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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01520 [old locus tag: SA0258 ]
  • pan locus tag?: SAUPAN001154000
  • symbol: SA_RS01520
  • pan gene symbol?: rbsK
  • synonym:
  • product: ribokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS01520 [old locus tag: SA0258 ]
  • symbol: SA_RS01520
  • product: ribokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 312952..313866
  • length: 915
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (312952..313866) NCBI
  • BioCyc: SA_RS01520 BioCyc
  • MicrobesOnline: see SA0258

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACCAACAAAGTTGTTATTTTAGGTTCAACGAATGTCGATCAATTTTTAACAGTTGAA
    AGATATGCACAACCAGGCGAAACATTACATGTTGAAGAAGCACAAAAAGCATTCGGCGGA
    GGTAAAGGTGCCAACCAGGCTATTGCCACTGCACGCATGCAAGCAGACACAACATTTATT
    ACTAAAATTGGCACTGATGGCGTTGCTGATTTCATCTTAGAAGATTTTAAAGCGGCTCAT
    ATTGATACATCATATATTATCAAAACAACTGAAGCAAAAACGGGCCAAGCCTTTATCACT
    GTGAATGCAGAAGGACAAAACACCATCTATGTTTATGGTGGTGCGAATATGACGATGACA
    CCTGAAGATGTTATTAACGCAAAAGACGCTATAATCAATGCAGACTTTGTCGTTGCACAA
    TTAGAAGTACCCATCCCGGCTATTATATCTGCATTTGAAATTGCCAAGGCACATGGTGTG
    ACGACAGTATTAAATCCTGCACCAGCGAAAGCATTACCTAATGAATTATTATCATTAATC
    GATATTATTGTGCCAAACGAAACAGAAGCCGAATTGTTATCTGGGATTAAAGTAACTAAT
    GAACAATCTATGAAAGACAATGCCAATTACTTTTTATCTTTAGGCATTAAGACTGTTTTG
    ATTACGCTAGGTAAGCAAGGTACATATTTTGCTACTAAAAATCAAAGCCAACACATCGAA
    GCTTATAAAGTAAATGCGATTGATACAACTGCTGCAGGCGACACATTTATTGGTGCATTT
    GTCAGTCGCTTAAACAAGTCGCAAGATAACTTAGCAGATGCTATTGATTTTGGTAATAAA
    GCGAGCTCACTCACTGTACAAAAACACGGCGCGCAAGCATCTATTCCTCTACTAGAAGAA
    GTAAATCAAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS01520 [old locus tag: SA0258 ]
  • symbol: SA_RS01520
  • description: ribokinase
  • length: 304
  • theoretical pI: 4.6011
  • theoretical MW: 32450.5
  • GRAVY: 0.0101974

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.1.15?  ExPASy
    Ribokinase ATP + D-ribose = ADP + D-ribose 5-phosphate
  • ⊞TIGRFAM:
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 382.2)
    and 7 more
    Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 116.5)
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 93.7)
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 86.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 83.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 52.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 26.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 25)
  • TheSEED: see SA0258
  • ⊞PFAM:
    Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 230.5)
    and 3 more
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 52)
    NADP_Rossmann (CL0063) Glyco_hydro_4; Family 4 glycosyl hydrolase (PF02056; HMM-score: 14)
    E-set (CL0159) Adeno_E3_CR1; Adenovirus E3 region protein CR1 (PF02440; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9975
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.002
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014499
    • TAT(Tat/SPI): 0.001126
    • LIPO(Sec/SPII): 0.001169
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446104891 NCBI
  • RefSeq: WP_000182746 NCBI
  • UniProt: see SA0258

⊟Protein sequence[edit | edit source]

  • MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGANQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVNAEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGVTTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKTVLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAIDFGNKASSLTVQKHGAQASIPLLEEVNQV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA, RbsR* see SA0258

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS01520&oldid=87129"
  • This page was last edited on 11 March 2016, at 06:11.
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