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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01755 [old locus tag: SA0304 ]
  • pan locus tag?: SAUPAN001239000
  • symbol: SA_RS01755
  • pan gene symbol?: nanA
  • synonym:
  • product: N-acetylneuraminate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS01755 [old locus tag: SA0304 ]
  • symbol: SA_RS01755
  • product: N-acetylneuraminate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 359812..360693
  • length: 882
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (359812..360693) NCBI
  • BioCyc: SA_RS01755 BioCyc
  • MicrobesOnline: see SA0304

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
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    481
    541
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    841
    ATGAACAAAGATTTAAAAGGTTTATATGCAGCGTTACTCGTTCCTTTTGATGAAAATGGT
    CAAGTAAATGAACAAGGACTTAAACAAATTGCTCAAAATGCCATTGAAACTGAAGAATTA
    GACGGTCTTTATGTAAATGGTAGCTCAGGCGAAAACTTTTTATTAAATACAGAGCAGAAG
    AAGCAAGTTTTCAAGGTTGCCAAAGAGGCAGTTGGGGATAAAGTGAAATTGATTGCTCAA
    GTAGGTTCGTTAGATTTAAATGAAGCCATTGAACTCGGAAAATATGCCACAGAACTCGGT
    TATGATGCACTTTCTGCCGTAACACCATTCTACTATCCATTTACTTTTGAAGAAATTAGA
    GATTACTATTTCGATATTATCGAAGCAACACAGAACAATATGATTATTTATGCAATACCA
    GATTTAACAGGTGTGAATATTTCTATTGAACAATTCAGCGAGCTATTTAATCACGAAAAA
    ATTGTTGGTGTTAAATATACAGCGCCGAACTTCTTCCTACTCGAACGTATTAGAAAAGCA
    TTCCCAGACAAATTAATCTTATCTGGCTTTGATGAAATGTTAGTTCAAGCTACGATTTCT
    GGCGTAGATGGTGCAATTGGTTCTACATATAACGTTAATGGTCGCCGCGCTAGAAAAATC
    TTCGACTTAGCACGACAAGGTCAAATTCAAGAAGCTTACCAACTACAACATGATTCAAAC
    GACATTATCGAAACAGTGTTATCAATGGGGATTTATCCAACATTGAAAGAAATATTACGT
    CATCGTGACATTGATGCAGGATTACCGAAAAGACCTTTCAAACCTTTTAACGAAGCACAT
    CGACAAACATTAGATCAACTCATTGCAAAATACGATTTATAG
    60
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS01755 [old locus tag: SA0304 ]
  • symbol: SA_RS01755
  • description: N-acetylneuraminate lyase
  • length: 293
  • theoretical pI: 4.64673
  • theoretical MW: 33100.4
  • GRAVY: -0.243003

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.3.3?  ExPASy
    N-acetylneuraminate lyase N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 366.5)
    Metabolism Central intermediary metabolism Amino sugars N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 366.5)
    and 3 more
    Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate synthase (TIGR00674; EC 4.3.3.7; HMM-score: 137.1)
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02313; EC 4.1.2.-; HMM-score: 100.4)
    5-dehydro-4-deoxyglucarate dehydratase (TIGR03249; EC 4.2.1.41; HMM-score: 56.6)
  • TheSEED: see SA0304
  • PFAM:
    TIM_barrel (CL0036) DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 280.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016918
    • TAT(Tat/SPI): 0.000578
    • LIPO(Sec/SPII): 0.002025
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEKIVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA, NanR* see SA0304

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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