PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

Views

More

• Read
• Edit
• History
• Edit source

⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_02239
pan locus tag^?: SAUPAN005188000
symbol: SAOUHSC_02239
pan gene symbol^?: int3
• synonym:
• product: integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_02239
• symbol: SAOUHSC_02239
• product: integrase
• replicon: chromosome
• strand: +
• coordinates: 2073537..2074574
• length: 1038
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 3919661 NCBI
• RefSeq: YP_500723 NCBI
• BioCyc: G1I0R-2115 BioCyc
• MicrobesOnline: 1290679 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAA
  AGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAG
  TTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGCAGAATACTTC
  GAAAATTGGATTAAAACATATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTAT
  AGAAATGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATA
  AATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACT
  ATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTT
  ATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAA
  CAAGAAAAATTCATCTCGGTAACTGAATATGAAATACTAAAAGATCACGTCAGAAAGAAG
  AGAACTCGTTCATCATTAGCGCTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGT
  GCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAG
  CGAAAAACCGATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATT
  ATGGACGTCATAAGTACATTTGCAATTAGCTATGATGGTTACATTTTCAAAGAAGCCGGA
  TCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAAT
  ATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAA
  GGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATAAAAATATAGCAATAACTACA
  TCCGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAA
  ATTTTAGAAAGTATGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1038
  

⊟Protein

⊟General

• locus tag: SAOUHSC_02239
• symbol: SAOUHSC_02239
• description: integrase
• length: 345
• theoretical pI: 10.2787
• theoretical MW: 40236.1
• GRAVY: -0.665797

⊟Function

• ⊞TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 73.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 71.3)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 44.4)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 44.4)
• TheSEED  :
  • Phage integrase
• ⊞PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 79.3)
  and 7 more
  l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 40.8)
  Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 36.4)
  Phage_int_M; Phage integrase central domain (PF22022; HMM-score: 26.8)
  Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 18.3)
  MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 16.2)
  no clan defined DUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 14.2)
  GAP (CL0409) RhoGAP; RhoGAP domain (PF00620; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8807
  • Cytoplasmic Membrane Score: 0.004
  • Cell wall & surface Score: 0.0033
  • Extracellular Score: 0.1119
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024843
  • TAT(Tat/SPI): 0.000581
  • LIPO(Sec/SPII): 0.004792
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 88195912 NCBI
• RefSeq: YP_500723 NCBI
• UniProt: Q2FWP2 UniProt
• STRING: 93061.SAOUHSC_02239 STRING

⊟Protein sequence

• MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNELRSGFNIDNYITLAEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLQAINNALKSACRVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• predicted SigA promoter  : SAOUHSC_02235 > S862 > SAOUHSC_02236 > SAOUHSC_02237 > SAOUHSC_02239 > S863 > SAOUHSC_02240

⊟Regulation

• ⊞regulator: SigH* regulon

  SigH*

  (sigma factor)

  controlling competence and phage integrase genes;

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications