PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0531 [new locus tag: SACOL_RS02715 ]
• pan locus tag^?: SAUPAN002225000
• symbol: SACOL0531
• pan gene symbol^?: rsmI
• synonym:
• product: tetrapyrrole methylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0531 [new locus tag: SACOL_RS02715 ]
• symbol: SACOL0531
• product: tetrapyrrole methylase
• replicon: chromosome
• strand: +
• coordinates: 540920..541759
• length: 840
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3237106 NCBI
• RefSeq: YP_185419 NCBI
• BioCyc: see SACOL_RS02715
• MicrobesOnline: 912003 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCTGTATTATATTTAGTGGGCACACCAATTGGTAATTTAGCAGATATTACTTATAGA
  GCAGTTGATGTATTGAAACGTGTTGATATGATTGCTTGTGAAGACACTAGAGTAACTAGT
  AAACTGTGTAATCATTATGATATTCCAACTCCATTAAAGTCATATCACGAACATAACAAG
  GATAAGCAGACTGCTTTTATCATTGAACAGTTAGAATTAGGTCTTGACGTTGCGCTCGTA
  TCTGATGCTGGATTGCCCTTAATTAGTGATCCTGGATACGAATTAGTAGTGGCAGCCAGA
  GAAGCTAATATTAAAGTAGAGACTGTGCCTGGACCTAATGCTGGGCTGACGGCTTTGATG
  GCTAGTGGATTACCTTCATATGTATATACATTTTTAGGATTTTTGCCACGAAAAGAGAAA
  GAAAAAAGTGCTGTATTAGAGCAACGTATGCATGAAAATAGCACATTAATTATATACGAA
  TCACCGCATCGTGTGACAGATACATTAAAAACAATTGCAAAGATAGATGCAACACGACAA
  GTATCACTAGGGCGTGAATTAACTAAGAAGTTCGAACAAATTGTAACTGATGATGTAACA
  CAATTACAAGCATTGATTCAGCAAGGCGATGTACCATTGAAAGGCGAATTCGTTATCTTA
  ATTGAAGGTGCTAAAGCGAACAATGAGATATCGTGGTTTGATGATTTATCTATCAATGAG
  CATGTTGATCATTATATTCAAACTTCACAGATGAAACCAAAACAAGCTATTAAAAAAGTT
  GCTGAAGAACGACAACTTAAAACGAATGAAGTATATAATATTTATCATCAAATAAGTTAA
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⊟Protein

⊟General

• locus tag: SACOL0531 [new locus tag: SACOL_RS02715 ]
• symbol: SACOL0531
• description: tetrapyrrole methylase
• length: 279
• theoretical pI: 5.62169
• theoretical MW: 31274.6
• GRAVY: -0.208961

⊟Function

• reaction:
  EC 2.1.1.198?  ExPASy
  16S rRNA (cytidine¹⁴⁰²-2'-O)-methyltransferase S-adenosyl-L-methionine + cytidine¹⁴⁰² in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine¹⁴⁰² in 16S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (TIGR00096; EC 2.1.1.198; HMM-score: 274.2)
  and 6 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-4 C11-methyltransferase (TIGR01465; EC 2.1.1.133; HMM-score: 25.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen-III C-methyltransferase (TIGR01469; EC 2.1.1.107; HMM-score: 23.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-2 C(20)-methyltransferase (TIGR01467; EC 2.1.1.130; HMM-score: 20.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B C17-methyltransferase (TIGR01466; EC 2.1.1.131; HMM-score: 17.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)
• TheSEED  :
  • 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (EC 2.1.1.198)
  Stress Response Heat shock Heat shock dnaK gene cluster extended  rRNA small subunit methyltransferase I
• PFAM:
  no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 116.7)
  and 2 more
  Hexosaminidase (CL0546) Glycohydro_20b2; beta-acetyl hexosaminidase like (PF14845; HMM-score: 15.4)
  P-loop_NTPase (CL0023) DNA_pol3_delta; DNA polymerase III, delta subunit (PF06144; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025341
  • TAT(Tat/SPI): 0.000726
  • LIPO(Sec/SPII): 0.004956
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57651370 NCBI
• RefSeq: YP_185419 NCBI
• UniProt: A0A0H2WYW2 UniProt

⊟Protein sequence

• MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCNHYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREANIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYESPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFVILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYHQIS

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization: Cytoplasmic
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL0529 > SACOL0530 > SACOL0531

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications