Jump to navigation
Jump to search
NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01646
- pan locus tag?: SAUPAN004126000
- symbol: SAOUHSC_01646
- pan gene symbol?: glk
- synonym:
- product: glucokinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01646
- symbol: SAOUHSC_01646
- product: glucokinase
- replicon: chromosome
- strand: -
- coordinates: 1561291..1562277
- length: 987
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3920098 NCBI
- RefSeq: YP_500158 NCBI
- BioCyc: G1I0R-1531 BioCyc
- MicrobesOnline: 1290072 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961ATGACAAAAATTATTTTAGCAGCTGATGTAGGCGGGACGACTTGTAAATTAGGTATTTTC
ACACCTGAATTAGAACAATTACATAAATGGTCTATTCACACTGATACATCTGATAGTACA
GGATATACACTTTTGAAAGGAATTTATGATTCGTTTGTTGAAAAAGTAAATGAAAATAAT
TATAATTTTTCAAATGTACTTGGCGTAGGTATTGGTGTACCAGGTCCTGTTGACTTTGAA
AAAGGTACAGTAAATGGAGCAGTAAACTTATATTGGCCAGAAAAAGTTAATGTACGTGAG
ATTTTTGAACAATTCGTTGATTGTCCAGTGTATGTAGATAATGATGCTAACATAGCTGCT
TTAGGGGAGAAACACAAAGGTGCTGGTGAAGGTGCCGATGATGTTGTTGCCATCACACTT
GGTACAGGTCTAGGTGGAGGAATTATTTCAAATGGTGAAATCGTACATGGTCATAATGGC
TCTGGCGCAGAAATAGGTCATTTTAGAGCAGACTTCGATCAACGATTTAAATGTAATTGT
GGTCGTTCTGGATGTATTGAAACAGTTGCTTCAGCGACAGGCGTTGTTAACTTAGTTAAC
TTCTACTATCCGAAGTTGACGTTTAGATCTTCTATATTAGAATTGATTAAAGAAAATAAG
GTTACAGCAAAAGCTGTTTTTGATGCGGCAAAAGCTGGTGACCAATTCTGTATTTTCATT
ACTGAAAAGGTTGCAAACTATATTGGATATTTATGTAGTATTATTAGTGTTACAAGTAAT
CCGAAATATATCGTTCTAGGTGGAGGAATGTCTACTGCAGGACCTATTTTAATTGAAAAT
ATTAAAACAGAATATCATAATTTAACATTTGCACCTGCTCAATTTGAAACTGAAATTGTA
CAAGCGAAATTAGGTAATGATGCAGGTATTACAGGAGCAGCAGGATTAATCAAGACCTAT
GTATTAGATAAAGAGGGGGTAAAATAA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
987
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01646
- symbol: SAOUHSC_01646
- description: glucokinase
- length: 328
- theoretical pI: 5.04559
- theoretical MW: 35077.6
- GRAVY: 0.0429878
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009185
- TAT(Tat/SPI): 0.000323
- LIPO(Sec/SPII): 0.00625
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTLLKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIFEQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNGSGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKENKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPILIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01644 < SAOUHSC_01645 < SAOUHSC_01646 < SAOUHSC_01647predicted SigA promoter [3] : SAOUHSC_01644 < SAOUHSC_01645 < SAOUHSC_01646 < SAOUHSC_01647 < SAOUHSC_01649 < SAOUHSC_01650
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)