PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA2464 [new locus tag: SA_RS14110 ]
pan locus tag^?: SAUPAN006427000
symbol: hisI
pan gene symbol^?: hisIE
• synonym:
• product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2464 [new locus tag: SA_RS14110 ]
• symbol: hisI
• product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase
• replicon: chromosome
• strand: -
• coordinates: 2777382..2778014
• length: 633
• essential: no DEG

⊟Accession numbers

• Gene ID: 1125394 NCBI
• RefSeq: NP_375791 NCBI
• BioCyc: see SA_RS14110
• MicrobesOnline: 104817 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACCAAATATAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAAT
  CAAACAAAACAAGTATTGATGTTGGGTTATATGAATCAAGAAGCTTTTGATAAAACGATA
  GAAGATGGTGTGGTATGTTTCTATTCGCGGTCGAAACAACGTCTATGGACAAAAGGTGAA
  ACATCTGGTCATACGCAACTTGTTAAAGATATTCATGTAGATTGCGACAATGATACTATT
  TTAATTGATGTCATACCAAATGGACCAACATGTCATACAGGCAGTCAAAGTTGTTTCAAC
  ACAGAAGTTCCATTTTCAGTGCAAACATTAGCGCAGACAGTTCAAGATAGTGCCCAATCC
  AATAATGAAAAGTCATATACAAAATATTTATTAACAGAAGGTATAGAAAAGATTACAAAA
  AAATACGGTGAAGAAGCTTTTGAAGTCGTAATTGAAGCAATTAAAGGTGACAAAAAAGCA
  TTTGTAAGTGAAGTAGCAGATGAACTTTATCATTTATTTGTCTTGATGCATGCGCTAGGC
  GTCGATTTTTCAGAAATTGAGGCGGAATTAGCGCGTAGACATCATAAGCGCAATAACTTT
  AAAGGTGAACGACAAAATATCGAACAGTGGTAA
  60
  120
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  240
  300
  360
  420
  480
  540
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  633
  

⊟Protein

⊟General

• locus tag: SA2464 [new locus tag: SA_RS14110 ]
• symbol: HisI
• description: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase
• length: 210
• theoretical pI: 5.6078
• theoretical MW: 23943.9
• GRAVY: -0.489048

⊟Function

• ⊞reaction:
  EC 3.5.4.19?  ExPASy
  Phosphoribosyl-AMP cyclohydrolase 1-(5-phospho-beta-D-ribosyl)-AMP + H₂O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide
  EC 3.6.1.31?  ExPASy
  Phosphoribosyl-ATP diphosphatase 1-(5-phospho-beta-D-ribosyl)-ATP + H₂O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family phosphoribosyl-ATP diphosphatase (TIGR03188; EC 3.6.1.31; HMM-score: 90.9)
• ⊞TheSEED  :
  • Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
  • Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
  and 3 more
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
• ⊞PFAM:
  no clan defined PRA-CH; Phosphoribosyl-AMP cyclohydrolase (PF01502; HMM-score: 119.4)
  and 5 more
  MazG (CL0231) PRA-PH; Phosphoribosyl-ATP pyrophosphohydrolase (PF01503; HMM-score: 66.9)
  MazG-like; MazG-like family (PF12643; HMM-score: 20.8)
  Beta_propeller (CL0186) BBS2_Mid; Ciliary BBSome complex subunit 2, middle region (PF14783; HMM-score: 15.6)
  MazG (CL0231) MazG; MazG nucleotide pyrophosphohydrolase domain (PF03819; HMM-score: 14.7)
  TIM_barrel (CL0036) Oxidored_FMN; NADH:flavin oxidoreductase / NADH oxidase family (PF00724; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.977
  • Cytoplasmic Membrane Score: 0.0009
  • Cell wall & surface Score: 0.0029
  • Extracellular Score: 0.0192
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003913
  • TAT(Tat/SPI): 0.000188
  • LIPO(Sec/SPII): 0.000404
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928258 NCBI
• RefSeq: NP_375791 NCBI
• UniProt: P64356 UniProt

⊟Protein sequence

• MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGVVCFYSRSKQRLWTKGETSGHTQLVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTEVPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKAFVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hisI < hisF < SA2466 < hisH < hisB < SA2469 < SA2470 < hisG < hisZ

⊟Regulation

• ⊞regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications