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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1480 [new locus tag: NWMN_RS08335 ]
  • pan locus tag?: SAUPAN004160000
  • symbol: NWMN_1480
  • pan gene symbol?: rsmE
  • synonym:
  • product: 16S ribosomal RNA methyltransferase RsmE

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1480 [new locus tag: NWMN_RS08335 ]
  • symbol: NWMN_1480
  • product: 16S ribosomal RNA methyltransferase RsmE
  • replicon: chromosome
  • strand: -
  • coordinates: 1646441..1647193
  • length: 753
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 5330884 NCBI
  • RefSeq: YP_001332514 NCBI
  • BioCyc:
  • MicrobesOnline: 3707032 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGCAACGTTATTTCATAGACCAAAACGCTGATGTAAGTCAGCGTTTTTTTATTACAAAA
    AAAGAAGATATTCATCATATAACAAATGTAATGAGATATGATGTTGGTAGTAAAATTATT
    TTAACTTTTAATGATCAAACAGTTTATTTATGTGAAATTGCTGACATAACAAGTGATAAA
    ATTGAAGTTGATTTAAAAGAAAAACAAAATATTAATACAGAATTGCCAGTTGATGTTACG
    ATTTGCAGTGGACTAATCAAAGCTGACAAATATGAGTGGTTGCTACAAAAAGCTACTGAA
    TTGGGTGCTTCATCATTTATAGCTGTGAGCATGGAACGTTCAATTGTTAAATTAAACGAG
    GCAAAAGTTGCCAAAAAGATAGAGCGTTGGCAAAAAATAATTAAAGAAGCTGCTGAACAA
    AGCTATCGTTTAGTGATTCCATCTATTCAATTCGAGTCGAATTTAAAATTAATTTGTGAT
    ACTATTGATAATTATGACTATATTCTTATTGCATATGAAGAGGAAGCAAAAGATGGTGAG
    TTAAGCAATTTCAAGCAAACTTTACAACAATTCAATGCTCAGGATAAAGTGTTGATGATT
    TTTGGTCCTGAGGGTGGTTTGTCAGAAAATGAAATTTCATTATTTAGTAATACTAGTACA
    GTTGTTGGTCTTGGGCCGAGAATTTTACGGGCAGAAACTGCGCCATTGTATGCGTTAAGT
    GCAATAAGTTATGAAAAAGAATTAATGGGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1480 [new locus tag: NWMN_RS08335 ]
  • symbol: NWMN_1480
  • description: 16S ribosomal RNA methyltransferase RsmE
  • length: 250
  • theoretical pI: 4.60587
  • theoretical MW: 28469.4
  • GRAVY: -0.1916

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.1.1.193?  ExPASy
    16S rRNA (uracil1498-N3)-methyltransferase S-adenosyl-L-methionine + uracil1498 in 16S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, RsmE family (TIGR00046; EC 2.1.1.-; HMM-score: 204.7)
  • TheSEED  :
    • Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-)
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-)
  • ⊞PFAM:
    SPOUT (CL0098) Methyltrans_RNA; RNA methyltransferase domain (PF04452; HMM-score: 185)
    and 3 more
    PUA (CL0178) PUA_4; RNA methyltransferase PUA domain (PF20260; HMM-score: 52.4)
    SPOUT (CL0098) SpoU_methylase; SpoU rRNA Methylase family (PF00588; HMM-score: 13.6)
    bBprotInhib (CL0354) Staphostatin_A; Staphostatin A (PF09022; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9875
    • Cytoplasmic Membrane Score: 0.003
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0092
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001491
    • TAT(Tat/SPI): 0.000117
    • LIPO(Sec/SPII): 0.000288
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 151221692 NCBI
  • RefSeq: YP_001332514 NCBI
  • UniProt: A0A0H3K8T5 UniProt

⊟Protein sequence[edit | edit source]

  • MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLYALSAISYEKELMG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • NWMN_1479 < NWMN_1480 < prmA < dnaJ < dnaK < grpE < hrcA  [1]

⊟Regulation[edit | edit source]

  • data available for JSNZ

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Arnaud Chastanet, Juliette Fert, Tarek Msadek
    Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol: 2003, 47(4);1061-73
    [PubMed:12581359] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_1480&oldid=67370"
  • This page was last edited on 10 March 2016, at 18:03.
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