PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
• pan locus tag^?: SAUPAN005188000
• symbol: int
• pan gene symbol^?: int3
• synonym:
• product: integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
• symbol: int
• product: integrase
• replicon: chromosome
• strand: +
• coordinates: 2126612..2127649
• length: 1038
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913459 NCBI
• RefSeq: YP_494623 NCBI
• BioCyc: see SAUSA300_RS10835
• MicrobesOnline: 1293487 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGTATGAAGGAAAA
  AGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAG
  TTAAATGAGTTAAGAAGTGGTTTTAATATAGATAACTATATAACTCTTGAAGAATACTTC
  GAAAATTGGATTAAAACGTATAAACAACCTGTTGTTAAAGAAAATACCTACCGTCATTAT
  AGAAATGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATA
  AATAGACAAGTTTATCAGAAATTCATAAACGATTATTCAAAAGAACACGCAAAAGAAACT
  ATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGGCTT
  ATTTTTAAAAATCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAA
  CAAGAAAAATTCATCTCGGTAACTGAATATGAAATACTAAAAGATCACGTCAGAAAGAAG
  AGAACTCGTTCATCATTAGCGCTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGT
  GCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAG
  CGAAAAACCGATACTTCCCCTAGATATATCAGTATCGCTAAATCTGATATGAAACACATT
  ATGGACGTCATAAGTACATTTGCAATTAGCTATGATGGTTACATTTTCAAAGAAGCCGGA
  TCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAAT
  ATATCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAA
  GGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATAAAAATATAGCAATAACTACA
  TCCGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAA
  ATTTTAGAAAGTATGTAA
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  1038
  

⊟Protein

⊟General

• locus tag: SAUSA300_1972 [new locus tag: SAUSA300_RS10835 ]
• symbol: Int
• description: integrase
• length: 345
• theoretical pI: 10.2239
• theoretical MW: 40319.1
• GRAVY: -0.672464

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 70.5)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 42.6)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 42.6)
• TheSEED  :
  • Phage integrase
• PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 79.4)
  and 8 more
  l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 40.4)
  Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 36.3)
  MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 19.2)
  l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 16.1)
  no clan defined DUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 14.4)
  MACRO (CL0223) Macro; Macro domain (PF01661; HMM-score: 13.3)
  no clan defined NblA; Phycobilisome degradation protein nblA (PF04485; HMM-score: 13.1)
  AEP (CL0243) Replicase; Replicase family (PF03090; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.018998
  • TAT(Tat/SPI): 0.000588
  • LIPO(Sec/SPII): 0.00368
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162325 NCBI
• RefSeq: YP_494623 NCBI
• UniProt: A0A0H2XKD2 UniProt

⊟Protein sequence

• MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNELRSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSACRVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: int > SAUSA300_1973

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications