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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0483 [new locus tag: SA_RS02840 ]
  • pan locus tag?: SAUPAN002290000
  • symbol: clpC
  • pan gene symbol?: clpC
  • synonym:
  • product: endopeptidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0483 [new locus tag: SA_RS02840 ]
  • symbol: clpC
  • product: endopeptidase
  • replicon: chromosome
  • strand: +
  • coordinates: 562686..565142
  • length: 2457
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTTATTTGGTAGATTAACTGAACGTGCACAGCGCGTATTAGCACATGCACAAGAAGAA
    GCAATTCGTTTAAATCATTCAAATATAGGAACAGAACACCTATTATTGGGGTTAATGAAA
    GAACCTGAAGGAATTGCTGCAAAAGTATTAGAAAGTTTTAATATCACTGAAGATAAAGTA
    ATTGAAGAAGTTGAAAAATTAATCGGACATGGTCAAGATCATGTTGGTACATTGCATTAT
    ACACCTAGAGCTAAAAAAGTCATTGAATTATCGATGGATGAAGCTAGAAAATTACATCAC
    AATTTTGTTGGAACGGAACATATTTTATTAGGCTTGATTCGTGAAAATGAAGGTGTTGCA
    GCAAGAGTTTTTGCAAATCTAGATTTAAATATTACTAAAGCACGTGCACAAGTTGTGAAA
    GCTTTAGGAAACCCTGAAATGAGTAATAAAAATGCACAAGCTAGTAAGTCAAATAATACT
    CCAACTTTAGATAGTTTAGCTCGTGACTTAACAGTCATTGCCAAAGACGGTACATTAGAT
    CCTGTTATAGGACGTGATAAAGAAATTACACGTGTAATTGAAGTATTAAGTAGACGTACG
    AAAAACAATCCTGTACTTATTGGAGAGCCAGGTGTTGGTAAAACTGCTATTGCTGAAGGT
    TTAGCGCAAGCCATAGTGAATAATGAGGTACCAGAGACATTAAAAGATAAGCGTGTTATG
    TCTTTAGATATGGGAACAGTAGTTGCAGGTACTAAATATCGTGGTGAATTTGAAGAGCGT
    CTGAAAAAGGTTATGGAAGAAATCCAACAAGCAGGTAATGTCATCCTATTTATTGATGAG
    TTGCATACTTTAGTTGGTGCTGGTGGTGCTGAAGGTGCTATCGATGCTTCGAATATTTTG
    AAACCGGCATTAGCACGTGGTGAATTACAATGTATTGGTGCTACTACATTAGATGAATAT
    CGCAAAAATATTGAAAAAGACGCGGCTTTAGAACGTCGTTTCCAACCTGTACAAGTTGAT
    GAACCTTCAGTAGTAGATACAGTTGCTATTTTAAAAGGATTAAGAGATCGTTACGAAGCA
    CACCATCGTATTAATATTTCAGACGAAGCTATTGAAGCAGCTGTTAAATTAAGTAACAGA
    TACGTTTCAGATCGTTTCTTACCAGATAAAGCAATTGATTTAATTGATGAAGCAAGTTCT
    AAAGTAAGACTTAAGAGTCATACGACACCTAATAATTTAAAAGAAATTGAACAAGAAATT
    GAAAAAGTTAAAAATGAAAAAGATGCCGCAGTACATGCTCAAGAGTTTGAAAATGCTGCT
    AACCTGCGTGATAAACAAACAAAACTTGAAAAGCAATATGAAGAAGCTAAAAATGAATGG
    AAGAATGCACAAAATGGCATGTCAACTTCATTGTCAGAAGAAGATATTGCTGAAGTTATT
    GCAGGATGGACAGGTATCCCATTAACTAAAATCAATGAAACAGAATCTGAAAAACTTCTT
    AGTCTAGAAGATACATTACATGAGAGAGTTATTGGGCAAAAAGATGCTGTTAATTCAATC
    AGTAAAGCGGTTAGACGTGCCCGTGCAGGGTTAAAAGATCCTAAACGACCAATTGGTAGC
    TTTATCTTCCTTGGACCAACTGGTGTTGGTAAAACTGAATTAGCTAGAGCTTTAGCTGAA
    TCAATGTTTGGCGATGATGATGCGATGATCCGTGTAGACATGAGTGAATTTATGGAAAAA
    CACGCAGTGAGCCGATTAGTTGGTGCTCCTCCAGGATATGTTGGTCATGATGATGGTGGA
    CAATTAACTGAAAAAGTTAGACGTAAACCATATTCTGTAATTTTATTTGATGAAATTGAA
    AAAGCTCATCCAGATGTATTTAATATTCTATTACAAGTTTTAGATGATGGACATTTGACA
    GATACAAAAGGACGTACAGTTGATTTCAGAAATACAATTATCATAATGACATCAAACGTT
    GGGGCACAAGAATTACAAGATCAACGATTTGCTGGATTCGGTGGTTCAAGTGATGGACAA
    GATTATGAAACAATTCGAAAAACGATGTTAAAAGAATTAAAAAATTCATTCCGTCCAGAA
    TTTTTAAACCGTGTAGATGATATCATTGTATTCCATAAACTAACAAAAGAAGAATTAAAA
    GAAATTGTAACAATGATGGTTAATAAATTAACAAATCGATTATCTGAACAAAACATAAAT
    ATTATTGTTACTGATAAAGCGAAAGACAAAATCGCAGAAGAAGGATATGATCCAGAATAT
    GGTGCAAGACCATTAATTAGAGCGATACAAAAAACTATCGAAGATAATTTAAGTGAATTA
    ATATTAGATGGTAATCAAATTGAAGGTAAGAAAGTTACAGTAGATCATGATGGTAAAGAG
    TTTAAATATGACATTGCTGAACAAACTTCAGAAACTAAAACACCATCGCAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2457

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0483 [new locus tag: SA_RS02840 ]
  • symbol: ClpC
  • description: endopeptidase
  • length: 818
  • theoretical pI: 5.4692
  • theoretical MW: 91036.7
  • GRAVY: -0.464548

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization ATP-dependent chaperone protein ClpB (TIGR03346; HMM-score: 1067.9)
    and 25 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp protease ATP-binding subunit ClpA (TIGR02639; HMM-score: 820.3)
    Cellular processes Cellular processes Pathogenesis type VI secretion ATPase, ClpV1 family (TIGR03345; HMM-score: 789.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VI secretion ATPase, ClpV1 family (TIGR03345; HMM-score: 789.9)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp protease, ATP-binding subunit ClpX (TIGR00382; HMM-score: 45)
