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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1681 [new locus tag: NWMN_RS09430 ]
- pan locus tag?: SAUPAN004503000
- symbol: pckA
- pan gene symbol?: pckA
- synonym:
- product: phosphoenolpyruvate carboxykinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1681 [new locus tag: NWMN_RS09430 ]
- symbol: pckA
- product: phosphoenolpyruvate carboxykinase
- replicon: chromosome
- strand: +
- coordinates: 1875972..1877564
- length: 1593
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5332128 NCBI
- RefSeq: YP_001332715 NCBI
- BioCyc:
- MicrobesOnline: 3707233 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1561ATGTCAGTAGACACATACACTGAAACAACTAAAATTGACAAATTACTGAAAAAACCAACG
TCACATTTTCAACTTTCGACGACACAACTTTATAATAAAATCTTAGACAATAACGAAGGG
GTATTAACAGAACTTGGTGCTGTTAATGCAAGTACTGGAAAATATACTGGTCGTTCGCCT
AAAGACAAATTTTTTGTCTCTGAACCTTCATATAGAGATAACATTGATTGGGGAGAAATT
AATCAACCTATCGATGAAGAAACTTTCTTGAAGTTATACCATAAAGTACTAGACTATTTA
GATAAAAAAGATGAACTATACGTATTTAAAGGCTACGCTGGTAGCGATAAAGATACAATG
TTAAAACTTACAGTCATCAATGAATTAGCATGGCATAATTTATTTGCTAAAAATATGTTT
ATTAGACCTGAATCAAAAGAAGAAGCTACAAAGATTAAACCTAACTTCACTATCGTTTCT
GCACCACATTTTAAAGCAGATCCAGAAGTTGATGGTACTAAATCTGAAACCTTTGTCATT
ATTTCATTTAAACACAAAGTCATTTTAATCGGCGGTACTGAATACGCTGGTGAAATGAAA
AAAGGTATCTTCTCTGTAATGAATTATCTCTTACCGATGCAAGATATTATGAGCATGCAT
TGCTCAGCAAACGTTGGTGAAAAAGGCGATGTTGCATTATTCTTTGGTCTATCTGGCACT
GGTAAAACAACCTTATCGGCTGACCCACACCGTAAACTAATCGGTGATGATGAACACGGC
TGGAATAAAAACGGGGTCTTTAATATCGAAGGTGGCTGCTATGCAAAAGCAATTAATCTT
TCCAAAGAAAAAGAACCACAGATTTTTGACGCAATCAAATATGGTGCAATTTTAGAGAAC
ACTGTAGTTGCAGAAGATGGTTCAGTGGACTTTGAAGACAATCGTTATACAGAAAACACG
CGTGCCGCTTATCCAATTAATCACATTGACAATATTGTAGTACCATCTAAAGCAGCACAT
CCAAATACAATTATTTTCTTAACTGCGGATGCATTTGGTGTTATTCCACCGATTTCAAAG
TTAAATAAAGACCAAGCAATGTATCATTTCTTGAGTGGTTTCACTTCTAAATTAGCTGGT
ACAGAGCGTGGTGTGACAGAACCTGAACCATCATTCTCAACATGTTTCGGAGCACCGTTC
TTCCCGTTACACCCTACTGTTTACGCTGATCTATTAGGTGAACTTATCGATTTACATGAT
GTTGATGTTTATCTTGTTAATACTGGATGGACTGGCGGAAAATATGGTGTAGGACGTAGA
ATCAGCTTACATTACACACGTCAAATGGTAAACCAAGCGATTTCTGGCAAATTGAAAAAT
GCAGAATATACAAAAGATAGTACGTTTGGTTTAAGCATTCCTGTAGAAATTGAAGATGTA
CCGAAAACAATTTTAAATCCAATTAATGCTTGGAGCGACAAAGAGAAATATAAAGCACAA
GCAGAAGATTTAATTCAACGTTTTGAAAAGAACTTCGAAAAATTTGGTGAAAAAGTTGAA
CATATTGCTGAAAAAGGTAGCTTCAACAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1681 [new locus tag: NWMN_RS09430 ]
- symbol: PckA
- description: phosphoenolpyruvate carboxykinase
- length: 530
- theoretical pI: 6.01708
- theoretical MW: 59376.9
- GRAVY: -0.388491
⊟Function[edit | edit source]
- reaction: EC 4.1.1.49? ExPASyPhosphoenolpyruvate carboxykinase (ATP) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2
- TIGRFAM: Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate carboxykinase (ATP) (TIGR00224; EC 4.1.1.49; HMM-score: 792.5)and 1 moreTransport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 11.4)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: PEP-carboxyk (CL0374) PEPCK_ATP; Phosphoenolpyruvate carboxykinase (PF01293; HMM-score: 735)and 2 morePEPCK_C; Phosphoenolpyruvate carboxykinase C-terminal P-loop domain (PF00821; HMM-score: 15.3)P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 13.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.016392
- TAT(Tat/SPI): 0.000978
- LIPO(Sec/SPII): 0.000732
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTELGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDKKDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVSAPHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMSMHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAKAINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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