From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00802
  • pan locus tag?: SAUPAN002713000
  • symbol: SAOUHSC_00802
  • pan gene symbol?: est
  • synonym:
  • product: carboxylesterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00802
  • symbol: SAOUHSC_00802
  • product: carboxylesterase
  • replicon: chromosome
  • strand: +
  • coordinates: 784575..785315
  • length: 741
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTA
    CTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAG
    AAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATA
    CTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTT
    GAACAAGGTTATGATGAAATTGTTGTTGCTGGTCTATCATTAGGTGGGGATTTTGCTTTA
    AAATTAAGCTTAAATAGAGATGTAAAGGGTATTGTAACGATGTGTGCTCCTATGGGTGGC
    AAAACTGAAGGTGCCATTTATGAAGGCTTTTTAGAATATGCACGCAATTTTAAAAAGTAT
    GAAGGTAAAGATCAAGAGACTATTGATAATGAAATGGATCATTTTAAACCAACTGAAACT
    TTAAAAGAACTAAGTGAAGCATTAGATACGATTAAAGAGCAAGTTGATGAAGTGTTGGAT
    CCTATTTTAGTGATTCAAGCAGAAAACGACAATATGATTGATCCACAATCCGCAAATTAT
    ATATATGACCATGTAGATTCTGATGACAAAAATATCAAGTGGTACAGTGAATCTGGACAT
    GTTATTACGATTGATAAAGAGAAAGAACAAGTATTTGAAGATATTTATCAATTTTTAGAG
    TCATTAGACTGGTCAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    741

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00802
  • symbol: SAOUHSC_00802
  • description: carboxylesterase
  • length: 246
  • theoretical pI: 4.25813
  • theoretical MW: 28094.2
  • GRAVY: -0.512602

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.1.1?  ExPASy
    Carboxylesterase A carboxylic ester + H2O = an alcohol + a carboxylate
  • TIGRFAM:
    Plasmodium subtelomeric family (TIGR01607; HMM-score: 29.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 25.1)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 22.3)
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 20.3)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 11)
  • TheSEED  :
    • Carboxylesterase (EC 3.1.1.1)
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 69.8)
    and 13 more
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 37.5)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 35.9)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 30.3)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 25.3)
    BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 22.8)
    Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 19.2)
    DUF2048; Abhydrolase domain containing 18 (PF09752; HMM-score: 18.2)
    PhoPQ_related; PhoPQ-activated pathogenicity-related protein (PF10142; HMM-score: 17.7)
    FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 16.4)
    no clan defined DUF4611; Domain of unknown function (DUF4611) (PF15387; HMM-score: 15.5)
    LisH (CL0561) LisH; LisH (PF08513; HMM-score: 14.4)
    AB_hydrolase (CL0028) Chlorophyllase2; Chlorophyllase enzyme (PF12740; HMM-score: 13.8)
    Chlorophyllase; Chlorophyllase (PF07224; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014415
    • TAT(Tat/SPI): 0.008541
    • LIPO(Sec/SPII): 0.004087
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYTSYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKLSLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTETLKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSESGHVITIDKEKEQVFEDIYQFLESLDWSE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_00802&oldid=67405"