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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS07385 [old locus tag: SAUSA300_1355 ]
  • pan locus tag?: SAUPAN003924000
  • symbol: SAUSA300_RS07385
  • pan gene symbol?: aroA
  • synonym:
  • product: 3-phosphoshikimate 1-carboxyvinyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS07385 [old locus tag: SAUSA300_1355 ]
  • symbol: SAUSA300_RS07385
  • product: 3-phosphoshikimate 1-carboxyvinyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1521390..1522688
  • length: 1299
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGTAAATGAACAAATCATTGATATTTCAGGTCCGTTAAAGGGCGAAATAGAAGTGCCG
    GGCGATAAGTCAATGACACACCGTGCAATCATGTTGGCGTCGCTAGCTGAAGGTGTATCT
    ACTATATATAAGCCACTACTTGGCGAAGATTGTCGTCGTACGATGGACATTTTCCGACTG
    TTAGGTGTAGAAATCAAAGAAGATGATGAAAAATTAGTTGTGACTTCCCCAGGATATCAA
    TCTTTTAACACGCCACATCAAGTATTGTATACAGGTAATTCTGGTACGACAACACGATTA
    TTGGCAGGTTTGTTAAGTGGTTTAGGTATTGAAAGTGTTTTGTCTGGCGATGTTTCAATT
    GGTAAAAGGCCAATGGATCGTGTCTTGAGACCATTGAAACTTATGGATGCGAATATTGAA
    GGTATTGAAGATAATTATACACCATTAATTATTAAGCCATCTGTCATAAAAGGTATAAAT
    TATCAAATGGAAGTTGCAAGTGCACAAGTAAAAAGTGCCATTTTATTTGCAAGTTTGTTT
    TCTAAGGAACCGACCATCATTAAAGAATTAGATGTAAGTCGAAATCATACTGAGACGATG
    TTCAAACATTTTAATATTCCAATTGAAGCAGAAGGGTTATCAATTAATACAACCCCTGAA
    GCAATTCGATACATTAAACCTGCAGATTTTCATGTTCCTGGCGATATTTCATCTGCAGCG
    TTCTTTATTGTTGCAGCACTTATCACACCAGGAAGTGATGTAACAATTCATAATGTTGGA
    ATCAATCCAACACGTTCAGGTATTATTGATATTGTTGAAAAAATGGGCGGTAATATCCAA
    CTTTTCAATCAAACAACTGGTGCTGAACCTACTGCTTCTATTCGTATTCAATACACACCA
    ATGCTTCAACCAATAACAATCGAAGGAGAATTAGTTCCAAAAGCAATTGATGAACTGCCT
    GTAATAGCATTACTTTGTACACAAGCAGTTGGCACGAGTACAATTAAAGATGCCGAGGAA
    TTAAAAGTAAAAGAAACAAATAGAATTGATACAACGGCTGATATGTTAAACTTGTTAGGG
    TTTGAATTACAACCAACTAATGATGGATTGATTATTCATCCGTCAGAATTTAAAACAAAT
    GCAACAGTTGATAGTTTAACTGATCATCGAATAGGAATGATGCTTGCAGTTGCTTCTCTA
    CTTTCAAGCGAGCCTGTCAAAATCAAACAATTTGATGCTGTAAATGTATCATTTCCAGGA
    TTTTTACCAAAACTAAAGCTTTTAGAAAATGAGGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1299

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS07385 [old locus tag: SAUSA300_1355 ]
  • symbol: SAUSA300_RS07385
  • description: 3-phosphoshikimate 1-carboxyvinyltransferase
  • length: 432
  • theoretical pI: 4.77676
  • theoretical MW: 47002.9
  • GRAVY: 0.0409722

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.19?  ExPASy
    3-phosphoshikimate 1-carboxyvinyltransferase Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 379.1)
    and 1 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 72.7)
  • TheSEED: see SAUSA300_1355
  • PFAM:
    EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 455.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004546
    • TAT(Tat/SPI): 0.001018
    • LIPO(Sec/SPII): 0.000446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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