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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2151 [new locus tag: SACOL_RS11260 ]
  • pan locus tag?: SAUPAN005506000
  • symbol: glmM
  • pan gene symbol?: glmM
  • synonym:
  • product: phosphoglucosamine mutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2151 [new locus tag: SACOL_RS11260 ]
  • symbol: glmM
  • product: phosphoglucosamine mutase
  • replicon: chromosome
  • strand: -
  • coordinates: 2222102..2223457
  • length: 1356
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGAAAATATTTTGGTACAGACGGAGTAAGAGGTGTCGCAAACCAAGAACTAACACCT
    GAATTGGCATTTAAATTAGGAAGATACGGTGGCTATGTTCTAGCACATAATAAAGGTGAA
    AAACACCCACGTGTACTTGTAGGTCGCGATACTAGAGTTTCAGGTGAAATGTTAGAATCA
    GCATTAATAGCTGGTTTGATTTCAATTGGTGCAGAAGTGATGCGATTAGGTATTATTTCA
    ACACCAGGTGTTGCATATTTAACACGCGATATGGGTGCAGAGTTAGGTGTAATGATTTCA
    GCCTCTCATAATCCAGTTGCAGATAATGGTATTAAATTCTTTGGATCAGATGGTTTTAAA
    CTATCAGATGAACAAGAAAATGAAATTGAAGCATTATTGGATCAAGAAAACCCAGAATTA
    CCAAGACCAGTTGGCAATGATATTGTACATTATTCAGATTACTTTGAAGGGGCACAAAAA
    TATTTGAGCTATTTAAAATCAACAGTAGATGTTAACTTTGAAGGTTTGAAAATTGCTTTA
    GATGGTGCAAATGGTTCAACATCATCACTAGCGCCATTCTTATTTGGTGACTTAGAAGCA
    GATACTGAAACAATTGGATGTAGTCCTGATGGATATAATATCAATGAGAAATGTGGCTCT
    ACACATCCTGAAAAATTAGCTGAAAAAGTAGTTGAAACTGAAAGTGATTTTGGGTTAGCA
    TTTGACGGCGATGGAGACAGAATCATAGCAGTAGATGAGAATGGTCAAATCGTTGACGGT
    GACCAAATTATGTTTATTATTGGTCAAGAAATGCATAAAAATCAAGAATTGAATAATGAC
    ATGATTGTTTCTACTGTTATGAGTAATTTAGGTTTTTACAAAGCGCTTGAACAAGAAGGA
    ATTAAATCTAATAAAACTAAAGTTGGCGACAGATATGTAGTAGAAGAAATGCGTCGCGGT
    AATTATAACTTAGGTGGAGAACAATCTGGACATATCGTTATGATGGATTACAATACAACT
    GGTGATGGTTTATTAACTGGTATTCAATTAGCTTCTGTAATAAAAATGACTGGTAAATCA
    CTAAGTGAATTAGCTGGACAAATGAAAAAATATCCACAATCATTAATTAACGTACGCGTA
    ACAGATAAATATCGTGTTGAAGAAAATGTTGACGTTAAAGAAGTTATGACTAAAGTAGAA
    GTAGAAATGAATGGAGAAGGTCGAATTTTAGTAAGACCTTCTGGAACAGAACCATTAGTT
    CGTGTCATGGTTGAAGCAGCAACTGATGAAGATGCTGAAAGATTTGCACAACAAATAGCT
    GATGTGGTTCAAGATAAAATGGGATTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2151 [new locus tag: SACOL_RS11260 ]
  • symbol: GlmM
  • description: phosphoglucosamine mutase
  • length: 451
  • theoretical pI: 4.38454
  • theoretical MW: 49265.2
  • GRAVY: -0.319734

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 5.4.2.10?  ExPASy
    Phosphoglucosamine mutase Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate
  • ⊞TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
    Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
    and 3 more
    Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR03990; EC 5.4.2.10; HMM-score: 346.6)
    Metabolism Energy metabolism Sugars phosphoglucomutase, alpha-D-glucose phosphate-specific (TIGR01132; EC 5.4.2.2; HMM-score: 26.8)
    Metabolism Energy metabolism Other 4-oxalocrotonate tautomerase family enzyme (TIGR00013; EC 5.3.2.-; HMM-score: 13.3)
  • ⊞TheSEED  :
    • FemD, factor involved in methicillin resistance
    • Phosphoglucosamine mutase (EC 5.4.2.10)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Phosphoglucosamine mutase (EC 5.4.2.10)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  Phosphoglucosamine mutase (EC 5.4.2.10)
  • ⊞PFAM:
    PGM_I-III (CL0754) PGM_PMM_I; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I (PF02878; HMM-score: 154.8)
    PGM_PMM_III; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III (PF02880; HMM-score: 128)
    and 3 more
    TBP-like (CL0407) PGM_PMM_IV; Phosphoglucomutase/phosphomannomutase, C-terminal domain (PF00408; HMM-score: 73.8)
    PGM_I-III (CL0754) PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 49.2)
    Periplas_BP (CL0144) Peripla_BP_5; Periplasmic binding protein domain (PF13433; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9892
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0104
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015662
    • TAT(Tat/SPI): 0.00144
    • LIPO(Sec/SPII): 0.00086
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPRVLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISASHNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQKYLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKCGSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQELNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVMMDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDVKEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 2116 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 61.37 h [6]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

L Jolly, F Pompeo, J van Heijenoort, F Fassy, D Mengin-Lecreulx
Autophosphorylation of phosphoglucosamine mutase from Escherichia coli.
J Bacteriol: 2000, 182(5);1280-5
[PubMed:10671448] [WorldCat.org] [DOI] (P p)S Wu, H de Lencastre, A Sali, A Tomasz
A phosphoglucomutase-like gene essential for the optimal expression of methicillin resistance in Staphylococcus aureus: molecular cloning and DNA sequencing.
Microb Drug Resist: 1996, 2(2);277-86
[PubMed:9158773] [WorldCat.org] [DOI] (P p)L Jolly, S Wu, J van Heijenoort, H de Lencastre, D Mengin-Lecreulx, A Tomasz
The femR315 gene from Staphylococcus aureus, the interruption of which results in reduced methicillin resistance, encodes a phosphoglucosamine mutase.
J Bacteriol: 1997, 179(17);5321-5
[PubMed:9286983] [WorldCat.org] [DOI] (P p)

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