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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS02575 [old locus tag: SA0447 ]
- pan locus tag?: SAUPAN002225000
- symbol: SA_RS02575
- pan gene symbol?: rsmI
- synonym:
- product: rRNA (cytidine-2'-O-)-methyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGGCTGTATTATATTTAGTGGGCACACCAATTGGTAATTTAGCAGATATTACTTATAGA
GCAGTTGATGTATTGAAACGTGTTGATATGATTGCTTGTGAAGACACTAGAGTAACTAGT
AAGCTGTGTAATCATTATGATATTCCAACTCCATTAAAGTCATATCACGAACATAACAAG
GATAAGCAGACTGCTTTTATCATTGAACAGTTAGAATTAGGTCTTGACGTTGCGCTCGTA
TCTGATGCTGGATTGCCCTTAATTAGTGATCCTGGATACGAATTAGTAGTGGCAGCCAGA
GAAGCTAATATTAAAGTAGAGACTGTGCCTGGACCTAATGCTGGGCTGACGGCTTTGATG
GCTAGTGGATTACCTTCATATGTATATACATTTTTAGGATTTTTGCCACGAAAAGAGAAA
GAAAAAAGTGCTGTATTAGAGCAACGTATGCATGAAAATAGCACATTAATTATATACGAA
TCACCGCATCGTGTGACAGATACATTAAAAACAATTGCAAAGATAGATGCAACACGACAA
GTATCACTAGGGCGTGAATTAACTAAGAAGTTCGAACAAATTGTAACTGATGATGTAACA
CAATTACAAGCATTGATTCAGCAAGGCGATGTACCATTGAAAGGCGAATTCGTTATCTTA
ATTGAAGGTGCTAAAGCGAACAATGAGATATCGTGGTTTGATGATTTATCTATCAATGAG
CATGTTGATCATTATATTCAAACTTCACAGATGAAACCAAAACAAGCTATTAAAAAAGTT
GCTGAAGAACGACAACTTAAAACGAATGAAGTATATAATATTTATCATCAAATAAGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS02575 [old locus tag: SA0447 ]
- symbol: SA_RS02575
- description: rRNA (cytidine-2'-O-)-methyltransferase
- length: 279
- theoretical pI: 5.62169
- theoretical MW: 31274.6
- GRAVY: -0.208961
⊟Function[edit | edit source]
- reaction: EC 2.1.1.198? ExPASy16S rRNA (cytidine1402-2'-O)-methyltransferase S-adenosyl-L-methionine + cytidine1402 in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
- TIGRFAM: Protein synthesis tRNA and rRNA base modification 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (TIGR00096; EC 2.1.1.198; HMM-score: 274.2)and 6 moreBiosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-4 C11-methyltransferase (TIGR01465; EC 2.1.1.133; HMM-score: 25.2)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen-III C-methyltransferase (TIGR01469; EC 2.1.1.107; HMM-score: 23.3)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-2 C(20)-methyltransferase (TIGR01467; EC 2.1.1.130; HMM-score: 20.5)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B C17-methyltransferase (TIGR01466; EC 2.1.1.131; HMM-score: 17.7)Biosynthesis of cofactors, prosthetic groups, and carriers Other nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)Central intermediary metabolism Nitrogen fixation nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)
- TheSEED: see SA0447
- PFAM: no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 116.7)and 2 moreHexosaminidase (CL0546) Glycohydro_20b2; beta-acetyl hexosaminidase like (PF14845; HMM-score: 15.4)P-loop_NTPase (CL0023) DNA_pol3_delta; DNA polymerase III, delta subunit (PF06144; HMM-score: 12.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helix: 1
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.025341
- TAT(Tat/SPI): 0.000726
- LIPO(Sec/SPII): 0.004956
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCNHYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREANIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYESPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFVILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYHQIS
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
Mol Microbiol: 2002, 43(6);1387-400
[PubMed:11952893] [WorldCat.org] [DOI] (P p)