PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1014 [new locus tag: NWMN_RS05745 ]
pan locus tag^?: SAUPAN001674000
symbol: NWMN_1014
pan gene symbol^?: —
• synonym:
• product: phage terminase small subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1014 [new locus tag: NWMN_RS05745 ]
• symbol: NWMN_1014
• product: phage terminase small subunit
• replicon: chromosome
• strand: +
• coordinates: 1115489..1115929
• length: 441
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330618 NCBI
• RefSeq: YP_001332048 NCBI
• BioCyc:
• MicrobesOnline: 3706565 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAACGAAAAACAAAAGAGATTCGCAGATGAATATATAATGAATGGATGTAATGGTAAA
  AAAGCAGCAATTACAGCAGGTTATAGTAAGAAAACAGCAGAGTCTTTAGCAAGTCGATTG
  TTAAGAAATGTTAATGTTTCGGAATATATTAAAGAACGATTAGAACAGATACAAGAAGAG
  CGTTTAATGAGTATTACAGAAGCTTTAGCGTTATCTGCTTCTATTGCTAGAGGAGAACCT
  CAAGAGGCTTACAGTAAGAAATATGACCATTTAAACGATGAAGTGGAAAAAGAGGTTACT
  TACACAATCACACCAACTTTTGAAGAGCGTCAGAGATCTATTGACCACATACTAAAAGTA
  CATGGTGCGTATATCGATAAAAAAGAAATTACTCAGAAGAATATTGAGATTAATATTGGT
  GAGTACGATGACGAAAGTTAA
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  441
  

⊟Protein

⊟General

• locus tag: NWMN_1014 [new locus tag: NWMN_RS05745 ]
• symbol: NWMN_1014
• description: phage terminase small subunit
• length: 146
• theoretical pI: 5.13029
• theoretical MW: 16806.7
• GRAVY: -0.819863

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage terminase, small subunit
  Phages, Prophages, Transposable elements Phages, Prophages Phage head and packaging  Phage terminase, small subunit
• ⊞PFAM:
  no clan defined Terminase_2; Terminase small subunit (PF03592; HMM-score: 117.8)
  and 1 more
  DSRM (CL0196) dsrm; Double-stranded RNA binding motif (PF00035; HMM-score: 14.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.964
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0354
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013094
  • TAT(Tat/SPI): 0.000975
  • LIPO(Sec/SPII): 0.007163
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221226 NCBI
• RefSeq: YP_001332048 NCBI
• UniProt: A0A0H3K9D1 UniProt

⊟Protein sequence

• MNEKQKRFADEYIMNGCNGKKAAITAGYSKKTAESLASRLLRNVNVSEYIKERLEQIQEERLMSITEALALSASIARGEPQEAYSKKYDHLNDEVEKEVTYTITPTFEERQRSIDHILKVHGAYIDKKEITQKNIEINIGEYDDES

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1014 > NWMN_1015 > NWMN_1016 > NWMN_1017

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications