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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001778
  • pan locus tag?: SAUPAN004503000
  • symbol: pckA
  • pan gene symbol?: pckA
  • synonym:
  • product: phosphoenolpyruvate carboxykinase (ATP)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001778
  • symbol: pckA
  • product: phosphoenolpyruvate carboxykinase (ATP)
  • replicon: chromosome
  • strand: +
  • coordinates: 1830590..1832182
  • length: 1593
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCAGTAGACACATACACTGAAACAACTAAAATTGACAAATTACTGAAAAAACCAACG
    TCACATTTTCAACTTTCGACGACACAACTTTATAATAAAATCTTAGACAATAACGAAGGG
    GCATTAACAGAACTTGGTGCTGTTAATGCAAGTACTGGAAAATATACTGGTCGTTCGCCT
    AAAGACAAATTTTTTGTCTCTGAACCTTCATATAGAGATAACATTGATTGGGGAGAAATT
    AATCAACCTATCGATGAAGAAACTTTCTTGAAGTTATACCATAAAGTACTAGACTATTTA
    GATAAAAAAGATGAACTATACGTATTTAAAGGCTACGCTGGTAGCGATAAAGATACAATG
    TTAAAACTTACAGTCATCAATGAATTAGCATGGCATAATTTATTTGCTAAAAATATGTTT
    ATTAGACCTGAATCAAAAGAAGAAGCTACAAAGATTAAACCTAACTTCACTATCGTTTCT
    GCACCACATTTTAAAGCAAATCCAGAAGTTGATGGTACTAAATCTGAAACCTTTGTCATT
    ATTTCATTTAAACACAAAGTCATTTTAATCGGCGGTACTGAATACGCTGGTGAAATGAAA
    AAAGGTATCTTCTCTGTAATGAATTATCTCTTACCGATGCAAGATATTATGAGCATGCAT
    TGCTCAGCAAACGTTGGTGAAAAAGGCGATGTTGCATTATTCTTTGGTCTATCTGGCACT
    GGTAAAACAACCTTATCGGCTGACCCACACCGTAAACTAATCGGTGATGATGAACACGGC
    TGGAATAAAAACGGGGTCTTTAATATCGAAGGTGGCTGCTATGCAAAAGCAATTAATCTT
    TCCAAAGAAAAAGAACCACAGATTTTTGACGCAATCAAATATGGTGCAATTTTAGAGAAC
    ACTGTAGTTGCAGAAGATGGTTCAGTGGACTTTGAAGACAATCGTTATACAGAAAACACG
    CGTGCCGCTTATCCAATTAATCACATTGACAATATTGTAGTACCATCAAAAGCAGCACAT
    CCAAATACAATTATTTTCTTAACTGCGGATGCATTTGGTGTTATTCCACCGATTTCAAAG
    TTAAATAAAGACCAAGCAATGTATCATTTCTTGAGTGGTTTCACTTCTAAATTAGCTGGT
    ACAGAGCGTGGTGTGACAGAACCTGAACCATCATTCTCAACATGTTTCGGCGCACCGTTC
    TTCCCGTTACACCCTACTGTTTACGCTGATCTATTAGGTGAACTTATCGATTTACATGAT
    GTTGATGTTTATCTTGTTAATACTGGATGGACTGGCGGAAAATATGGTGTAGGGCGTAGA
    ATCAGCTTACATTACACACGTCAAATGGTAAACCAAGCGATTTCTGGCAAATTGAAAAAT
    GCAGAATATACAAAAGATAGTACGTTTGGTTTAAGCATTCCTGTAGAAATTGAAGATGTA
    CCGAAAACAATTTTAAATCCAATTAATGCTTGGAGCGACAAAGAGAAATATAAAGCACAA
    GCAGAAGATTTAATTCAACGTTTTGAAAAGAACTTCGAAAAATTTGGTGAAAAAGTTGAA
    CATATTGCTGAAAAAGGTAGCTTCAACAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1593

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001778
  • symbol: PckA
  • description: phosphoenolpyruvate carboxykinase (ATP)
  • length: 530
  • theoretical pI: 6.13605
  • theoretical MW: 59347.9
  • GRAVY: -0.393019

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.49?  ExPASy
    Phosphoenolpyruvate carboxykinase (ATP) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate carboxykinase (ATP) (TIGR00224; EC 4.1.1.49; HMM-score: 790.5)
    and 1 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 11.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PEP-carboxyk (CL0374) PEPCK_ATP; Phosphoenolpyruvate carboxykinase (PF01293; HMM-score: 742.3)
    and 1 more
    PEPCK_GTP; Phosphoenolpyruvate carboxykinase C-terminal P-loop domain (PF00821; HMM-score: 17.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6106
    • Cytoplasmic Membrane Score: 0.1326
    • Cell wall & surface Score: 0.0012
    • Extracellular Score: 0.2557
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011244
    • TAT(Tat/SPI): 0.00137
    • LIPO(Sec/SPII): 0.0008
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGALTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSAPHFKANPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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