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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04175 [old locus tag: SA0734 ]
  • pan locus tag?: SAUPAN002713000
  • symbol: SA_RS04175
  • pan gene symbol?: est
  • synonym:
  • product: carboxylesterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04175 [old locus tag: SA0734 ]
  • symbol: SA_RS04175
  • product: carboxylesterase
  • replicon: chromosome
  • strand: +
  • coordinates: 840074..840814
  • length: 741
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (840074..840814) NCBI
  • BioCyc: SA_RS04175 BioCyc
  • MicrobesOnline: see SA0734

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTA
    TTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAA
    AAGGGATATACTTCGTATGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATA
    CTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTT
    GAACAAGGTTATGATGAAATTGTTGTTGCTGGTCTATCATTAGGTGGGGATTTTGCTTTA
    AAATTAAGCTTAAATAGAGATGTAAAGGGTATTGTAACGATGTGTGCACCAATGGGTGGC
    AAAACTGAAGGTGCCATTTATGAAGGCTTTTTAGAGTATGCACGTAATTTTAAAAAGTAT
    GAAGGTAAAAATCAAGAGACTATTGATAATGAAATGGATCATTTTAAACCAACTGAAACT
    TTAAAAGAACTAAGTGAAGCATTAGATACGATTAAAGATCAAGTTGATGAGGTATTTGAC
    CCAATTTTAGTGATTCAAGTAGAAAACGACAATATGATTGATCCACAATCCGCAAATTAT
    ATATATGACCATGTAGATTCTGATGACAAAGACATCAAGTGGTATAGTCAATCAGGACAT
    GTTATTACGATTGATAAAGAAAAAGAACAAGTATTTGAAGATATTTATCAATTTTTAGAG
    TCATTAGACTGGTCAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    741

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS04175 [old locus tag: SA0734 ]
  • symbol: SA_RS04175
  • description: carboxylesterase
  • length: 246
  • theoretical pI: 4.28248
  • theoretical MW: 28155.3
  • GRAVY: -0.506911

⊟Function[edit | edit source]

  • TIGRFAM:
    Plasmodium subtelomeric family (TIGR01607; HMM-score: 26.9)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 22.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 22.4)
    and 1 more
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 18.9)
  • TheSEED: see SA0734
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 65.8)
    and 12 more
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 34.2)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 31)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 26.6)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 24.7)
    BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 19.1)
    Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 16.4)
    FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 14.8)
    LisH (CL0561) LisH; LisH (PF08513; HMM-score: 14.4)
    AB_hydrolase (CL0028) DUF2048; Abhydrolase domain containing 18 (PF09752; HMM-score: 13.9)
    no clan defined DUF4611; Domain of unknown function (DUF4611) (PF15387; HMM-score: 13.8)
    AB_hydrolase (CL0028) Chlorophyllase2; Chlorophyllase enzyme (PF12740; HMM-score: 13.6)
    Chlorophyllase; Chlorophyllase (PF07224; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014187
    • TAT(Tat/SPI): 0.008702
    • LIPO(Sec/SPII): 0.004092
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYTSYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKLSLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKNQETIDNEMDHFKPTETLKELSEALDTIKDQVDEVFDPILVIQVENDNMIDPQSANYIYDHVDSDDKDIKWYSQSGHVITIDKEKEQVFEDIYQFLESLDWSE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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