PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1772 [new locus tag: NWMN_RS10130 ]
• pan locus tag^?: SAUPAN001708000
• symbol: NWMN_1772
• pan gene symbol^?: —
• synonym:
• product: phage tail fiber

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1772 [new locus tag: NWMN_RS10130 ]
• symbol: NWMN_1772
• product: phage tail fiber
• replicon: chromosome
• strand: -
• coordinates: 1983099..1984271
• length: 1173
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331059 NCBI
• RefSeq: YP_001332806 NCBI
• BioCyc:
• MicrobesOnline: 3707360 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGTACAAAATAAAAGATGTTGAAACGAGAATAAAAAATGATGGTGTTGACTTAGGTGAC
  ATTGGCTGTCGATTTTACACTGAAGATGAAAATACAGCATCTATAAGAATAGGTATCAAT
  GACAAACAAGGTCGTATCGATCTAAAAGCACATGGCTTAACACCTAGATTACATTTGTTT
  ATGGAAGATGGCTCTATATTCAAAAATGAGCCCCTTATTATCGACGATGTTGTAAAAGGG
  TTCCTTACCTACAAGATACCTAAAAAGGTTATCAAACACGCTGGTTATGTTCGTTGTAAG
  CTGTTTTTAGAGAAAGAAGAAGAAAAAATACATGTCGCGAACTTTTCTTTCAATATCGTT
  GATAGTGGCATTGAATCTGCTGTAGCAAAAGAAATCGATGTTAAATTGGTAGATGATGCT
  ATTACGAGAATCTTAAAAGATAACGCGACAGATTTATTGAGCAAAGACTTTAAAGAGAAA
  ATAGATAAAGATGTCATTTCTTACATCGAAAAGAATGAAAGTAGATTTAAAGGTGCGAAA
  GGTGATAAAGGCGAACCGGGACAACCTGGTGCAAAAGGTGAAGCAGGTAAAAAAGGAGAA
  CAAGGCGCACCCGGTAAAAACGGTACTGTAGTATCAATCAATCCTGACACTAAAATGTGG
  CAAATTGATGGTAAAGATACAGATATCAAAGCAGAACCTGAGTTATTGGACAAAATCAAT
  ATCGCAAATGTTGAAGGGTTAGAAGATAAATTGCAAGAAGTTGAAAAAATCAAAGATACA
  ACTCTCAACGACTCTAAAACGTATACGGATACAAAAATTGCTGAACTAGTTGATAGCGCG
  CCTGAATCTATGAACACATTAAGAGAATTAGCAGAAGCAATACAAAACAACTCTATTTCA
  GAAAGTGTATTGCAACAGATTGGCTCAAAAGTTAGTACAGAAGATTTTGAGGAATTCAAA
  CAAACACTAAATGATTTATATGCTCCAAAAAATCATAATCATGACGAGCGGTATGTTTTG
  TCATCTCAAGCTTTTACTAAACAACAAGCGGATAGTTTATATCAACTAAAAAGCGCATCT
  CAACCGACGGTTAAAATTTGGACAGGAACAGAAAATGAATATAACTATATATATCAAAAA
  GACCCTAATACACTTTACTTAATTAAGGGGTGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1173
  

⊟Protein

⊟General

• locus tag: NWMN_1772 [new locus tag: NWMN_RS10130 ]
• symbol: NWMN_1772
• description: phage tail fiber
• length: 390
• theoretical pI: 4.94666
• theoretical MW: 43804
• GRAVY: -0.665128

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage phi 11 orf50 protein homolog
  Phages, Prophages, Transposable elements, Plasmids Phages, Prophages Phage tail fiber proteins  Tail fiber protein [SA bacteriophages 11, Mu50B]
• PFAM:
  E-set (CL0159) BppU_N; BppU N-terminal domain (PF10651; HMM-score: 123.7)
  and 5 more
  no clan defined Collagen; Collagen triple helix repeat (20 copies) (PF01391; HMM-score: 25.6)
  Phage_tail_C; Phage tail tip domain (PF24243; HMM-score: 22.1)
  PIG-S; Phosphatidylinositol-glycan biosynthesis class S protein (PF10510; HMM-score: 14.2)
  PBP_NTD (CL0776) PBP_dimer; Penicillin-binding Protein dimerisation domain (PF03717; HMM-score: 11.5)
  RNase_H (CL0219) RNase_HII; Ribonuclease HII (PF01351; HMM-score: 9.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cell wall & surface
  • Cytoplasmic Score: 0.1457
  • Cytoplasmic Membrane Score: 0.0022
  • Cell wall & surface Score: 0.6812
  • Extracellular Score: 0.1709
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006029
  • TAT(Tat/SPI): 0.000458
  • LIPO(Sec/SPII): 0.001929
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221984 NCBI
• RefSeq: YP_001332806 NCBI
• UniProt: A0A0H3K997 UniProt

⊟Protein sequence

• MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQGRIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKIPKKVIKHAGYVRCKLFLEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEKIDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPDTKMWQIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVEKIKDTTLNDSKTYTDTKIAELVDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFEEFKQTLNDLYAPKNHNHDERYVLSSQAFTKQQADSLYQLKSASQPTVKIWTGTENEYNYIYQKDPNTLYLIKG

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1771 < NWMN_1772 < NWMN_1773

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications