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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1195 [new locus tag: SACOL_RS06120 ]
  • pan locus tag?: SAUPAN003452000
  • symbol: mraY
  • pan gene symbol?: mraY
  • synonym:
  • product: phospho-N-acetylmuramoyl-pentapeptide- transferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1195 [new locus tag: SACOL_RS06120 ]
  • symbol: mraY
  • product: phospho-N-acetylmuramoyl-pentapeptide- transferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1200166..1201131
  • length: 966
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTTTTGTATATGCGTTATTAGCGCTAGTGATTACATTTGTTTTGGTACCTGTTTTA
    ATACCTACATTAAAAAGGATGAAATTTGGTCAAAGTATTCGAGAAGAAGGTCCACAAAGC
    CATATGAAGAAGACTGGTACACCAACGATGGGTGGACTAACATTTCTATTAAGTATTGTG
    ATAACGTCTTTGGTGGCTATTATATTTGTAGATCAAGCTAATCCAATCATACTGTTATTA
    TTTGTGACGATTGGTTTTGGGTTAATTGGTTTTATAGATGATTATATTATTGTTGTTAAA
    AAGAATAACCAAGGTTTAACAAGTAAACAGAAGTTTTTGGCGCAAATTGGTATTGCGATT
    ATATTCTTTGTTTTAAGTAATGTGTTTCATTTGGTGAATTTTTCTACGAGCATACATATT
    CCATTTACGAATGTAGCAATCCCACTATCATTTGCATATGTTATTTTCATTGTTTTTTGG
    CAAGTAGGTTTTTCTAATGCAGTAAATTTAACAGATGGTTTAGATGGATTAGCAACTGGA
    CTGTCAATTATCGGATTTACAATGTATGCCATCATGAGCTTTGTGTTAGGAGAAACGGCA
    ATTGGTATTTTCTGTATCATTATGTTGTTTGCACTTTTAGGATTTTTACCATATAACATT
    AACCCTGCTAAAGTGTTTATGGGAGATACAGGTAGCTTAGCTTTAGGTGGTATATTTGCT
    ACCATTTCAATCATGCTTAATCAGGAATTATCATTAATTTTTATAGGTTTAGTATTCGTA
    ATTGAAACATTATCTGTTATGTTACAAGTCGCTAGCTTTAAATTGACTGGAAAGCGTATA
    TTTAAAATGAGTCCGATTCATCATCATTTTGAATTGATAGGATGGAGCGAATGGAAAGTA
    GTTACAGTATTTTGGGCTGTTGGTCTGATTTCAGGTTTAATCGGTTTATGGATTGGAGTG
    CATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    966

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1195 [new locus tag: SACOL_RS06120 ]
  • symbol: MraY
  • description: phospho-N-acetylmuramoyl-pentapeptide- transferase
  • length: 321
  • theoretical pI: 9.37663
  • theoretical MW: 35232.2
  • GRAVY: 1.09564

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.8.13?  ExPASy
    Phospho-N-acetylmuramoyl-pentapeptide-transferase UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
  • ⊞TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan phospho-N-acetylmuramoyl-pentapeptide-transferase (TIGR00445; EC 2.7.8.13; HMM-score: 340.8)
    and 1 more
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase (TIGR02380; EC 2.7.8.33; HMM-score: 46)
  • TheSEED  :
    • Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)
  • ⊞PFAM:
    no clan defined Glycos_transf_4; Glycosyl transferase family 4 (PF00953; HMM-score: 138.9)
    and 2 more
    MraY_sig1; Phospho-N-acetylmuramoyl-pentapeptide-transferase signature 1 (PF10555; HMM-score: 14.8)
    DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0001
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.030264
    • TAT(Tat/SPI): 0.000548
    • LIPO(Sec/SPII): 0.027617
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKKTGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKNNQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFWQVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPYNINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLTGKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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