PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_2179 [new locus tag: NWMN_RS12590 ]
pan locus tag^?: SAUPAN005740000
symbol: modA
pan gene symbol^?: modA
• synonym:
• product: molybdenum ABC transporter periplasmic molybdate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2179 [new locus tag: NWMN_RS12590 ]
• symbol: modA
• product: molybdenum ABC transporter periplasmic molybdate-binding protein
• replicon: chromosome
• strand: -
• coordinates: 2401421..2402203
• length: 783
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332157 NCBI
• RefSeq: YP_001333213 NCBI
• BioCyc:
• MicrobesOnline: 3707781 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAATTGAAACGTTTATTTGCTGTTGTGATTGCAATGCTTTTAGTATTAGCTGGTTGC
  TCTAATTCTAACGATAATAATGAAAGTAAAAAAGATGACGCAGACAATGGTAAGAAACAA
  GAGATTCAAGTTGCAGCGGCAGCAAGTTTAACAGATGTAACCAAGAAATTAGCTTCAGAA
  TTTAAAAAAGAGCATAAAAATGCTGATATTAAATTTAACTATGGTGGATCAGGGGCATTA
  AGAAAACAAATTGAATCAGGCGCACCTGTTGACGTATTTATGTCTGCAAATACTAAAGAT
  GTAGATGCATTAAAAGACAAGAATAAAGCGCATGATACATATAAATATGCGAAAAATAGT
  CTAGTATTAATTGGTGATAAAGATTCAAATTACACTTCAGTAAAAGACTTAAAAGACAAT
  GATAAATTAGCATTAGGTGAAGTGAAAACTGTACCAGCAGGAAAATATGCGAAACAGTAT
  TTAGATAACAATAACTTATTTAAAGAAGTCGAAAGTAAAATCGTTTATGCTAAAGATGTA
  AAACAAGTATTAAATTATGTTGAAAAGGGTAATGCGAAACAAGGTTTTGTGTATAAAACT
  GACTTATATAAACAAAATAAAAAAATTGATACTGTAAAAGTAATTAAAGAAGTAGAACTT
  AAGAAGCCAATCACATACGAAGCTGGTGCTACATCAGATAGTAAATTAGCAAAAGAGTGG
  ATGGAATTCTTAAAATCAGATAAAGCTAAAGAAATACTAAAAGAATACCACTTTGCAGCA
  TAA
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⊟Protein

⊟General

• locus tag: NWMN_2179 [new locus tag: NWMN_RS12590 ]
• symbol: ModA
• description: molybdenum ABC transporter periplasmic molybdate-binding protein
• length: 260
• theoretical pI: 9.79443
• theoretical MW: 29051
• GRAVY: -0.637308

⊟Function

• ⊞TIGRFAM:
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 184.5)
  and 3 more
  Metabolism Transport and binding proteins Anions phosphate binding protein (TIGR02136; HMM-score: 35)
  tungstate ABC transporter binding protein WtpA (TIGR03730; HMM-score: 34.9)
  Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 15)
• TheSEED  :
  • Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
• ⊞PFAM:
  PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 174.3)
  and 8 more
  SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 73.5)
  Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 28.1)
  PBP_like_2; PBP superfamily domain (PF12849; HMM-score: 21.8)
  no clan defined DUF7537; Family of unknown function (DUF7537) (PF24381; HMM-score: 18.3)
  Pilus_CpaD; Pilus biogenesis CpaD protein (pilus_cpaD) (PF09476; HMM-score: 16)
  DUF6491; Family of unknown function (DUF6491) (PF20101; HMM-score: 14.9)
  DUF1310; Protein of unknown function (DUF1310) (PF07006; HMM-score: 10.9)
  HOATZ-like; Cilia- and flagella-associated protein HOATZ-like (PF17664; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.8428
  • Cell wall & surface Score: 0.0119
  • Extracellular Score: 0.1453
• ⊞LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: -0.33
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: LVLAGCS
• ⊞SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.00027
  • TAT(Tat/SPI): 0.00004
  • LIPO(Sec/SPII): 0.999568
  • Cleavage Site: CS pos: 19-20. LAG-CS. Pr: 1.0000
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222391 NCBI
• RefSeq: YP_001333213 NCBI
• UniProt: A0A0H3K9Z9 UniProt

⊟Protein sequence

• MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQVAAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVDALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQYLDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEVELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: modC < modB < modA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications