PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0304 [new locus tag: SA_RS01755 ]
• pan locus tag^?: SAUPAN001239000
• symbol: nanA
• pan gene symbol^?: nanA
• synonym:
• product: N-acetylneuraminate lyase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0304 [new locus tag: SA_RS01755 ]
• symbol: nanA
• product: N-acetylneuraminate lyase
• replicon: chromosome
• strand: -
• coordinates: 359812..360693
• length: 882
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123083 NCBI
• RefSeq: NP_373550 NCBI
• BioCyc: see SA_RS01755
• MicrobesOnline: 102576 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAACAAAGATTTAAAAGGTTTATATGCAGCGTTACTCGTTCCTTTTGATGAAAATGGT
  CAAGTAAATGAACAAGGACTTAAACAAATTGCTCAAAATGCCATTGAAACTGAAGAATTA
  GACGGTCTTTATGTAAATGGTAGCTCAGGCGAAAACTTTTTATTAAATACAGAGCAGAAG
  AAGCAAGTTTTCAAGGTTGCCAAAGAGGCAGTTGGGGATAAAGTGAAATTGATTGCTCAA
  GTAGGTTCGTTAGATTTAAATGAAGCCATTGAACTCGGAAAATATGCCACAGAACTCGGT
  TATGATGCACTTTCTGCCGTAACACCATTCTACTATCCATTTACTTTTGAAGAAATTAGA
  GATTACTATTTCGATATTATCGAAGCAACACAGAACAATATGATTATTTATGCAATACCA
  GATTTAACAGGTGTGAATATTTCTATTGAACAATTCAGCGAGCTATTTAATCACGAAAAA
  ATTGTTGGTGTTAAATATACAGCGCCGAACTTCTTCCTACTCGAACGTATTAGAAAAGCA
  TTCCCAGACAAATTAATCTTATCTGGCTTTGATGAAATGTTAGTTCAAGCTACGATTTCT
  GGCGTAGATGGTGCAATTGGTTCTACATATAACGTTAATGGTCGCCGCGCTAGAAAAATC
  TTCGACTTAGCACGACAAGGTCAAATTCAAGAAGCTTACCAACTACAACATGATTCAAAC
  GACATTATCGAAACAGTGTTATCAATGGGGATTTATCCAACATTGAAAGAAATATTACGT
  CATCGTGACATTGATGCAGGATTACCGAAAAGACCTTTCAAACCTTTTAACGAAGCACAT
  CGACAAACATTAGATCAACTCATTGCAAAATACGATTTATAG
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⊟Protein

⊟General

• locus tag: SA0304 [new locus tag: SA_RS01755 ]
• symbol: NanA
• description: N-acetylneuraminate lyase
• length: 293
• theoretical pI: 4.64673
• theoretical MW: 33100.4
• GRAVY: -0.243003

⊟Function

• reaction:
  EC 4.1.3.3?  ExPASy
  N-acetylneuraminate lyase N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 366.5)
  Metabolism Central intermediary metabolism Amino sugars N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 366.5)
  and 3 more
  Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate synthase (TIGR00674; EC 4.3.3.7; HMM-score: 137.1)
  2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02313; EC 4.1.2.-; HMM-score: 100.4)
  5-dehydro-4-deoxyglucarate dehydratase (TIGR03249; EC 4.2.1.41; HMM-score: 56.6)
• TheSEED  :
  • N-acetylneuraminate lyase (EC 4.1.3.3)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylneuraminate lyase (EC 4.1.3.3)
• PFAM:
  TIM_barrel (CL0036) DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 280.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016918
  • TAT(Tat/SPI): 0.000578
  • LIPO(Sec/SPII): 0.002025
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926017 NCBI
• RefSeq: NP_373550 NCBI
• UniProt: P99123 UniProt

⊟Protein sequence

• MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEKIVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0303 < nanA

⊟Regulation

• regulators: NanR* (repression) regulon, CcpA regulon

  NanR*	(TF)	important in Sialic acid utilization, N-acetylglucosamine utilization; RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)