PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_RS08405 [old locus tag: NWMN_1493 ]
pan locus tag^?: SAUPAN004177000
symbol: NWMN_RS08405
pan gene symbol^?: —
• synonym:
• product: SAM-dependent methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08405 [old locus tag: NWMN_1493 ]
• symbol: NWMN_RS08405
• product: SAM-dependent methyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1661717..1662433
• length: 717
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1661717..1662433) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1493

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCGCAATATGCAGAAATGAGCCTAGTGTACGATCAATTGACTCAAGATCAACCATAT
  GAAAAATGGTTTGAAATTGTAAAAAATCACTGCAAAGATGAATCAAATATTTTAGATATT
  GGATGCGGTACTGGTAGTTTAACAGTTCAATTAGAAGCTTTAGGTAATGTTACTGGTATG
  GATTTAAGTGTTGATATGTTGACTGTTGCAGCTCAAAAATCAGCAAATGTAAATTGGCTT
  GAAGGAGATATGACATCTTTTGATTTGCAACAACAATTTGATATCATCACTATTTTTTGT
  GACTCTCTAAATTATTTGCAAGATGAAACTGCAGTGATTGAAACATTCATCAATGTTTAT
  CATCATCTGACTGATTCAGGTGTATTTATTTTTGATGTACATACTGTTTATAAAATGATG
  ACTTTGTTTAATAATCAAAGTTATATTGATGATAAAGGGGACATTTTTTTAGCATGGGAT
  GCTGTGCAAGGAGATTTACCTTTAAGTGTTTATCATGATATGACATTTTTCATTCGACAT
  GAAGATGAAACATATTCAAGATTTGATGAGTCTCATTTTCAGAGAACATTTGATGAAAAG
  ACATATTTGTCTTGGTTAGCTCAAGTTGGTTTTAAACATGTTGAAACATTTACTGATTTT
  AATATAGATGAACATAATGAAGATGCAGAAAGATTGTTTTTCATTGCGAAAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS08405 [old locus tag: NWMN_1493 ]
• symbol: NWMN_RS08405
• description: SAM-dependent methyltransferase
• length: 238
• theoretical pI: 4.09468
• theoretical MW: 27701.7
• GRAVY: -0.262605

⊟Function

• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 48.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 42.8)
  and 17 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 34.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 33.5)
  Metabolism Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 32.5)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 25.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 24.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 22.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 21.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 20.9)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 17.8)
  Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 17.8)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 17.8)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 17.3)
  methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 15.8)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 15.7)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 14.8)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 14.3)
  2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 11.8)
• TheSEED: see NWMN_1493
• ⊞PFAM:
  NADP_Rossmann (CL0063) Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 60.7)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 49.4)
  and 14 more
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 47.3)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 46)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 40.6)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 37.8)
  Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 29.5)
  MetW; Methionine biosynthesis protein MetW (PF07021; HMM-score: 25.1)
  RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 21.6)
  FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 21.1)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 20.6)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 19.9)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 17.1)
  Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 15.7)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 13.7)
  Methyltransf_8; Hypothetical methyltransferase (PF05148; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9971
  • Cytoplasmic Membrane Score: 0.0003
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0026
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016832
  • TAT(Tat/SPI): 0.0002
  • LIPO(Sec/SPII): 0.000493
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446006974 NCBI
• RefSeq: WP_000084829 NCBI
• UniProt: see NWMN_1493

⊟Protein sequence

• MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGTGSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDSLNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWDAVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFTDFNIDEHNEDAERLFFIAKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications