PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS09805 [old locus tag: SA1708 ]
• pan locus tag^?: SAUPAN004914000
• symbol: SA_RS09805
• pan gene symbol^?: murT
• synonym:
• product: UDP-N-acetylmuramate--alanine ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS09805 [old locus tag: SA1708 ]
• symbol: SA_RS09805
• product: UDP-N-acetylmuramate--alanine ligase
• replicon: chromosome
• strand: -
• coordinates: 1953797..1955110
• length: 1314
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1953797..1955110) NCBI
• BioCyc: SA_RS09805 BioCyc
• MicrobesOnline: see SA1708

⊟Phenotype

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⊟DNA sequence

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  ATGAGACAGTGGACGGCAATCCATCTAGCGAAATTGGCGCGTAAAGCAAGTAGAGCAGTA
  GGTAAAAGAGGAACAGATTTACCTGGACAAATCGCTAGAAAAGTGGATACAGATGTATTA
  AGAAAATTAGCAGAGCAAGTTGATGATATTGTATTTATCAGTGGAACAAATGGTAAAACA
  ACGACTTCAAACTTAATTGGACATACTTTAAAAGCAAATAATATTCAAATTATACACAAT
  AATGAAGGTGCTAATATGGCTGCAGGTATAACTTCTGCATTCATCATGCAATCAACACCT
  AAGACTAAAATTGCGGTAATCGAAATTGATGAAGGTTCGATTCCACGTGTGTTAAAAGAA
  GTTACACCTTCAATGATGGTATTTACTAATTTCTTTAGAGATCAAATGGATCGCTTCGGT
  GAAATTGATATTATGGTTAATAACATTGCAGAGACAATTAGTAATAAAGGCATCAAATTA
  TTGCTAAATGCTGATGATCCATTTGTGAGTCGTTTGAAAATCGCAAGTGATACGATTGTG
  TACTATGGTATGAAAGCACATGCCCATGAATTTGAACAAAGTACGATGAATGAAAGTAGA
  TATTGTCCAAACTGTGGTCGCTTATTGCAATACGATTATATTCATTATAATCAAATTGGT
  CATTATCACTGTCAGTGTGGTTTCAAACGAGAGCAAGCAAAATATGAAATATCAAGTTTT
  GATGTGGCACCGTTTTTATATTTAAATATCAATGATGAAAAATATGATATGAAAATTGCA
  GGTGACTTTAACGCTTATAACGCGTTAGCAGCATATACTGTTTTAAGAGAGCTAGGGTTA
  AATGAACAAACAATTAAAAATGGCTTTGAAACGTATACATCAGACAATGGTCGTATGCAG
  TACTTTAAAAAAGAACGAAAAGAAGCGATGATCAATTTAGCTAAAAATCCTGCAGGAATG
  AATGCAAGTTTATCAGTTGGTGAACAATTAGAAGGCGAAAAAGTGTATGTTATTTCGCTA
  AATGATAACGCTGCAGATGGTCGAGATACTTCATGGATTTATGATGCAGATTTTGAAAAA
  TTATCTAAGCAACAAATTGAAGCTATCATCGTGACAGGTACACGAGCAGAAGAACTTCAA
  TTGCGATTGAAGTTAGCAGAGGTTGAAGTACCAATTATAGTTGAGCGTGATATTTATAAA
  GCAACGGCAAAGACTATGGATTATAAAGGTTTCACAGTTGCAATACCAAACTATACATCA
  TTAGCGCCTATGCTTGAACAATTAAACCGTTCGTTTGAAGGAGGTCAATCATAA
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⊟Protein

⊟General

• locus tag: SA_RS09805 [old locus tag: SA1708 ]
• symbol: SA_RS09805
• description: UDP-N-acetylmuramate--alanine ligase
• length: 437
• theoretical pI: 6.52509
• theoretical MW: 49191.7
• GRAVY: -0.350114

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 64.4)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 61.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 58.6)
  and 6 more
  Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 46.4)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 46.1)
  Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 23.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 22.1)
  HprK-related kinase A (TIGR04352; HMM-score: 16.9)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 14.1)
• TheSEED: see SA1708
• PFAM:
  no clan defined DUF1727; Domain of unknown function (DUF1727) (PF08353; HMM-score: 101.6)
  and 6 more
  Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 68.6)
  Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 15.8)
  RING (CL0229) zf-C3H2C3; Zinc-finger (PF17122; HMM-score: 13.5)
  Zn_Beta_Ribbon (CL0167) zf-RING_7; C4-type zinc ribbon domain (PF02591; HMM-score: 12.8)
  OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 12.8)
  TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.02669
  • TAT(Tat/SPI): 0.001833
  • LIPO(Sec/SPII): 0.004198
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447173087 NCBI
• RefSeq: WP_001250343 NCBI
• UniProt: see SA1708

⊟Protein sequence

• MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDVLRKLAEQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications