PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0527 [new locus tag: SA_RS03065 ]
• pan locus tag^?: SAUPAN002346000
• symbol: nagB
• pan gene symbol^?: nagB
• synonym:
• product: glucosamine-6-phosphate isomerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0527 [new locus tag: SA_RS03065 ]
• symbol: nagB
• product: glucosamine-6-phosphate isomerase
• replicon: chromosome
• strand: +
• coordinates: 622117..622875
• length: 759
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123332 NCBI
• RefSeq: NP_373780 NCBI
• BioCyc: see SA_RS03065
• MicrobesOnline: 102806 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAGTATTAAACTTAGGATCGAAAAAACAAGCATCATTCTATGTTGCATGTGAGTTA
  TATAAAGAGATGGCATTTAATCAGCACTGTAAACTAGGTTTAGCAACTGGTGGTACAATG
  ACAGATTTGTATGAACAACTTGTTAAGTTATTAAATAAAAATCAGTTAAACGTAGACAAT
  GTATCCACGTTTAATTTAGACGAATATGTAGGTTTAACCGCATCACATCCGCAAAGTTAT
  CACTATTATATGGATGACATGCTTTTCAAACAATATCCTTATTTTAATAGAAAGAACATT
  CATATTCCAAATGGAGATGCCGATGATATGAATGCGGAAGCGTCAAAATATAATGACGTT
  TTAGAACAACAAGGTCAACGTGATATTCAAATTTTAGGTATTGGTGAAAATGGTCATATT
  GGATTTAATGAACCTGGTACGCCGTTTGATAGCGTTACTCATATCGTTGATTTGACTGAA
  AGTACTATTAAGGCTAATAGTCGATATTTTAAAAACGAAGATGATGTTCCAAAGCAAGCC
  ATTTCGATGGGACTTGCTAATATTCTTCAAGCCAAACGTATCATTTTACTCGCATTTGGT
  GAAAAGAAACGTGCTGCTATTACACATTTATTAAATCAGGAAATTTCTGTTGATGTTCCA
  GCCACATTACTTCACAAACACCCGAATGTTGAGATATATTTAGACGACGAAGCTTGCCCG
  AAAAATGTTGCGAAAATTCATGTCGATGAAATGGATTGA
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⊟Protein

⊟General

• locus tag: SA0527 [new locus tag: SA_RS03065 ]
• symbol: NagB
• description: glucosamine-6-phosphate isomerase
• length: 252
• theoretical pI: 5.53277
• theoretical MW: 28467.1
• GRAVY: -0.41627

⊟Function

• reaction:
  EC 3.5.99.6?  ExPASy
  Glucosamine-6-phosphate deaminase Alpha-D-glucosamine 6-phosphate + H₂O = D-fructose 6-phosphate + NH₃
• TIGRFAM:
  Metabolism Central intermediary metabolism Amino sugars glucosamine-6-phosphate deaminase (TIGR00502; EC 3.5.99.6; HMM-score: 245.9)
  and 2 more
  Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconolactonase (TIGR01198; EC 3.1.1.31; HMM-score: 64.9)
  Metabolism Energy metabolism Pentose phosphate pathway ribose 5-phosphate isomerase A (TIGR00021; EC 5.3.1.6; HMM-score: 11.8)
• TheSEED  :
  • Glucosamine-6-phosphate deaminase (EC 3.5.99.6)
  Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  Glucosamine-6-phosphate deaminase (EC 3.5.99.6)
  and 3 more
  Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  Glucosamine-6-phosphate deaminase (EC 3.5.99.6)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Glucosamine-6-phosphate deaminase (EC 3.5.99.6)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  Glucosamine-6-phosphate deaminase (EC 3.5.99.6)
• PFAM:
  ISOCOT_Fold (CL0246) Glucosamine_iso; Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase (PF01182; HMM-score: 71.9)
  and 1 more
  Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 17.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.019992
  • TAT(Tat/SPI): 0.000322
  • LIPO(Sec/SPII): 0.008769
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926247 NCBI
• RefSeq: NP_373780 NCBI
• UniProt: P99125 UniProt

⊟Protein sequence

• MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLYEQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHIPNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTESTIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVDVPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: nagB > SA0528 > SA0529

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)