- theoretical MW: 54532.6
- GRAVY: -0.661159
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 20.3)Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 20.3)
- TheSEED :
- Helicase loader DnaB
- ⊞PFAM: no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 39.4)and 4 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTRQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: dnaI < SACOL1732 < nrdR
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)