PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_01012
pan locus tag^?: SAUPAN003281000
symbol: SAOUHSC_01012
pan gene symbol^?: purQ
• synonym:
• product: phosphoribosylformylglycinamidine synthase I

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_01012
• symbol: SAOUHSC_01012
• product: phosphoribosylformylglycinamidine synthase I
• replicon: chromosome
• strand: +
• coordinates: 981888..982559
• length: 672
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 3920273 NCBI
• RefSeq: YP_499562 NCBI
• BioCyc: G1I0R-952 BioCyc
• MicrobesOnline: 1289475 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTTGCGGTTCTTGTTTTTCCAGGTTCGAATTGTGATAGAGACATGTTTAATGCT
  GCTATTAAAAGTGGTGTTGAAGCGGAATATGTAGATTATAGAGAAACATCACTAAGTGGA
  TTTGATGGCGTACTTATTCCTGGTGGATTTTCATTCGGGGATTACTTAAGATCTGGGGCA
  ATGGCTAGTGTAGCGCCGATTATTTCGGAAGTTAAACGTCTTGCAGCTGAAGGTAAGCCA
  GTATTAGGTGTTTGTAATGGGTTTCAAATTTTAACTGAAATAGGCTTATTACCTGGTGCA
  TTATTGCATAACGATTCACATTTATTTATTAGTAGAAATGAAGAGTTAGAAATAGTGAAT
  AATCAAACGGCATTTACAAATCTTTATGAACAAGGTGAAAAAGTTATATATCCTGTAGCT
  CACGGTGAAGGTCATTATTATTGTACTGATGAAATATATCAACAATTAAAAGCTAACAAT
  CAAATTATTCTGAAATATGTGAATAATCCGAACGGTTCATATGATGATATTGCAGGAATT
  GTTAACGAAAAAGGCAATGTATGTGGCATGATGCCACATCCTGAAAGAGCTTTAGAAACG
  TTGTTAGGTACTGATAGTGGTGTGAAATTATTTGAAGCGATGGTAAAAAGTTGGAGGGAA
  CAACATGTCTAA
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⊟Protein

⊟General

• locus tag: SAOUHSC_01012
• symbol: SAOUHSC_01012
• description: phosphoribosylformylglycinamidine synthase I
• length: 223
• theoretical pI: 4.69597
• theoretical MW: 24526.7
• GRAVY: -0.108969

⊟Function

• ⊞reaction:
  EC 3.5.1.2?  ExPASy
  Glutaminase L-glutamine + H₂O = L-glutamate + NH₃
  EC 6.3.5.3?  ExPASy
  Phosphoribosylformylglycinamidine synthase ATP + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O = ADP + phosphate + 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + L-glutamate
• ⊞TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 325.8)
  and 10 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 83.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 76.2)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 25.8)
  Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 25.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 21.8)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 17.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 17.1)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 16.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 13.2)
  aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 10.2)
• TheSEED  :
  • Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
  Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
• ⊞PFAM:
  Glutaminase_I (CL0014) GATase_5; CobB/CobQ-like glutamine amidotransferase domain (PF13507; HMM-score: 149.9)
  and 5 more
  GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 33.5)
  GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 29.7)
  DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 28.5)
  Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 15.6)
  SNO; SNO glutamine amidotransferase family (PF01174; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9748
  • Cytoplasmic Membrane Score: 0.0003
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0248
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.045141
  • TAT(Tat/SPI): 0.000764
  • LIPO(Sec/SPII): 0.006095
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 88194766 NCBI
• RefSeq: YP_499562 NCBI
• UniProt: Q2FZJ1 UniProt
• STRING: 93061.SAOUHSC_01012 STRING

⊟Protein sequence

• MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGVLIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALLHNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANNQIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSWREQHV

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAOUHSC_01008 > SAOUHSC_01009 > SAOUHSC_01010 > SAOUHSC_01011 > SAOUHSC_01012 > SAOUHSC_01013 > SAOUHSC_01014 > SAOUHSC_01015 > SAOUHSC_01016 > purH > SAOUHSC_01018
  predicted SigA promoter  : S413 > SAOUHSC_01008 > SAOUHSC_01009 > SAOUHSC_01010 > SAOUHSC_01011 > SAOUHSC_01012 > SAOUHSC_01013 > SAOUHSC_01014 > SAOUHSC_01015 > SAOUHSC_01016 > purH > SAOUHSC_01018

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications