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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS10025 [old locus tag: NWMN_1753 ]
  • pan locus tag?: SAUPAN004840000
  • symbol: NWMN_RS10025
  • pan gene symbol?: perR
  • synonym:
  • product: transcriptional repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS10025 [old locus tag: NWMN_1753 ]
  • symbol: NWMN_RS10025
  • product: transcriptional repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 1963955..1964401
  • length: 447
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1963955..1964401) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1753

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAGTGTTGAAATAGAATCAATTGAACATGAACTAGAAGAATCAATTGCATCATTGCGA
    CAAGCAGGCGTAAGAATTACACCTCAAAGACAAGCAATATTACGTTATTTAATTTCTTCA
    CATACTCATCCAACAGCTGATGAAATTTATCAAGCACTTTCACCTGATTTTCCAAATATA
    AGTGTTGCGACAATATATAATAACTTAAGAGTGTTTAAAGATATTGGAATTGTAAAAGAA
    TTAACATATGGAGACTCATCAAGTCGATTCGACTTTAATACACATAATCATTATCATATT
    ATATGTGAACAATGTGGTAAGATTGTTGATTTTCAATATCCACAGTTAAATGAAATTGAA
    AGATTAGCTCAGCATATGACTGACTTTGACGTAACACATCATCGAATGGAAATTTATGGA
    GTTTGTAAAGAATGCCAAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    447

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS10025 [old locus tag: NWMN_1753 ]
  • symbol: NWMN_RS10025
  • description: transcriptional repressor
  • length: 148
  • theoretical pI: 5.47242
  • theoretical MW: 17183.2
  • GRAVY: -0.421622

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see NWMN_1753
  • ⊞PFAM:
    HTH (CL0123) FUR; Ferric uptake regulator family (PF01475; HMM-score: 132.9)
    and 11 more
    HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 24.2)
    HTH_11; HTH domain (PF08279; HMM-score: 14.9)
    no clan defined Eplus_motif; E+ motif (PF20430; HMM-score: 14.6)
    HEF_HK; HEF_HK domain (PF19191; HMM-score: 13.7)
    DUF2039; Uncharacterized conserved protein (DUF2039) (PF10217; HMM-score: 13.6)
    E6; Early Protein (E6) (PF00518; HMM-score: 13.2)
    Tbcl_zf (CL0839) zf-dskA_traR; Prokaryotic dksA/traR C4-type zinc finger (PF01258; HMM-score: 13.1)
    no clan defined DASH_Hsk3; DASH complex subunit Hsk3 like (PF08227; HMM-score: 13.1)
    HTH (CL0123) HTH_32; Homeodomain-like domain (PF13565; HMM-score: 12.5)
    Zn_Beta_Ribbon (CL0167) Zn_Ribbon_TF; TFIIB zinc-binding (PF08271; HMM-score: 11.8)
    Ribosomal_L37e; Ribosomal protein L37e (PF01907; HMM-score: 10.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by PerR*, TF important in Oxidative stress response: see NWMN_1753

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8939
    • Cytoplasmic Membrane Score: 0.0034
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.1025
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009002
    • TAT(Tat/SPI): 0.003688
    • LIPO(Sec/SPII): 0.001527
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHPTADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIICEQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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