    Genetic information processing Protein fate Protein folding and stabilization ATP-dependent Clp protease, ATP-binding subunit ClpX (TIGR00382; HMM-score: 45)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion AAA-ATPase EccA (TIGR03922; HMM-score: 40.5)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides putative ATP-dependent protease (TIGR00764; HMM-score: 35.3)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein K (TIGR02881; HMM-score: 34.6)
    Genetic information processing Protein fate Protein folding and stabilization ATP-dependent protease HslVU, ATPase subunit (TIGR00390; HMM-score: 30.9)
    AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 30.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 29.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 28.3)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 27.6)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 27.6)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides endopeptidase La (TIGR00763; EC 3.4.21.53; HMM-score: 25.2)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 24.8)
    Cellular processes Cellular processes Other gas vesicle protein GvpN (TIGR02640; HMM-score: 24.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 21.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 20.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 20.1)
    CbbX protein (TIGR02880; HMM-score: 16.9)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 11.3)
    Plasmodium falciparum uncharacterized domain TIGR01639 (TIGR01639; HMM-score: 11.1)
    Genetic information processing Transcription Transcription factors RNA polymerase-binding protein DksA (TIGR02420; HMM-score: 7.6)
    Signal transduction Regulatory functions Small molecule interactions RNA polymerase-binding protein DksA (TIGR02420; HMM-score: 7.6)
  • TheSEED  :
    • ATP-dependent Clp protease, ATP-binding subunit ClpC
    Protein Metabolism Protein degradation Proteolysis in bacteria, ATP-dependent  ATP-dependent Clp protease, ATP-binding subunit ClpC
  • ⊞PFAM:
    P-loop_NTPase (CL0023) AAA_2; AAA domain (Cdc48 subfamily) (PF07724; HMM-score: 250.5)
    and 55 more
    AAA_lid (CL0671) AAA_lid_9; AAA lid domain (PF17871; HMM-score: 126.3)
    no clan defined Clp_N; Clp amino terminal domain, pathogenicity island component (PF02861; HMM-score: 119.6)
    AAA_lid (CL0671) ClpB_D2-small; C-terminal, D2-small domain, of ClpB protein (PF10431; HMM-score: 100.3)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 94.8)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 71.7)
    AAA_22; AAA domain (PF13401; HMM-score: 47.6)
    Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 46.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 44.3)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 35.4)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 30.1)
    TniB; Bacterial TniB protein (PF05621; HMM-score: 27.6)
    AAA_14; AAA domain (PF13173; HMM-score: 27)
    AAA_18; AAA domain (PF13238; HMM-score: 26.8)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 26.4)
    RuvB_N; Holliday junction DNA helicase RuvB P-loop domain (PF05496; HMM-score: 26.3)
    Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 25.2)
    AAA_lid (CL0671) UVR; UvrB/uvrC motif (PF02151; HMM-score: 24.9)
    P-loop_NTPase (CL0023) RNA_helicase; RNA helicase (PF00910; HMM-score: 23.5)
    AAA_28; AAA domain (PF13521; HMM-score: 23.3)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 21.9)
    NACHT; NACHT domain (PF05729; HMM-score: 21.8)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 21.6)
    VapE-like_dom; Virulence-associated protein E-like domain (PF05272; HMM-score: 21.5)
    AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 21.4)
    AAA_3; ATPase family associated with various cellular activities (AAA) (PF07726; HMM-score: 21.1)
    AAA_24; AAA domain (PF13479; HMM-score: 21.1)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 19.8)
    Torsin; Torsin (PF06309; HMM-score: 19.8)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 19.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 19.2)
