PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS09470 [old locus tag: SAUSA300_1731 ]
• pan locus tag^?: SAUPAN004503000
• symbol: SAUSA300_RS09470
• pan gene symbol^?: pckA
• synonym:
• product: phosphoenolpyruvate carboxykinase (ATP)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS09470 [old locus tag: SAUSA300_1731 ]
• symbol: SAUSA300_RS09470
• product: phosphoenolpyruvate carboxykinase (ATP)
• replicon: chromosome
• strand: +
• coordinates: 1915442..1917034
• length: 1593
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1915442..1917034) NCBI
• BioCyc: SAUSA300_RS09470 BioCyc
• MicrobesOnline: see SAUSA300_1731

⊟Phenotype

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⊟DNA sequence

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  ATGTCAGTAGACACATACACTGAAACAACTAAAATTGACAAATTACTGAAAAAACCAACG
  TCACATTTTCAACTTTCGACGACACAACTTTATAATAAAATCTTAGACAATAACGAAGGG
  GTATTAACAGAACTTGGTGCTGTTAATGCAAGTACTGGAAAATATACTGGTCGTTCGCCT
  AAAGACAAATTTTTTGTCTCTGAACCTTCATATAGAGATAACATTGATTGGGGAGAAATT
  AATCAACCTATCGATGAAGAAACTTTCTTGAAGTTATACCATAAAGTACTAGACTATTTA
  GATAAAAAAGATGAACTATACGTATTTAAAGGCTACGCTGGTAGCGATAAAGATACAATG
  TTAAAACTTACAGTCATCAATGAATTAGCATGGCATAATTTATTTGCTAAAAATATGTTT
  ATTAGACCTGAATCAAAAGAAGAAGCTACAAAGATTAAACCTAACTTCACTATCGTTTCT
  GCACCACATTTTAAAGCAGATCCAGAAGTTGATGGTACTAAATCTGAAACCTTTGTCATT
  ATTTCATTTAAACACAAAGTCATTTTAATCGGCGGTACTGAATACGCTGGTGAAATGAAA
  AAAGGTATCTTCTCTGTAATGAATTATCTCTTACCGATGCAAGATATTATGAGCATGCAT
  TGCTCAGCAAACGTTGGTGAAAAAGGCGATGTTGCATTATTCTTTGGTCTATCTGGCACT
  GGTAAAACAACCTTATCGGCTGACCCACACCGTAAACTAATCGGTGATGATGAACACGGC
  TGGAATAAAAACGGGGTCTTTAATATCGAAGGTGGCTGCTATGCAAAAGCAATTAATCTT
  TCCAAAGAAAAAGAACCACAGATTTTTGACGCAATCAAATATGGTGCAATTTTAGAGAAC
  ACTGTAGTTGCAGAAGATGGTTCAGTGGACTTTGAAGACAATCGTTATACAGAAAACACG
  CGTGCCGCTTATCCAATTAATCACATTGACAATATTGTAGTACCATCTAAAGCAGCACAT
  CCAAATACAATTATTTTCTTAACTGCGGATGCATTTGGTGTTATTCCACCGATTTCAAAG
  TTAAATAAAGACCAAGCAATGTATCATTTCTTGAGTGGTTTCACTTCTAAATTAGCTGGT
  ACAGAGCGTGGTGTGACAGAACCTGAACCATCATTCTCAACATGTTTCGGAGCACCGTTC
  TTCCCGTTACACCCTACTGTTTACGCTGATCTATTAGGTGAACTTATCGATTTACATGAT
  GTTGATGTTTATCTTGTTAATACTGGATGGACTGGCGGAAAATATGGTGTAGGACGTAGA
  ATCAGCTTACATTACACACGTCAAATGGTAAACCAAGCGATTTCTGGCAAATTGAAAAAT
  GCAGAATATACAAAAGATAGTACGTTTGGTTTAAGCATTCCTGTAGAAATTGAAGATGTA
  CCGAAAACAATTTTAAATCCAATTAATGCTTGGAGCGACAAAGAGAAATATAAAGCACAA
  GCAGAAGATTTAATTCAACGTTTTGAAAAGAACTTCGAAAAATTTGGTGAAAAAGTTGAA
  CATATTGCTGAAAAAGGTAGCTTCAACAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS09470 [old locus tag: SAUSA300_1731 ]
• symbol: SAUSA300_RS09470
• description: phosphoenolpyruvate carboxykinase (ATP)
• length: 530
• theoretical pI: 6.01708
• theoretical MW: 59376.9
• GRAVY: -0.388491

⊟Function

• reaction:
  EC 4.1.1.49?  ExPASy
  Phosphoenolpyruvate carboxykinase (ATP) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO₂
• TIGRFAM:
  Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate carboxykinase (ATP) (TIGR00224; EC 4.1.1.49; HMM-score: 792.5)
  and 1 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 11.4)
• TheSEED: see SAUSA300_1731
• PFAM:
  PEP-carboxyk (CL0374) PEPCK_ATP; Phosphoenolpyruvate carboxykinase (PF01293; HMM-score: 735)
  and 2 more
  PEPCK_C; Phosphoenolpyruvate carboxykinase C-terminal P-loop domain (PF00821; HMM-score: 15.3)
  P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016392
  • TAT(Tat/SPI): 0.000978
  • LIPO(Sec/SPII): 0.000732
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446032051 NCBI
• RefSeq: WP_000109906 NCBI
• UniProt: see SAUSA300_1731

⊟Protein sequence

• MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSAPHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SAUSA300_1731

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications