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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS14110 [old locus tag: SA2464 ]
  • pan locus tag?: SAUPAN006427000
  • symbol: SA_RS14110
  • pan gene symbol?: hisI
  • synonym:
  • product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS14110 [old locus tag: SA2464 ]
  • symbol: SA_RS14110
  • product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase
  • replicon: chromosome
  • strand: -
  • coordinates: 2777382..2778014
  • length: 633
  • essential: no DEG

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2777382..2778014) NCBI
  • BioCyc: SA_RS14110 BioCyc
  • MicrobesOnline: see SA2464

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACCAAATATAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAAT
    CAAACAAAACAAGTATTGATGTTGGGTTATATGAATCAAGAAGCTTTTGATAAAACGATA
    GAAGATGGTGTGGTATGTTTCTATTCGCGGTCGAAACAACGTCTATGGACAAAAGGTGAA
    ACATCTGGTCATACGCAACTTGTTAAAGATATTCATGTAGATTGCGACAATGATACTATT
    TTAATTGATGTCATACCAAATGGACCAACATGTCATACAGGCAGTCAAAGTTGTTTCAAC
    ACAGAAGTTCCATTTTCAGTGCAAACATTAGCGCAGACAGTTCAAGATAGTGCCCAATCC
    AATAATGAAAAGTCATATACAAAATATTTATTAACAGAAGGTATAGAAAAGATTACAAAA
    AAATACGGTGAAGAAGCTTTTGAAGTCGTAATTGAAGCAATTAAAGGTGACAAAAAAGCA
    TTTGTAAGTGAAGTAGCAGATGAACTTTATCATTTATTTGTCTTGATGCATGCGCTAGGC
    GTCGATTTTTCAGAAATTGAGGCGGAATTAGCGCGTAGACATCATAAGCGCAATAACTTT
    AAAGGTGAACGACAAAATATCGAACAGTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS14110 [old locus tag: SA2464 ]
  • symbol: SA_RS14110
  • description: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase
  • length: 210
  • theoretical pI: 5.6078
  • theoretical MW: 23943.9
  • GRAVY: -0.489048

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.4.19?  ExPASy
    Phosphoribosyl-AMP cyclohydrolase 1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide
    EC 3.6.1.31?  ExPASy
    Phosphoribosyl-ATP diphosphatase 1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family phosphoribosyl-ATP diphosphatase (TIGR03188; EC 3.6.1.31; HMM-score: 90.9)
  • TheSEED: see SA2464
  • PFAM:
    no clan defined PRA-CH; Phosphoribosyl-AMP cyclohydrolase (PF01502; HMM-score: 114.7)
    and 5 more
    MazG (CL0231) PRA-PH; Phosphoribosyl-ATP pyrophosphohydrolase (PF01503; HMM-score: 51.3)
    MazG-like; MazG-like family (PF12643; HMM-score: 18.7)
    MazG; MazG nucleotide pyrophosphohydrolase domain (PF03819; HMM-score: 15.1)
    Beta_propeller (CL0186) BBS2_Mid; Ciliary BBSome complex subunit 2, middle region (PF14783; HMM-score: 14.7)
    no clan defined MnmE_helical; MnmE helical domain (PF12631; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003913
    • TAT(Tat/SPI): 0.000188
    • LIPO(Sec/SPII): 0.000404
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGVVCFYSRSKQRLWTKGETSGHTQLVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTEVPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKAFVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY, HisR* see SA2464

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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