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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0839 [new locus tag: SACOL_RS04315 ]
- pan locus tag?: SAUPAN002707000
- symbol: pgk
- pan gene symbol?: pgk
- synonym:
- product: phosphoglycerate kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0839 [new locus tag: SACOL_RS04315 ]
- symbol: pgk
- product: phosphoglycerate kinase
- replicon: chromosome
- strand: +
- coordinates: 865991..867181
- length: 1191
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238653 NCBI
- RefSeq: YP_185713 NCBI
- BioCyc: see SACOL_RS04315
- MicrobesOnline: 912312 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGGCTAAAAAAATTGTTTCTGATTTAGATCTTAAAGGTAAAACAGTCCTAGTACGTGCT
GATTTTAACGTACCTTTAAAAGACGGTGAAATTACTAATGACAACCGTATCGTTCAAGCT
TTACCTACAATTCAATACATCATCGAACAAGGTGGTAAAATCGTACTATTTTCACATTTA
GGTAAAGTGAAAGAAGAAAGTGATAAAGCAAAATTAACTTTACGTCCAGTTGCTGAAGAC
TTATCTAAGAAATTAGATAAAGAAGTTGTTTTCGTACCAGAAACACGCGGCGAAAAACTT
GAAGCTGCTATTAAAGACCTTAAAGAAGGCGACGTATTATTAGTTGAAAATACACGTTAT
GAAGATTTAGACGGTAAAAAAGAATCTAAAAATGATCCAGAATTAGGTAAATACTGGGCA
TCTTTAGGTGATGTGTTTGTAAATGATGCTTTTGGTACTGCGCATCGTGAGCATGCATCT
AATGTTGGTATTTCTACACATTTAGAAACTGCAGCTGGATTCTTAATGGATAAAGAAATT
AAGTTTATTGGCGGCGTAGTTAACGATCCACATAAACCAGTTGTTGCTATTTTAGGTGGA
GCAAAAGTATCTGACAAAATTAATGTCATCAAAAACTTAGTTAACATAGCTGATAAAATT
ATCATCGGCGGAGGTATGGCTTATACTTTCTTAAAAGCGCAAGGTAAAGAAATTGGTATT
TCATTATTAGAAGAAGATAAAATCGACTTCGCAAAAGATTTATTAGAAAAACATGGTGAT
AAAATTGTATTACCAGTAGACACTAAAGTTGCTAAAGAATTTTCTAATGATGCCAAAATC
ACTGTAGTACCATCTGATTCAATTCCAGCAGACCAAGAAGGTATGGATATTGGACCAAAC
ACTGTAAAATTATTTGCAGATGAATTAGAAGGTGCGCACACTGTTGTATGGAATGGACCT
ATGGGTGTATTCGAGTTCAGTAACTTTGCACAAGGTACAATTGGTGTATGTAAAGCAATT
GCAAACCTTAAAGATGCAATTACGATTATCGGTGGCGGTGATTCAGCTGCAGCAGCAATC
TCTTTAGGTTTTGAAAATGACTTCACTCATATTTCAACTGGTGGCGGCGCGTCATTAGAG
TACCTAGAAGGTAAAGAATTGCCTGGTATCAAAGCAATCAATAATAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0839 [new locus tag: SACOL_RS04315 ]
- symbol: Pgk
- description: phosphoglycerate kinase
- length: 396
- theoretical pI: 4.96143
- theoretical MW: 42601.4
- GRAVY: -0.126263
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHASNVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3] [4]
- quantitative data / protein copy number per cell: 6687 [5]
- ⊟interaction partners:
SACOL1960 (gatB) aspartyl/glutamyl-tRNA amidotransferase subunit B [6] (data from MRSA252) SACOL1102 (pdhA) pyruvate dehydrogenase complex E1 component subunit alpha [6] (data from MRSA252) SACOL1103 (pdhB) pyruvate dehydrogenase complex E1 component subunit beta [6] (data from MRSA252) SACOL1104 (pdhC) branched-chain alpha-keto acid dehydrogenase E2 [6] (data from MRSA252) SACOL1105 (pdhD) dihydrolipoamide dehydrogenase [6] (data from MRSA252) SACOL2206 (rpsI) 30S ribosomal protein S9 [6] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: 27.34 h [7]
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑ Jump up to: 6.0 6.1 6.2 6.3 6.4 6.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)