PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0315 [new locus tag: SAUSA300_RS01680 ]
• pan locus tag^?: SAUPAN001239000
• symbol: nanA
• pan gene symbol^?: nanA
• synonym:
• product: N-acetylneuraminate lyase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0315 [new locus tag: SAUSA300_RS01680 ]
• symbol: nanA
• product: N-acetylneuraminate lyase
• replicon: chromosome
• strand: -
• coordinates: 366772..367653
• length: 882
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914699 NCBI
• RefSeq: YP_493029 NCBI
• BioCyc: see SAUSA300_RS01680
• MicrobesOnline: 1291830 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAACAAAGATTTAAAAGGTTTATATGCAGCGTTACTCGTTCCTTTTGATGAAAATGGT
  CAAGTAAATGAACAAGGACTTAAACAAATTGCTCAAAATGCCATTGAAACTGAAGAATTA
  GACGGTCTTTATGTAAATGGTAGCTCAGGCGAAAACTTTTTATTAAATACAGAGCAGAAG
  AAGCAAGTTTTCAAGGTTGCCAAAGAGGCAGTTGGGGATAAAGTGAAATTGATTGCTCAA
  GTAGGTTCGTTAGATTTAAATGAAGCCATTGAACTTGGAAAATATGCCACAGAACTCGGT
  TATGATGCACTTTCTGCCGTAACACCATTCTACTATCCATTTACTTTTGAAGAAATTAGA
  GATTACTATTTCGATATTATCGAAGCAACTCAGAACAATATGATTATTTATGCAATACCA
  GATTTAACAGGTGTGAATATTTCTATTGAACAATTCAGCGAGCTATTTAATCATGAAAAA
  ATTGTTGGTGTTAAATATACAGCGCCAAACTTCTTCCTACTCGAACGTATTAGAAAAGCA
  TTCCCAGACAAATTAATCTTATCTGGCTTTGATGAAATGTTAGTTCAAGCTACGATTTCT
  GGCGTAGATGGTGCAATTGGTTCTACATATAACGTTAATGGTCGCCGTGCAAGAAAAATC
  TTCGACTTAGCACGACAAGGTCAAATTCAAGAAGCTTACCAACTGCAACATGATTCAAAC
  GACATTATCGAAACAGTGTTATCAATGGGGATTTATCCAACATTGAAAGAAATATTACGT
  CATCGTGGCATTGATGCAGGATTACCGAAAAGACCTTTCAAACCTTTTAACGAAGCACAT
  CGACAAACATTAGATCAACTCATTGCAAAATACGATTTATAG
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⊟Protein

• 4AH7
  PDB
• 4AHO
  PDB
• 4AHP
  PDB
• 4AHQ
  PDB
• 4AMA
  PDB
• 5A8G
  PDB
• 5KZD
  PDB
• 5KZE
  PDB
• 5LKY
  PDB

Protein Data Bank: 4AH7

Protein Data Bank: 4AHO

Protein Data Bank: 4AHP

Protein Data Bank: 4AHQ

Protein Data Bank: 4AMA

Protein Data Bank: 5A8G

Protein Data Bank: 5KZD

Protein Data Bank: 5KZE

Protein Data Bank: 5LKY

⊟General

• locus tag: SAUSA300_0315 [new locus tag: SAUSA300_RS01680 ]
• symbol: NanA
• description: N-acetylneuraminate lyase
• length: 293
• theoretical pI: 4.70663
• theoretical MW: 33042.3
• GRAVY: -0.232423

⊟Function

• reaction:
  EC 4.1.3.3?  ExPASy
  N-acetylneuraminate lyase N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 367.8)
  Metabolism Central intermediary metabolism Amino sugars N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 367.8)
  and 3 more
  Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate synthase (TIGR00674; EC 4.3.3.7; HMM-score: 139.2)
  2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02313; EC 4.1.2.-; HMM-score: 103.3)
  5-dehydro-4-deoxyglucarate dehydratase (TIGR03249; EC 4.2.1.41; HMM-score: 56.9)
• TheSEED  :
  • N-acetylneuraminate lyase (EC 4.1.3.3)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylneuraminate lyase (EC 4.1.3.3)
• PFAM:
  TIM_barrel (CL0036) DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 282.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016918
  • TAT(Tat/SPI): 0.000578
  • LIPO(Sec/SPII): 0.002025
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162152 NCBI
• RefSeq: YP_493029 NCBI
• UniProt: Q2FJU9 UniProt

⊟Protein sequence

• MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLYVNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYDALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEKIVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARKIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPFNEAHRQTLDQLIAKYDL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0314 < nanA

⊟Regulation

• regulators: NanR* (repression) regulon, CcpA regulon

  NanR*	(TF)	important in Sialic acid utilization, N-acetylglucosamine utilization; RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications