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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08230 [old locus tag: SAUSA300_1507 ]
  • pan locus tag?: SAUPAN004126000
  • symbol: SAUSA300_RS08230
  • pan gene symbol?: glk
  • synonym:
  • product: glucokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08230 [old locus tag: SAUSA300_1507 ]
  • symbol: SAUSA300_RS08230
  • product: glucokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1658088..1659074
  • length: 987
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAAAAATTATTTTAGCAGCTGATGTAGGCGGGACGACTTGTAAATTAGGTATTTTC
    ACACCTGAATTAGAACAATTACATAAATGGTCTATTCACACTGATACATCTGATAGTACA
    GGATATACACTTTTGAAAGGAATTTATGATTCGTTTGTTGAAAAAGTAAATGAAAATAAT
    TATAATTTTTCAAATGTACTTGGCGTAGGTATTGGTGTACCAGGTCCTGTTGACTTTGAA
    AAAGGTACAGTAAATGGAGCAGTAAACTTATATTGGCCAGAAAAAGTTAATGTACGTGAG
    ATTTTTGAACAATTCGTTGATTGTCCAGTGTATGTAGATAATGATGCTAACATAGCTGCT
    TTAGGGGAGAAACACAAAGGTGCTGGTGAAGGTGCCGATGATGTTGTTGCCATCACACTT
    GGTACAGGTCTAGGTGGAGGAATTATTTCAAATGGTGAAATCGTACATGGTCATAATGGC
    TCTGGCGCAGAAATAGGTCATTTTAGAGCAGACTTCGATCAACGATTTAAATGTAATTGT
    GGTCGTTCTGGATGTATTGAAACAGTTGCTTCAGCGACAGGCGTTGTTAACTTAGTTAAC
    TTCTACTATCCGAAGTTGACGTTTAGATCTTCTATATTAGAATTGATTAAAGAAAATAAG
    GTTACAGCAAAAGCTGTTTTTGATGCGGCAAAAGCTGGTGACCAATTCTGTATTTTCATT
    ACTGAAAAGGTTGCAAACTATATTGGATATTTATGTAGTATTATTAGTGTTACAAGTAAT
    CCGAAATATATCGTTCTAGGTGGAGGAATGTCTACTGCAGGACCTATTTTAATTGAAAAT
    ATTAAAACAGAATATCATAATTTAACATTTGCACCTGCTCAATTTGAAACTGAAATTGTA
    CAAGCGAAATTAGGTAATGATGCAGGTATTACAGGAGCAGCAGGATTAATCAAGACCTAT
    GTATTAGATAAAGAGGGGGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS08230 [old locus tag: SAUSA300_1507 ]
  • symbol: SAUSA300_RS08230
  • description: glucokinase
  • length: 328
  • theoretical pI: 5.04559
  • theoretical MW: 35077.6
  • GRAVY: 0.0429878

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.2?  ExPASy
    Glucokinase ATP + D-glucose = ADP + D-glucose 6-phosphate
  • TIGRFAM:
    Unknown function General ROK family protein (putative glucokinase) (TIGR00744; HMM-score: 496.8)
  • TheSEED: see SAUSA300_1507
  • PFAM:
    Actin_ATPase (CL0108) ROK; ROK family (PF00480; HMM-score: 221.1)
    and 1 more
    Glucokinase; Glucokinase (PF02685; HMM-score: 25.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009185
    • TAT(Tat/SPI): 0.000323
    • LIPO(Sec/SPII): 0.00625
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTLLKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIFEQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNGSGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKENKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPILIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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