PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1773 [new locus tag: NWMN_RS10135 ]
• pan locus tag^?: SAUPAN001709000
• symbol: NWMN_1773
• pan gene symbol^?: —
• synonym:
• product: phage N-acetylglucosamidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1773 [new locus tag: NWMN_RS10135 ]
• symbol: NWMN_1773
• product: phage N-acetylglucosamidase
• replicon: chromosome
• strand: -
• coordinates: 1984284..1986182
• length: 1899
• essential: unknown

⊟Accession numbers

• Gene ID: 5331060 NCBI
• RefSeq: YP_001332807 NCBI
• BioCyc:
• MicrobesOnline: 3707361 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGGATTACCTAATCCAAAAAACAGAAAACCTACAGCTAGTGAAGTAGTAGAGTGGGCT
  TTGTATATCGCTAAAAATAAAATAGCTATTGATGTACCTGGTTCTGGAATGGGAGCACAA
  TGCTGGGATTTACCTAATTATTTACTCGATAAATATTGGGGGTTTAGAACATGGGGAAAT
  GCTGATGCTATGGCTCAAAAATCCAATTATAGAGGTAGAGATTTCAAGATAATTAGAAAT
  ACAAAAGATTTTGTACCACAACCAGGCGACTGGGGTGTTTGGACTGGTGGTTGGGCAGGA
  CATGTAAACATTGTAGTGGGACCATGCACAAAAGACTATTGGTATGGCGTAGATCAAAAC
  TGGTATACAAATAACGCAACAGGAAGTCCACCTTATAAAATTAAACACTCTTATCATGAT
  GGACCAGGTGGAGGGGTTAAATATTTTGTTAGACCACCATATCATCCAGACAAAACTACA
  CCGGCACCTAAACCAGAAGATGATAGTGATGATAACGAAAAAAATAATAAAAAAGTTCCA
  ATTTGGAAAGATGTAACAACTATAAAGTACACTATTTCTAGCCAAGAGGTTAATTATCCA
  GAATATATTTATCACTTTATAGTAGAAGGTAATCGACGACTCGAAAAACCTAAAGGAATA
  ATGATTAGAAACGCACAAACGATGAGCTCGGTAGAAAGTTTATATAACAGTAGGAAGAAA
  TACAAACAGGATGTAGAATATCCCCACTTTTATGTTGATAGACATAATATTTGGGCACCT
  AGAAGAGCTGTATTTGAAGTTCCTAATGAACCTGATTATATAGTTATAGACGTATGTGAA
  GATTATAGTGCGAGTAAAAATGAATTTATTTTTAATGAGATTCACGCAATGGTTGTAGCT
  GTAGATATGATGGCCAAATATGAGATACCTCTAAGTATTGAAAATTTAAAAGTAGACGAC
  AGCATTTGGCGTTCAATGTTGGAACATGTTAATTGGAATATGATTGACAACGGTGTTCCT
  CCTAAAGATAAATACGAAGCATTAGAAAAGGCATTACTTAATATATTTAAAAACAGAGAA
  AAATTATTAAATTCTATAACTAAGCCAACAGTAACAAAATCTAGAATAAAAGTTATGGTA
  GATAATAAAAACGCTGATATAGCGAATGTAAGAGACTCGTCACCAACAGCCAACAATGGT
  TCGGCATCTAAACAACCGCAGATCATAACAGAAACGAGTCCTTATACATTCAAACAAGCA
  CTGGATAAACAAATGGCAAGAGGTAACCCGAAAAAATCTAATGCTTGGGGTTGGGCTAAC
  GCTACACGAGCACAAACGAGTTCAGCAATGAATGTTAAACGAATATGGGAAAGTAACACG
  CAGTGCTACCAAATGCTTAATTTAGGCAAGTATCAAGGTGTTTCAGTTAGCGCACTTAAT
  AAGATACTTAAAGGTAAGGGAACATTGAATAATCAAGGTAAAGCGTTCGCAGAAGCTTGT
  AAAAAGCACAGCATTAATGAAATTTATTTAATCGCGCATGCTTTCTTAGAAAGTGGATAT
  GGAACAAGTAACTTCGCTAACGGAAAAGATGGAGTATACAACTACTTCGGCATTGGTGCT
  TACGACAACAATCCTAACTACGCAATGACGTTTGCAAGGAATAAAGGTTGGACATCTCCA
  GCAAAAGCAATCATGGGCGGTGCTAGCTTCGTAAGAAAGGATTACATCAATAAAGGTCAA
  AACACATTGTACCGAATTAGATGGAATCCTAAGAATCCAGCTACCCACCAATACGCTACT
  GCTATAGAGTGGTGCCAACATCAAGCAAGTACAATCGCTAAGTTATATAAACAAATCGGC
  TTAAAAGGTATCTACTTCACAAGGGATAAATATAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_1773 [new locus tag: NWMN_RS10135 ]
• symbol: NWMN_1773
• description: phage N-acetylglucosamidase
• length: 632
• theoretical pI: 9.77316
• theoretical MW: 71975.1
• GRAVY: -0.684335

⊟Function

• TIGRFAM:
  flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 22.5)
• TheSEED:
• PFAM:
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 49.2)
  and 3 more
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 29.9)
  no clan defined INCENP_ARK-bind; Inner centromere protein, ARK binding region (PF03941; HMM-score: 12.7)
  Lysozyme (CL0037) SLT_2; Transglycosylase SLT domain (PF13406; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.72
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.031405
  • TAT(Tat/SPI): 0.001237
  • LIPO(Sec/SPII): 0.002792
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221985 NCBI
• RefSeq: YP_001332807 NCBI
• UniProt: A0A0H3KDN2 UniProt

⊟Protein sequence

• MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDLPNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHVNIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPDKTTPAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPKGIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVIDVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNMIDNGVPPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRDSSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAMNVKRIWESNTQCYQMLNLGKYQGVSVSALNKILKGKGTLNNQGKAFAEACKKHSINEIYLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMGGASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGIYFTRDKYK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1771 < NWMN_1772 < NWMN_1773

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications