Jump to navigation
Jump to search
NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1355 [new locus tag: SAUSA300_RS07385 ]
- pan locus tag?: SAUPAN003924000
- symbol: aroA
- pan gene symbol?: aroA
- synonym:
- product: 3-phosphoshikimate 1-carboxyvinyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1355 [new locus tag: SAUSA300_RS07385 ]
- symbol: aroA
- product: 3-phosphoshikimate 1-carboxyvinyltransferase
- replicon: chromosome
- strand: -
- coordinates: 1521390..1522688
- length: 1299
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914972 NCBI
- RefSeq: YP_494052 NCBI
- BioCyc: see SAUSA300_RS07385
- MicrobesOnline: 1292870 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261ATGGTAAATGAACAAATCATTGATATTTCAGGTCCGTTAAAGGGCGAAATAGAAGTGCCG
GGCGATAAGTCAATGACACACCGTGCAATCATGTTGGCGTCGCTAGCTGAAGGTGTATCT
ACTATATATAAGCCACTACTTGGCGAAGATTGTCGTCGTACGATGGACATTTTCCGACTG
TTAGGTGTAGAAATCAAAGAAGATGATGAAAAATTAGTTGTGACTTCCCCAGGATATCAA
TCTTTTAACACGCCACATCAAGTATTGTATACAGGTAATTCTGGTACGACAACACGATTA
TTGGCAGGTTTGTTAAGTGGTTTAGGTATTGAAAGTGTTTTGTCTGGCGATGTTTCAATT
GGTAAAAGGCCAATGGATCGTGTCTTGAGACCATTGAAACTTATGGATGCGAATATTGAA
GGTATTGAAGATAATTATACACCATTAATTATTAAGCCATCTGTCATAAAAGGTATAAAT
TATCAAATGGAAGTTGCAAGTGCACAAGTAAAAAGTGCCATTTTATTTGCAAGTTTGTTT
TCTAAGGAACCGACCATCATTAAAGAATTAGATGTAAGTCGAAATCATACTGAGACGATG
TTCAAACATTTTAATATTCCAATTGAAGCAGAAGGGTTATCAATTAATACAACCCCTGAA
GCAATTCGATACATTAAACCTGCAGATTTTCATGTTCCTGGCGATATTTCATCTGCAGCG
TTCTTTATTGTTGCAGCACTTATCACACCAGGAAGTGATGTAACAATTCATAATGTTGGA
ATCAATCCAACACGTTCAGGTATTATTGATATTGTTGAAAAAATGGGCGGTAATATCCAA
CTTTTCAATCAAACAACTGGTGCTGAACCTACTGCTTCTATTCGTATTCAATACACACCA
ATGCTTCAACCAATAACAATCGAAGGAGAATTAGTTCCAAAAGCAATTGATGAACTGCCT
GTAATAGCATTACTTTGTACACAAGCAGTTGGCACGAGTACAATTAAAGATGCCGAGGAA
TTAAAAGTAAAAGAAACAAATAGAATTGATACAACGGCTGATATGTTAAACTTGTTAGGG
TTTGAATTACAACCAACTAATGATGGATTGATTATTCATCCGTCAGAATTTAAAACAAAT
GCAACAGTTGATAGTTTAACTGATCATCGAATAGGAATGATGCTTGCAGTTGCTTCTCTA
CTTTCAAGCGAGCCTGTCAAAATCAAACAATTTGATGCTGTAAATGTATCATTTCCAGGA
TTTTTACCAAAACTAAAGCTTTTAGAAAATGAGGGATAA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1080
1140
1200
1260
1299
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1355 [new locus tag: SAUSA300_RS07385 ]
- symbol: AroA
- description: 3-phosphoshikimate 1-carboxyvinyltransferase
- length: 432
- theoretical pI: 4.77676
- theoretical MW: 47002.9
- GRAVY: 0.0409722
⊟Function[edit | edit source]
- reaction: EC 2.5.1.19? ExPASy3-phosphoshikimate 1-carboxyvinyltransferase Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
- TIGRFAM: Amino acid biosynthesis Aromatic amino acid family 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 379.1)and 1 moreCell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 72.7)
- TheSEED :
- 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) alpha sub-homology division
Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)and 1 more - PFAM: EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 455.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004546
- TAT(Tat/SPI): 0.001018
- LIPO(Sec/SPII): 0.000446
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: aroA < aroB < aroC
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.