⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_0413 [new locus tag: NWMN_RS02330 ]
pan locus tag^?: SAUPAN002146000
symbol: NWMN_0413
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0413 [new locus tag: NWMN_RS02330 ]
• symbol: NWMN_0413
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 461738..463066
• length: 1329
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332098 NCBI
• RefSeq: YP_001331447 NCBI
• BioCyc:
• MicrobesOnline: 3705944 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGTTGAGTAGGAAGTATAAAATAGATTTAAAAGCAATTAATCAAAATACTGAGTCTACA
  AGTGCTATTTCGAAAGCGTCATACGAAGTTGAGAATGCTAATAATAATGGTCTATCAAAA
  AGAGACGTGATTAATCAATTTAATGATTTGAAAAAAATGAAAAAATTCCCTTCTAATCTT
  GAATATGTTGATAGTTACACTGATTCTCTTACTGGAGTAACAACTTCTGCTTTTTTAAAT
  AAAGATACAGGCAAAGTAACTCTCGGGATGACTGGGACTAATTTACAAGACGAAGCCTTT
  AAAAAGTTAAAAGAAGGTGAATTTTCAAGACAAAATGTTACCAATGCTTTGGAAACAGTT
  AAAGATGGATATGCAGATCTTAAAATATTATATTCTCCTGCATCTGATCAAAACTATAGA
  TATGCGAATACACAAGAATTTATAAATAAAATAAAAAGTAAGTATGACATTGATTTTATT
  ACTGGACATTCACTAGGTGGAAGAGATGCGGTAGTTCTAGGAATGAGTAATGGTATTCCG
  AACATTGTGGTTTATAATCCAGCTCCTATTTCTATAACTAGTTTGAATCCTAATTCCCCA
  GATGGAAAACGTTTATTAGAATTATATAAAAATTATAAAGGTAATATTACTAGGTTTGTT
  GCAGAAAATGATGCATTGACAGAAAATCTGAAGAAATATAAGCATTATGTTTTTTTCGGT
  AATGATAAAGTCTTTAAAAATGGTAAAGGTCATGAAATGGAAGGCTTTCTGACCGAAGAA
  GAACAAAAAGCTATAAAAAAAGAACTTAAAAAACTACAAGGTTATGCAGAAGAAAATAAT
  AAGTCATTTGTAAAGAATTCAAATAATGCTATCTCTAAATTAGCTAGTATAGAATTACTT
  AGAGCAAATATGATGACTACAAATGGAGGAGGATTATCTTCTTCGCAGCAAAAAGTTTTA
  GAAAGTTTAACAGCTTTAACAATTGCGCAGTCATTCAGTCAACTGATAGACGATGAAATT
  AATCAAATCAAAAAAATGTATAATGAAAAGAAAAAGAAATTTGGAAAAAATTGGGAAGAC
  GCTCAAAAAGCTGGAAAAGCTGTAGGTGAAGATTTGAGTGTAAATGGAGTTCTCAATGCT
  TTAGATGAAGGTCAAGTGAATGAAAGTAGTATGGTAAGAGAACCTGAACAAATGATATCT
  GCAAAAGAAAGACAACTTTCAACGATAGGGTCTTCTGTATCAAATTATATTATGAGAGTT
  AGACTCAGTATTAATGAAATCGTTGATAAAGATCAAGTGCTTGCATCACAAATAGGTGGA
  TTACTATGA
  60
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  1140
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  1260
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  1329
  

⊟Protein

⊟General

• locus tag: NWMN_0413 [new locus tag: NWMN_RS02330 ]
• symbol: NWMN_0413
• description: hypothetical protein
• length: 442
• theoretical pI: 9.34275
• theoretical MW: 49261.3
• GRAVY: -0.592534

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Degradation esterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 11.3)
• TheSEED  :
  • FIG01107856: hypothetical protein
• ⊞⊞PFAM:
  AB_hydrolase (CL0028) Lipase_3; Lipase (class 3) (PF01764; HMM-score: 15.1)
  RXXXE-like (CL0797) DUF5677; Family of unknown function (DUF5677) (PF18928; HMM-score: 12.3)
  and 2 more
  no clan defined Erf4; Golgin subfamily A member 7/ERF4 family (PF10256; HMM-score: 11.6)
  Apolipoprotein (CL0725) Apo-CIII; Apolipoprotein CIII (Apo-CIII) (PF05778; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0003
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.9992
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01816
  • TAT(Tat/SPI): 0.002573
  • LIPO(Sec/SPII): 0.003406
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220625 NCBI
• RefSeq: YP_001331447 NCBI
• UniProt: A0A0H3KAX8 UniProt

⊟Protein sequence

• MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVINQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKKLKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFITGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRFVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAEENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQLIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVREPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: lpl9nm > NWMN_0413 > NWMN_0414 > NWMN_0415

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications