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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1773 [new locus tag: NWMN_RS10135 ]
  • pan locus tag?: SAUPAN001709000
  • symbol: NWMN_1773
  • pan gene symbol?: —
  • synonym:
  • product: phage N-acetylglucosamidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1773 [new locus tag: NWMN_RS10135 ]
  • symbol: NWMN_1773
  • product: phage N-acetylglucosamidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1984284..1986182
  • length: 1899
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGATTACCTAATCCAAAAAACAGAAAACCTACAGCTAGTGAAGTAGTAGAGTGGGCT
    TTGTATATCGCTAAAAATAAAATAGCTATTGATGTACCTGGTTCTGGAATGGGAGCACAA
    TGCTGGGATTTACCTAATTATTTACTCGATAAATATTGGGGGTTTAGAACATGGGGAAAT
    GCTGATGCTATGGCTCAAAAATCCAATTATAGAGGTAGAGATTTCAAGATAATTAGAAAT
    ACAAAAGATTTTGTACCACAACCAGGCGACTGGGGTGTTTGGACTGGTGGTTGGGCAGGA
    CATGTAAACATTGTAGTGGGACCATGCACAAAAGACTATTGGTATGGCGTAGATCAAAAC
    TGGTATACAAATAACGCAACAGGAAGTCCACCTTATAAAATTAAACACTCTTATCATGAT
    GGACCAGGTGGAGGGGTTAAATATTTTGTTAGACCACCATATCATCCAGACAAAACTACA
    CCGGCACCTAAACCAGAAGATGATAGTGATGATAACGAAAAAAATAATAAAAAAGTTCCA
    ATTTGGAAAGATGTAACAACTATAAAGTACACTATTTCTAGCCAAGAGGTTAATTATCCA
    GAATATATTTATCACTTTATAGTAGAAGGTAATCGACGACTCGAAAAACCTAAAGGAATA
    ATGATTAGAAACGCACAAACGATGAGCTCGGTAGAAAGTTTATATAACAGTAGGAAGAAA
    TACAAACAGGATGTAGAATATCCCCACTTTTATGTTGATAGACATAATATTTGGGCACCT
    AGAAGAGCTGTATTTGAAGTTCCTAATGAACCTGATTATATAGTTATAGACGTATGTGAA
    GATTATAGTGCGAGTAAAAATGAATTTATTTTTAATGAGATTCACGCAATGGTTGTAGCT
    GTAGATATGATGGCCAAATATGAGATACCTCTAAGTATTGAAAATTTAAAAGTAGACGAC
    AGCATTTGGCGTTCAATGTTGGAACATGTTAATTGGAATATGATTGACAACGGTGTTCCT
    CCTAAAGATAAATACGAAGCATTAGAAAAGGCATTACTTAATATATTTAAAAACAGAGAA
    AAATTATTAAATTCTATAACTAAGCCAACAGTAACAAAATCTAGAATAAAAGTTATGGTA
    GATAATAAAAACGCTGATATAGCGAATGTAAGAGACTCGTCACCAACAGCCAACAATGGT
    TCGGCATCTAAACAACCGCAGATCATAACAGAAACGAGTCCTTATACATTCAAACAAGCA
    CTGGATAAACAAATGGCAAGAGGTAACCCGAAAAAATCTAATGCTTGGGGTTGGGCTAAC
    GCTACACGAGCACAAACGAGTTCAGCAATGAATGTTAAACGAATATGGGAAAGTAACACG
    CAGTGCTACCAAATGCTTAATTTAGGCAAGTATCAAGGTGTTTCAGTTAGCGCACTTAAT
    AAGATACTTAAAGGTAAGGGAACATTGAATAATCAAGGTAAAGCGTTCGCAGAAGCTTGT
    AAAAAGCACAGCATTAATGAAATTTATTTAATCGCGCATGCTTTCTTAGAAAGTGGATAT
    GGAACAAGTAACTTCGCTAACGGAAAAGATGGAGTATACAACTACTTCGGCATTGGTGCT
    TACGACAACAATCCTAACTACGCAATGACGTTTGCAAGGAATAAAGGTTGGACATCTCCA
    GCAAAAGCAATCATGGGCGGTGCTAGCTTCGTAAGAAAGGATTACATCAATAAAGGTCAA
    AACACATTGTACCGAATTAGATGGAATCCTAAGAATCCAGCTACCCACCAATACGCTACT
    GCTATAGAGTGGTGCCAACATCAAGCAAGTACAATCGCTAAGTTATATAAACAAATCGGC
    TTAAAAGGTATCTACTTCACAAGGGATAAATATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1899

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1773 [new locus tag: NWMN_RS10135 ]
  • symbol: NWMN_1773
  • description: phage N-acetylglucosamidase
  • length: 632
  • theoretical pI: 9.77316
  • theoretical MW: 71975.1
  • GRAVY: -0.684335

⊟Function[edit | edit source]

  • TIGRFAM:
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 22.5)
  • ⊞TheSEED  :
    • Phage endo-beta-N-acetylglucosaminidase (EC 3.2.1.96)
    • Phage endolysin N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Murein Hydrolases  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    and 3 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Recycling of Peptidoglycan Amino Acids  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Bifunctional autolysin Atl
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Bifunctional autolysin Atl
  • ⊞PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 50.3)
    and 2 more
    no clan defined DUF5415; Family of unknown function (DUF5415) (PF17436; HMM-score: 13.8)
    Lysozyme (CL0037) SLT_2; Transglycosylase SLT domain (PF13406; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.9998
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.031405
    • TAT(Tat/SPI): 0.001237
    • LIPO(Sec/SPII): 0.002792
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDLPNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHVNIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPDKTTPAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPKGIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVIDVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNMIDNGVPPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRDSSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAMNVKRIWESNTQCYQMLNLGKYQGVSVSALNKILKGKGTLNNQGKAFAEACKKHSINEIYLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMGGASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGIYFTRDKYK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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