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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03850 [old locus tag: SA0676 ]
  • pan locus tag?: SAUPAN002608000
  • symbol: SA_RS03850
  • pan gene symbol?: recQ1
  • synonym:
  • product: DNA helicase RecQ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03850 [old locus tag: SA0676 ]
  • symbol: SA_RS03850
  • product: DNA helicase RecQ
  • replicon: chromosome
  • strand: +
  • coordinates: 770345..772126
  • length: 1782
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (770345..772126) NCBI
  • BioCyc: SA_RS03850 BioCyc
  • MicrobesOnline: see SA0676

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCAACAAACATTATCGCATTACTTTGGGTATGAAACATTTCGACCAGGACAAGAA
    GAAATTATTAGCAAAGTATTAGACCATCGTAATGTGCTTGGTGTCTTACCAACTGGTGGA
    GGTAAGTCTATATGCTATCAAGTACCAGGTTTATTGTTAGGTGGTACAACAATTGTAATA
    AGTCCACTAATATCATTAATGAAAGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAA
    GCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAACAACGTATTGAAAAAGCATTA
    TCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTT
    TTAAATATGCTTCAGCGTATAAAGATTCACTTAGTCGCGTTTGATGAAGCGCATTGTATT
    TCTAAATGGGGTCATGATTTCAGGCCGAGTTACCAAAATGTTATTTCAAAAGTATTTACG
    TTACCTCAAGATTTTACAATAATAGCGTTGACAGCAACTGCCACGGTTGAAGTACAGCAA
    GATATTAGAGAAAAGTTAAATATCGCTCAAACTGATCAAATTAAAACGAGTACTAAGCGT
    AGAAACTTAATTTTTAAAGTAAATCCTACTTATCAACGTCAAAAATTTATATTGGATTAT
    ATTAAAACACATGATGAAGATGCAGGTATTATTTATTGTTCTACACGTAAGCAAGTTGAA
    GAGCTTCAAGAAGCCTTAGAAAGTCAGAAAATTGAAAGTGTTATATATCATGCAGGTTTG
    AGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTC
    GTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGTTTTGTTATTCAT
    TATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGAC
    GGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTAT
    TTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACT
    AAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTT
    GAACCGAATGAAAAATTAGAAGAATGTGAACAATGTAGTAATTGTGTTCAACAAGATAAA
    TCATATAATATGACACAAGAAGCTAAGATGATTATTAGTTGCATCGCTCGTATGAAACAA
    CAAGAGAGTTATAGTGTTATCATTCAAGTGTTAAGAGGAGAGTCAACAGATTATATTAAG
    TATAAAGGTTATGACCAAATTTCAACCCATGGTTTAATGAAAGGTTACACAACATCAGAA
    TTAAGTCACTTAATAGATGAATTAAGATTCAAAGGGTTCTTAAATGAAAATGACGAAATA
    TTAATGTGTGATACTTCAATTAAAAAATTACTCAGTAATGAAGTAGAAGTATTCACAACA
    CCATTTAAGCAAAAAGCGACTGAAAAAGTATTTATAAATACGGTTGAAGGGGTTGACCGA
    GTATTATTCAGTCAGTTGGTAGAAGTTCGTAAAAAGTTAAGTGACAAATTAACGATAGCA
    CCTGTAAGTATATTTTCTGATTACACGTTGGAGGAATTTGCTAAACGTAAGCCTGCTTCG
    AAACAAGATATGATTAATATTGATGGCGTAGGTAGTTACAAATTAAAACATTATTGTCCA
    GCATTTTTAGAAACGATTCAAAATTATAAAGCCAAAGTATAG
    60
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    180
    240
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    360
    420
    480
    540
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    720
    780
    840
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    960
    1020
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    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1782

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03850 [old locus tag: SA0676 ]
  • symbol: SA_RS03850
  • description: DNA helicase RecQ
  • length: 593
  • theoretical pI: 6.83017
  • theoretical MW: 68169.7
  • GRAVY: -0.376223

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 902.3)
    and 4 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 455.6)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 52.9)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 46)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 35.1)
  • TheSEED: see SA0676
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 93.7)
    HTH (CL0123) RQC; RQC domain (PF09382; HMM-score: 77)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 75.2)
    and 3 more
    HRDC-like (CL0426) HRDC; HRDC domain (PF00570; HMM-score: 61.5)
    no clan defined RecQ_Zn_bind; RecQ zinc-binding (PF16124; HMM-score: 42.7)
    DUF1033; Protein of unknown function (DUF1033) (PF06279; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.039348
    • TAT(Tat/SPI): 0.000613
    • LIPO(Sec/SPII): 0.001449
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSICYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSNGEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFTLPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFILDYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDRVKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERDINLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQCSNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTHGLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATEKVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMINIDGVGSYKLKHYCPAFLETIQNYKAKV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS02020single-stranded DNA-binding protein  [1] (data from MRSA252)
    SA_RS02145IMP dehydrogenase  [1] (data from MRSA252)
    SA_RS0292050S ribosomal protein L7/L12  [1] (data from MRSA252)
    SA_RS02930DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    SA_RS02935DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    SA_RS02955elongation factor G  [1] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [1] (data from MRSA252)
    SA_RS07385DNA-binding protein HU  [1] (data from MRSA252)
    SA_RS0867530S ribosomal protein S4  [1] (data from MRSA252)
    SA_RS11635DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [1] (data from MRSA252)
    SA_RS13915ornithine carbamoyltransferase  [1] (data from MRSA252)
    SA_RS13920arginine deiminase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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