NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL

locus tag: SACOL0573 [new locus tag: SACOL_RS02975 ]

pan locus tag^?: SAUPAN002293000

symbol: SACOL0573

pan gene symbol^?: —

synonym:
product: PIN/TRAM domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL0573 [new locus tag: SACOL_RS02975 ]
symbol: SACOL0573
product: PIN/TRAM domain-containing protein
replicon: chromosome
strand: +
coordinates: 594384..595457
length: 1074
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 3238530 NCBI
RefSeq: YP_185459 NCBI
BioCyc: see SACOL_RS02975
MicrobesOnline: 912053 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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1021
GTGAATATCGTTAAACTAATGGTTATTATTATTTACTTAATTATTGGGAGCGCATTAGGA
ATAATTATTATTCCTGAAATTGCAAATGATCTTGGATTACAAAACTCCAGCTTTTTAAAA
AATCACTATGTAGATGGCATTATCGGTAGTATTTTTATGTTCTTAATTTTTGGTGTATTT
ATTAGACGAGTTACTAACGCTATAAAAGGTTTAGAACATTTTATTATGCGTAGAAGTGCT
GTTGAAATACTATTCGCAACAATAGGTTTAATAATCGGATTACTTATTTCTGTTATGGTG
TCGTTTATATTAGAATCAATTGGCAACTCTATTTTTAATCATTTCATTCCTGTCATAATT
ACGATATTACTATGTTATTTCGGTTTCCAATTTGGCCTTAAAAAACGAGATGAAATGTTA
ATGTTTTTACCTGAGAATATAGCGCGTTCCATGTCACAACATACTAAAAGTGCTACGCCA
AAAATTATCGACACAAGCGCAATTATTGATGGTCGTATTTTAGAAGTCATTCGTTGCGGT
TTTATCGATGGCAATATTTTAATTCCACAAGGTGTTATTAATGAATTACAAATTGTTGCA
GATTCAAATGACAGTGTTAAACGTGAAAAGGGTAAAAGAGGCTTAGATATTTTAAATGAA
TTGTATGATTTAGACTATCCTACAAAGGTTATACATCCAACTAAAACACATAGTGATATT
GATACGATGTTATTAAAATTAGCAAAACAATATCATGCAAGTATTATAACGACAGATTTC
AACCTAAATAAAGTTTGTCATGTACATGGTATCAAAGCATTAAATGTTAATGATTTATCA
GAAGCAATCAAACCTAATGTACATCAAGGTGATCAACTGCATATTTTACTGACAAAAATG
GGTAAGGAGCCTGGTCAGGCAGTAGGATATCTAGATGATGGTACGATGGTAGTTGTTGAT
AATGCTAAAAATCTTATTGGCAGTCATGTCAATTTAGAAGTAGTCAGCTTATTGCAAACA
TCTTCAGGAAGAATTGTTTTTGCTAAAAAAATCGAAGATACAGTATCATTATAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL0573 [new locus tag: SACOL_RS02975 ]
symbol: SACOL0573
description: PIN/TRAM domain-containing protein
length: 357
theoretical pI: 7.4154
theoretical MW: 39586.4
GRAVY: 0.413165

⊟Function[edit | edit source]

⊞⊞TIGRFAM:
RNA ligase partner, MJ_0950 family (TIGR03875; HMM-score: 15.4)
and 1 more
Metabolism Energy metabolism Other tetrahydromethanopterin S-methyltransferase, subunit C (TIGR01148; EC 2.1.1.86; HMM-score: 11.2)
TheSEED :
- Membrane-associated protein containing RNA-binding TRAM domain and ribonuclease PIN-domain, YacL B.subtilis ortholog
Stress Response Heat shock Heat shock dnaK gene cluster extended Membrane-associated protein containing RNA-binding TRAM domain and ribonuclease PIN-domain, YacL B.subtilis ortholog
⊞⊞PFAM:
PIN (CL0280) PIN; PIN domain (PF01850; HMM-score: 23)
and 8 more
OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 17.6)
PIN (CL0280) PIN_9; PIN like domain (PF18477; HMM-score: 15.3)
PIN_5; PINc domain ribonuclease (PF08745; HMM-score: 14.4)
PIN_4; PIN domain (PF13638; HMM-score: 14.1)
no clan defined MtrC; Tetrahydromethanopterin S-methyltransferase, subunit C (PF04211; HMM-score: 11.6)
LysE (CL0292) Colicin_V; Colicin V production protein (PF02674; HMM-score: 10.1)
BPD_transp_1 (CL0404) FtsX; FtsX-like permease C-terminal (PF02687; HMM-score: 7.6)
no clan defined Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 7.4)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞⊞
PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.07
- Cytoplasmic Membrane Score: 9.55
- Cellwall Score: 0.18
- Extracellular Score: 0.21
- Internal Helices: 4
⊞⊞
DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0.0006
- Cytoplasmic Membrane Score: 0.9965
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0028
⊞⊞
LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
⊞⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.073056
- TAT(Tat/SPI): 0.000271
- LIPO(Sec/SPII): 0.041682
predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

GI: 57651403 NCBI
RefSeq: YP_185459 NCBI
UniProt: A0A0H2WYJ8 UniProt

⊟Protein sequence[edit | edit source]

MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYVDGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSFILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATPKIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDILNELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNVNDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEVVSLLQTSSGRIVFAKKIEDTVSL

⊟Experimental data[edit | edit source]

experimentally validated: PeptideAtlas
protein localization: Integral membrane ^[1] ^[2] ^[3]
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: radA > SACOL0573

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊞Other Information[edit | edit source]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)
↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p)
↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

[pubmed19997597-1] Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)

[pubmed21323324-2] Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p)

[pubmed24439828-3] Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)

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Links