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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
  • pan locus tag?: SAUPAN005188000
  • symbol: int
  • pan gene symbol?: int3
  • synonym:
  • product: integrase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
  • symbol: int
  • product: integrase
  • replicon: chromosome
  • strand: +
  • coordinates: 2126612..2127649
  • length: 1038
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGTATGAAGGAAAA
    AGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAG
    TTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTC
    GAAAATTGGATTAAAACGTATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTAT
    AGAAATGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATA
    AATAGACAAGTTTATCAGAAATTCATAAACGATTATTCAAAAGAACACGCAAAAGAAACT
    ATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGGCTT
    ATTTTTAAAAATCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAA
    CAAGAAAAATTCATCTCGGTAACTGAATATGAAATACTAAAAGATCACGTCAGAAAGAAG
    AGAACTCGTTCATCATTAGCGCTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGT
    GCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAG
    CGAAAAACCGATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATT
    ATGGACGTCATAAGTACATTTGCAATTAGCTATGATGGTTACATTTTCAAAGAAGCCGGA
    TCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAAT
    ATATCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAA
    GGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATAAAAATATAGCAATAACTACA
    TCCGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAA
    ATTTTAGAAAGTATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
  • symbol: Int
  • description: integrase
  • length: 345
  • theoretical pI: 10.2239
  • theoretical MW: 40319.1
  • GRAVY: -0.672464

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 70.5)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 42.6)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 42.6)
  • TheSEED  :
    • Phage integrase
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 79.4)
    and 8 more
    l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 40.4)
    Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 36.3)
    MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 19.2)
    l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 16.1)
    no clan defined DUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 14.4)
    MACRO (CL0223) Macro; Macro domain (PF01661; HMM-score: 13.3)
    no clan defined NblA; Phycobilisome degradation protein nblA (PF04485; HMM-score: 13.1)
    AEP (CL0243) Replicase; Replicase family (PF03090; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018998
    • TAT(Tat/SPI): 0.000588
    • LIPO(Sec/SPII): 0.00368
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNELRSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSACRVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

This is the integrase for prophage ΦFPR3. It is a site-specific class Sa3int integrase that recognizes the TGTATCCAAACTGG attB sequence and catalyzes either integration or excision of a prophage, typically between two copies of the attB sequence. The classical attB sequence for Sa3int prophage is located within beta hemolysin (hlb), sometimes annotated as sphingomyelinase (sph). However, the integrase can accommodate an impressive number of mismatches and can integrate in other locations such as within ebh (GGTTACCATTTAGG), cidA (TACCGCTAACTTGG) or elsewhere, albeit at a much lower efficiency.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ D Coleman, J Knights, R Russell, D Shanley, T H Birkbeck, G Dougan, I Charles
    Insertional inactivation of the Staphylococcus aureus beta-toxin by bacteriophage phi 13 occurs by site- and orientation-specific integration of the phi 13 genome.
    Mol Microbiol: 1991, 5(4);933-9
    [PubMed:1830359] [WorldCat.org] [DOI] (P p)    Christiane Goerke, Roman Pantucek, Silva Holtfreter, Berit Schulte, Manuel Zink, Dorothee Grumann, Barbara M Bröker, Jiri Doskar, Christiane Wolz
    Diversity of prophages in dominant Staphylococcus aureus clonal lineages.
    J Bacteriol: 2009, 191(11);3462-8
    [PubMed:19329640] [WorldCat.org] [DOI] (I p)    Helena Leinweber, Raphael N Sieber, Jesper Larsen, Marc Stegger, Hanne Ingmer
    Staphylococcal Phages Adapt to New Hosts by Extensive Attachment Site Variability.
    mBio: 2021, 12(6);e0225921
    [PubMed:34872344] [WorldCat.org] [DOI] (I p)
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