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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS10205 [old locus tag: SAUSA300_1869 ]
  • pan locus tag?: SAUPAN004903000
  • symbol: SAUSA300_RS10205
  • pan gene symbol?: map
  • synonym:
  • product: methionine aminopeptidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS10205 [old locus tag: SAUSA300_1869 ]
  • symbol: SAUSA300_RS10205
  • product: methionine aminopeptidase
  • replicon: chromosome
  • strand: -
  • coordinates: 2028565..2029323
  • length: 759
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATTGTAAAAACAGAAGAAGAATTACAAGCGTTAAAAGAAATTGGATACATATGCGCT
    AAAGTGCGCAATACAATGCAAGCTGCAACCAAACCAGGTATCACTACGAAAGAGCTTGAT
    AATATTGCGAAAGAGTTATTTGAAGAATACGGTGCTATTTCTGCGCCAATTCATGATGAA
    AATTTTCCTGGTCAAACGTGTATTAGTGTCAATGAAGAGGTGGCACATGGGATTCCAAGT
    AAGCGTGTCATTCGTGAAGGAGATTTAGTAAATATTGATGTATCGGCTTTGAAGAATGGC
    TATTATGCAGATACAGGCATTTCATTTGTCGTTGGAGAATCAGATGATCCAATGAAACAA
    AAAGTATGTGACGTAGCAACGATGGCATTTGAGAATGCAATTGCAAAAGTAAAACCGGGT
    ACTAAGTTAAGTAACATTGGTAAAGCGGTGCATAATACAGCTAGACAAAATGATTTGAAA
    GTCATTAAAAACTTAACAGGTCATGGTGTTGGTTTATCATTACATGAAGCACCAGCACAT
    GTACTTAATTACTTTGATCCAAAAGACAAAACATTATTAACTGAAGGTATGGTATTAGCT
    ATTGAACCGTTTATCTCATCAAATGCATCATTTGTTACAGAAGGTAAAAATGAATGGGCT
    TTTGAAACGAGCGATAAAAGTTTTGTTGCTCAAATTGAGCATACGGTTATCGTGACTAAG
    GATGGTCCGATTTTAACGACAAAGATTGAAGAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    759

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS10205 [old locus tag: SAUSA300_1869 ]
  • symbol: SAUSA300_RS10205
  • description: methionine aminopeptidase
  • length: 252
  • theoretical pI: 4.93689
  • theoretical MW: 27502.1
  • GRAVY: -0.215476

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.11.18?  ExPASy
    Methionyl aminopeptidase Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair methionine aminopeptidase, type I (TIGR00500; EC 3.4.11.18; HMM-score: 282.6)
    and 3 more
    Genetic information processing Protein fate Protein modification and repair methionine aminopeptidase, type II (TIGR00501; EC 3.4.11.18; HMM-score: 110.5)
    Unknown function General DNA-binding protein, 42 kDa (TIGR00495; HMM-score: 53.2)
    ectoine utilization protein EutD (TIGR02993; EC 3.-.-.-; HMM-score: 11.5)
  • TheSEED: see SAUSA300_1869
  • PFAM:
    no clan defined Peptidase_M24; Metallopeptidase family M24 (PF00557; HMM-score: 168.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Co2+, Fe2+, Mn2+, Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011197
    • TAT(Tat/SPI): 0.001706
    • LIPO(Sec/SPII): 0.00293
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAKELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYYADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLKVIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNEWAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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