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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS03065 [old locus tag: SA0527 ]
- pan locus tag?: SAUPAN002346000
- symbol: SA_RS03065
- pan gene symbol?: nagB
- synonym:
- product: glucosamine-6-phosphate deaminase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTA
TATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATG
ACAGATTTGTATGAACAACTTGTTAAGTTATTAAATAAAAATCAGTTAAACGTAGACAAT
GTATCCACGTTTAATTTAGACGAATATGTAGGTTTAACCGCATCACATCCGCAAAGTTAT
CACTATTATATGGATGACATGCTTTTCAAACAATATCCTTATTTTAATAGAAAGAACATT
CATATTCCAAATGGAGATGCCGATGATATGAATGCGGAAGCGTCAAAATATAATGACGTT
TTAGAACAACAAGGTCAACGTGATATTCAAATTTTAGGTATTGGTGAAAATGGTCATATT
GGATTTAATGAACCTGGTACGCCGTTTGATAGCGTTACTCATATCGTTGATTTGACTGAA
AGTACTATTAAGGCTAATAGTCGATATTTTAAAAACGAAGATGATGTTCCAAAGCAAGCC
ATTTCGATGGGACTTGCTAATATTCTTCAAGCCAAACGTATCATTTTACTCGCATTTGGT
GAAAAGAAACGTGCTGCTATTACACATTTATTAAATCAGGAAATTTCTGTTGATGTTCCA
GCCACATTACTTCACAAACACCCGAATGTTGAGATATATTTAGACGACGAAGCTTGCCCG
AAAAATGTTGCGAAAATTCATGTCGATGAAATGGATTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS03065 [old locus tag: SA0527 ]
- symbol: SA_RS03065
- description: glucosamine-6-phosphate deaminase
- length: 252
- theoretical pI: 5.53277
- theoretical MW: 28467.1
- GRAVY: -0.41627
⊟Function[edit | edit source]
- reaction: EC 3.5.99.6? ExPASyGlucosamine-6-phosphate deaminase Alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3
- TIGRFAM: Central intermediary metabolism Amino sugars glucosamine-6-phosphate deaminase (TIGR00502; EC 3.5.99.6; HMM-score: 245.9)and 2 moreEnergy metabolism Pentose phosphate pathway 6-phosphogluconolactonase (TIGR01198; EC 3.1.1.31; HMM-score: 64.9)Energy metabolism Pentose phosphate pathway ribose 5-phosphate isomerase A (TIGR00021; EC 5.3.1.6; HMM-score: 11.8)
- TheSEED: see SA0527
- PFAM: ISOCOT_Fold (CL0246) Glucosamine_iso; Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase (PF01182; HMM-score: 71.9)and 1 moreSugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 17.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.019992
- TAT(Tat/SPI): 0.000322
- LIPO(Sec/SPII): 0.008769
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLYEQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHIPNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTESTIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVDVPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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