PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0763 [new locus tag: SAUSA300_RS04115 ]
• pan locus tag^?: SAUPAN002713000
• symbol: est
• pan gene symbol^?: est
• synonym:
• product: carboxylesterase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0763 [new locus tag: SAUSA300_RS04115 ]
• symbol: est
• product: carboxylesterase
• replicon: chromosome
• strand: +
• coordinates: 850737..851477
• length: 741
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913000 NCBI
• RefSeq: YP_493464 NCBI
• BioCyc: see SAUSA300_RS04115
• MicrobesOnline: 1292278 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTA
  CTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAG
  AAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATA
  CTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTT
  GAACAAGGTTATGATGAAATTGTTGTTGCTGGTCTATCATTAGGTGGGGATTTTGCTTTA
  AAATTAAGCTTAAATAGAGATGTAAAGGGTATTGTAACGATGTGTGCTCCTATGGGTGGC
  AAAACTGAAGGTGCCATTTATGAAGGCTTTTTAGAATATGCACGCAATTTTAAAAAGTAT
  GAAGGTAAAGATCAAGAGACTATTGATAATGAAATGGATCATTTTAAACCAACTGAAACT
  TTAAAAGAACTAAGTGAAGCATTAGATACGATTAAAGAGCAAGTTGATGAAGTGTTGGAT
  CCTATTTTAGTGATTCAAGCAGAAAACGACAATATGATTGATCCACAATCCGCAAATTAT
  ATATATGACCATGTAGATTCTGATGACAAAAATATCAAGTGGTACAGTGAATCTGGACAT
  GTTATTACGATTGATAAAGAGAAAGAACAAGTATTTGAAGATATTTATCAATTTTTAGAG
  TCATTAGACTGGTCAGAATAA
  60
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  741
  

⊟Protein

⊟General

• locus tag: SAUSA300_0763 [new locus tag: SAUSA300_RS04115 ]
• symbol: Est
• description: carboxylesterase
• length: 246
• theoretical pI: 4.25813
• theoretical MW: 28094.2
• GRAVY: -0.512602

⊟Function

• reaction:
  EC 3.1.1.1?  ExPASy
  Carboxylesterase A carboxylic ester + H₂O = an alcohol + a carboxylate
• TIGRFAM:
  Plasmodium subtelomeric family (TIGR01607; HMM-score: 29.2)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 25.1)
  and 3 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 22.3)
  Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 20.3)
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 11)
• TheSEED  :
  • Carboxylesterase (EC 3.1.1.1)
• PFAM:
  AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 69.8)
  and 13 more
  Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 37.5)
  DLH; Dienelactone hydrolase family (PF01738; HMM-score: 35.9)
  Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 30.3)
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 25.3)
  BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 22.8)
  Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 19.2)
  DUF2048; Abhydrolase domain containing 18 (PF09752; HMM-score: 18.2)
  PhoPQ_related; PhoPQ-activated pathogenicity-related protein (PF10142; HMM-score: 17.7)
  FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 16.4)
  no clan defined DUF4611; Domain of unknown function (DUF4611) (PF15387; HMM-score: 15.5)
  LisH (CL0561) LisH; LisH (PF08513; HMM-score: 14.4)
  AB_hydrolase (CL0028) Chlorophyllase2; Chlorophyllase enzyme (PF12740; HMM-score: 13.8)
  Chlorophyllase; Chlorophyllase (PF07224; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014415
  • TAT(Tat/SPI): 0.008541
  • LIPO(Sec/SPII): 0.004087
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161909 NCBI
• RefSeq: YP_493464 NCBI
• UniProt: A0A0H2XJL0 UniProt

⊟Protein sequence

• MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYTSYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKLSLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTETLKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSESGHVITIDKEKEQVFEDIYQFLESLDWSE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: est > rnr > smpB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications