From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2153 [new locus tag: SACOL_RS11270 ]
  • pan locus tag?: SAUPAN005508000
  • symbol: SACOL2153
  • pan gene symbol?: dacA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2153 [new locus tag: SACOL_RS11270 ]
  • symbol: SACOL2153
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2224418..2225227
  • length: 810
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGATTTTTCCAACTTTTTTCAAAACCTCAGTACGTTAAAAATTGTAACGAGTATCCTT
    GATTTACTGATAGTTTGGTATGTACTTTATCTTCTCATCACGGTCTTTAAGGGAACTAAA
    GCGATACAATTACTTAAAGGGATATTAGTAATTGTTATTGGTCAGCAGATAAGTATGATA
    TTGAACTTGACTGCAACATCTAAATTATTCGATATCGTTATTCAATGGGGGGTATTAGCT
    TTAATAGTAATATTCCAACCAGAAATTAGACGTGCGTTAGAACAACTTGGTAGAGGTAGC
    TTTTTAAAACGCTATACTTCTAATACGTATAGTAAAGATGAAGAGAAATTGATTCAATCG
    GTTTCAAAGGCTGTGCAATATATGGCTAAAAGACGTATAGGTGCATTAATTGTCTTTGAA
    AAAGAAACAGGTCTTCAAGATTATATTGAAACAGGTATTGCAATGGATTCAAATATTTCG
    CAAGAACTTTTAATTAATGTCTTTATACCTAACACACCTTTACATGATGGTGCAATGATT
    ATTCAAGGCACGAAGATTGCAGCAGCAGCAAGTTATTTGCCATTGTCTGATAGTCCTAAG
    ATATCTAAAAGTTTGGGTACAAGACATAGAGCTGCGGTTGGTATTTCAGAAGTATCTGAT
    GCATTTACCGTTATTGTATCTGAAGAAACTGGTGATATTTCGGTAACATTTGATGGAAAA
    TTACGACGAGACATTTCAAACGAAATTTTTGAAGAGTTGCTTGCTGAACATTGGTTTGGC
    ACACGCTTTCAAAAGAAAGGTGTGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    810

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2153 [new locus tag: SACOL_RS11270 ]
  • symbol: SACOL2153
  • description: hypothetical protein
  • length: 269
  • theoretical pI: 9.19244
  • theoretical MW: 30078.9
  • GRAVY: 0.250929

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.85?  ExPASy
    Diadenylate cyclase 2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00159 family protein (TIGR00159; HMM-score: 295.4)
  • TheSEED  :
    • Cyclic di-AMP synthase CdaA (EC 2.7.7.85)
  • PFAM:
    no clan defined DisA_N; DisA bacterial checkpoint controller nucleotide-binding (PF02457; HMM-score: 150.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00094
    • TAT(Tat/SPI): 0.000102
    • LIPO(Sec/SPII): 0.000511
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQLLKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFLKRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNISQELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEVSDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL2153&oldid=99732"