    AAA_33; AAA domain (PF13671; HMM-score: 18.8)
    AAA_25; AAA domain (PF13481; HMM-score: 18.3)
    ABC_tran; ABC transporter (PF00005; HMM-score: 18)
    NBD_SMAX1; SMAX1 nucleotide binding domain (PF23569; HMM-score: 17.7)
    KTI12; Chromatin associated protein KTI12 (PF08433; HMM-score: 17.6)
    bpMoxR; MoxR domain in the MoxR-vWA-beta-propeller ternary systems (PF20030; HMM-score: 17.2)
    Ras; Ras family (PF00071; HMM-score: 17.1)
    Sigma54_activ_2; Sigma-54 interaction domain (PF14532; HMM-score: 16.8)
    AAA_30; AAA domain (PF13604; HMM-score: 15.8)
    DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 15.6)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 15.4)
    AAA_19; AAA domain (PF13245; HMM-score: 15)
    no clan defined DUF1002; Protein of unknown function (DUF1002) (PF06207; HMM-score: 14.9)
    P-loop_NTPase (CL0023) Parvo_NS1; Parvovirus non-structural protein NS1 (PF01057; HMM-score: 14.3)
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 13.5)
    PhoH; PhoH-like protein (PF02562; HMM-score: 13.4)
    PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 13.1)
    AAA_23; AAA domain (PF13476; HMM-score: 12)
    no clan defined DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 11.3)
    P-loop_NTPase (CL0023) AAA_17; AAA domain (PF13207; HMM-score: 10.9)
    UIM (CL0702) UIM; Ubiquitin interaction motif (PF02809; HMM-score: 9.7)
    no clan defined CHD5; CHD5-like protein (PF04420; HMM-score: 9.5)
    P-loop_NTPase (CL0023) TIP49; TIP49 P-loop domain (PF06068; HMM-score: 9.5)
    DUF87; Helicase HerA, central domain (PF01935; HMM-score: 8.8)
    no clan defined ABC_tran_CTD; ABC transporter C-terminal domain (PF16326; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.95
    • Cytoplasmic Membrane Score: 0.05
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9638
    • Cytoplasmic Membrane Score: 0.0059
    • Cell wall & surface Score: 0.0021
    • Extracellular Score: 0.0282
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002933
    • TAT(Tat/SPI): 0.000396
    • LIPO(Sec/SPII): 0.000411
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEGIAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNFVGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNTPTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIAEGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVILFIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRFQPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAIDLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLEKQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHERVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDDDAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHPDVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDYETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNINIIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDGKEFKYDIAEQTSETKTPSQA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell: data available for COL
  • ⊟interaction partners:
    SA1984(asp23)alkaline shock protein 23  [2] (data from MRSA252)
    SA0723(clpP)ATP-dependent Clp protease proteolytic subunit  [2] (data from MRSA252)
    SA2074(modA)molybdate-binding protein  [2] (data from MRSA252)
    SA1244(odhB)dihydrolipoamide succinyltransferase  [2] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [2] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [2] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [2] (data from MRSA252)
    SA0218(pflB)formate acetyltransferase  [2] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [2] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [2] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [2] (data from MRSA252)
    SA2016(rpsI)30S ribosomal protein S9  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA1797hypothetical protein  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CtsR (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ Jump up to: 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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