PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL2598 [new locus tag: SACOL_RS13600 ]
pan locus tag^?: SAUPAN006259000
symbol: SACOL2598
pan gene symbol^?: —
• synonym:
• product: cobalamin synthesis protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL2598 [new locus tag: SACOL_RS13600 ]
• symbol: SACOL2598
• product: cobalamin synthesis protein
• replicon: chromosome
• strand: -
• coordinates: 2658416..2659306
• length: 891
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3237185 NCBI
• RefSeq: YP_187389 NCBI
• BioCyc: see SACOL_RS13600
• MicrobesOnline: 914068 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAATAGTTATTATAGGTGGGTTTTTAGGTGGCGGTAAAACGACTGTCTTAAATCAT
  TTGCTCGCTGAATCATTAAAGGAATCGCTGAAACCAGCAGTCATCATGAATGAATTTGGG
  AAAATGAGTGTTGATGGTGCCTTAGTATCTGAAGACATACCTTTAAGTGAACTGACAGAG
  GGGTGTATCTGTTGTGCAATGAAAGCAGATGTATCAGAACAGTTACATCAATTATATTTA
  AAAGAGCAACCAGACATTGTATTTATTGAATGTAGTGGGATTGCAGAACCGGTCTCTGTC
  TTAGATGCTTGTTTAACGCCTATTTTAGCTCCGTTTACAACAATTACACATATGATTGGT
  GTAATAGACGCAAGCATGTATAAACACATTAAATCATTCCCTAAAGACATCCAAGGCTTA
  TTTTATGAGCAATTAGCATATTGTTCTGTCTTATTTGTTAATAAAATAGATTCAGCAGAT
  GTTGAAACAACGAGCAAACTATTGAAAGATTTAGAAGTTATTAACCCAGAGGCCGATATA
  CAAGTCGGTATGCATGGCAGCGTCACTTTGCCAATATCAGTTAGACAAATGACAGCAACT
  TCTGACAATAAACATAAGTCTTTACATCAAATGATTAATCATCAATTTGTGCAATCACCA
  GTCAAATGTACTAAAGCAGAGTTTATAAAACGTTTAGCATGCCTTCCGTCTCATATTTAT
  AGGTTGAAAGGGTTTATGACATTTGAAGACACCGCACATACGTATCTCATTCAATTTACA
  CAAGGACAATATGAATTAACACCTGTAGCATTTTCAAAAAAAGTGCCAGAATATTTGGTG
  CTTATCGGAAAGGGTATTTCAAAAGAAGACTATCAATGTTTGGAACAGTAG
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⊟Protein

⊟General

• locus tag: SACOL2598 [new locus tag: SACOL_RS13600 ]
• symbol: SACOL2598
• description: cobalamin synthesis protein
• length: 296
• theoretical pI: 5.98589
• theoretical MW: 32835.1
• GRAVY: 0.117568

⊟Function

• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 83.2)
  and 2 more
  Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 24.8)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 17.4)
• TheSEED  :
  • Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family
  COG0523-Histidine  Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family
• ⊞PFAM:
  P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 172.4)
  and 6 more
  CobW_C (CL0832) CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 40.6)
  P-loop_NTPase (CL0023) Arf; ADP-ribosylation factor family (PF00025; HMM-score: 21)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 14.3)
  nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 14.2)
  AAA_22; AAA domain (PF13401; HMM-score: 13)
  AAA_28; AAA domain (PF13521; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.971
  • Cytoplasmic Membrane Score: 0.0287
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0003
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.048081
  • TAT(Tat/SPI): 0.000366
  • LIPO(Sec/SPII): 0.002087
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57652319 NCBI
• RefSeq: YP_187389 NCBI
• UniProt: A0A0H2WZU8 UniProt

⊟Protein sequence

• MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSVDGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLDACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSADVETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQSPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVPEYLVLIGKGISKEDYQCLEQ

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞regulator: Zur* (repression) regulon

  Zur*

  (TF)

  important in Zinc homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications