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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS08660 [old locus tag: SAUSA300_1589 ]
- pan locus tag?: SAUPAN004238000
- symbol: SAUSA300_RS08660
- pan gene symbol?: dtd
- synonym:
- product: D-tyrosyl-tRNA(Tyr) deacylase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS08660 [old locus tag: SAUSA300_1589 ]
- symbol: SAUSA300_RS08660
- product: D-tyrosyl-tRNA(Tyr) deacylase
- replicon: chromosome
- strand: -
- coordinates: 1740482..1740934
- length: 453
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1740482..1740934) NCBI
- BioCyc: SAUSA300_RS08660 BioCyc
- MicrobesOnline: see SAUSA300_1589
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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421ATGAAAGTAGTTGTACAAAGAGTTAAAGAAGCATCGGTGACGAATGATACATTAAATAAT
CAAATCAAAAAAGGATATTGTTTATTAGTCGGTATCGGTCAGAACTCTACAGAGCAAGAT
GCAGATGTAATTGCAAAGAAAATTGCTAATGCAAGATTATTTGAAGATGACAATAATAAA
TTAAACTTTAATATCCAACAAATGAATGGTGAAATACTATCAGTTTCACAATTTACTCTC
TATGCAGATGTAAAAAAAGGTAACCGTCCAGGTTTCTCAAATTCTAAAAATCCTGATCAA
GCGGTAAAAATTTATGAGTATTTTAATGATGCGCTACGAGCGTATGGTCTTACTGTGAAA
ACAGGTGAATTTGGAACACACATGAATGTTAGCATAAATAATGATGGTCCAGTCACTATT
ATTTATGAAAGTCAGGACGGCAAAATTCAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS08660 [old locus tag: SAUSA300_1589 ]
- symbol: SAUSA300_RS08660
- description: D-tyrosyl-tRNA(Tyr) deacylase
- length: 150
- theoretical pI: 7.34423
- theoretical MW: 16696.7
- GRAVY: -0.502667
⊟Function[edit | edit source]
- reaction: EC 3.1.1.96? ExPASyD-aminoacyl-tRNA deacylase A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala)?
- TIGRFAM: Protein synthesis tRNA aminoacylation D-tyrosyl-tRNA(Tyr) deacylase (TIGR00256; EC 3.6.1.-; HMM-score: 175.4)
- TheSEED: see SAUSA300_1589
- PFAM: no clan defined Tyr_Deacylase; D-Tyr-tRNA(Tyr) deacylase (PF02580; HMM-score: 171.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.027251
- TAT(Tat/SPI): 0.000323
- LIPO(Sec/SPII): 0.002547
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446792727 NCBI
- RefSeq: WP_000869983 NCBI
- UniProt: see SAUSA300_1589
⊟Protein sequence[edit | edit source]
- MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVIAKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAVKIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.