PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS05845 [old locus tag: SAUSA300_1078 ]
• pan locus tag^?: SAUPAN003454000
• symbol: SAUSA300_RS05845
• pan gene symbol^?: divIB
• synonym:
• product: cell division protein DivIB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS05845 [old locus tag: SAUSA300_1078 ]
• symbol: SAUSA300_RS05845
• product: cell division protein DivIB
• replicon: chromosome
• strand: +
• coordinates: 1178679..1179998
• length: 1320
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1178679..1179998) NCBI
• BioCyc: SAUSA300_RS05845 BioCyc
• MicrobesOnline: see SAUSA300_1078

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGATGATAAAACGAAGAACGATCAACAAGAATCAAATGAAGATAAAGATGAATTAGAA
  TTATTTACGAGGAATACATCTAAGAAAAGACGGCAAAGAAAAAGATCAAAGGCTACACAT
  TTTTCTAATCAAAATAAAGATGATACATCTCAACAAGCTGATTTTGATGAAGAAATTTAC
  TTGATAAATAAAGACTTCAAAAAAGAAGAAAGCAATGATAAAAATAATGATTCTGCTTCT
  AGTCATGCGAATGATAATAATATCGATGATTCTACAGACTCTAATATTGAAAATGAGGAT
  TATAGATATAATCAAGAAATTGACGACCAAAATGAATCGAATGTAATTTCAGTCGACAAC
  GAACAACCTCAATCAGCTCCTAAAGAACAAAATAGCGACTCGATTGATGAGGAAACAGTA
  ACGAAAAAAGAACGAAAAAGTAAAGTAACACAATTAAAGCCATTAACACTTGAAGAAAAG
  CGGAAGTTAAGACGTAAGCGACAAAAGCGAATCCAATACAGTGTTATTACAATATTGGTA
  TTGTTGATTGCTGTTATATTAATTTACATGTTTTCACCACTTAGTAAAATTGCGCATGTA
  AATATAAATGGAAATAATCACGTTAGTACTTCAAAGATAAACAAAGTTTTAGGTGTTAAA
  AATGATTCAAGGATGTATACGTTTAGTAAAAAAAATGCTATTAATGATCTCGAAGAGAAT
  CCATTAATCAAAAGTGTTGAGATACACAAGCAATTACCAAACACATTAAACGTAGATATC
  ACAGAAAATGAAATTATTGCTTTAGTGAAATATAAAGGTAAATATTTACCTTTATTAGAA
  AATGGTAAATTGCTTAAAGGTTCAAATGATGTCAAAATTAATGATGCACCTGTCATGGAT
  GGTTTCAAAGGTACAAAAGAAGATGATATGATTAAGGCGTTATCTGAAATGACACCTGAA
  GTTAGACGATATATTGCCGAAGTGACATACGCCCCAAGTAAAAACAAACAAAGCAGAATT
  GAATTGTTTACGACAGATGGACTTCAAGTAATCGGTGATATTTCGACGATATCTAAGAAA
  ATGAAATATTATCCGCAGATGTCACAATCATTATCAAGGGATAGTTCGGGTAAACTAAAA
  ACACGAGGCTATATTGATTTATCAGTCGGTGCTTCATTTATCCCATACCGTGGAAACACG
  TCTAGTCAATCAGAAAGCGATAAAAATGTGACTAAATCATCTCAAGAGGAAAATCAAGCA
  AAAGAAGAATTACAAAGCGTTTTAAACAAAATTAACAAACAATCAAGTAAGAATAATTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS05845 [old locus tag: SAUSA300_1078 ]
• symbol: SAUSA300_RS05845
• description: cell division protein DivIB
• length: 439
• theoretical pI: 8.50233
• theoretical MW: 50211.6
• GRAVY: -1.03804

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 12.9)
  Metabolism Transport and binding proteins Other LPS export ABC transporter permease LptF (TIGR04407; HMM-score: 12.9)
• TheSEED: see SAUSA300_1078
• PFAM:
  POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 66.9)
  and 5 more
  POTRA; Surface antigen variable number repeat (PF07244; HMM-score: 17.5)
  no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 13.2)
  BPD_transp_1 (CL0404) YjgP_YjgQ; Predicted permease YjgP/YjgQ family (PF03739; HMM-score: 11.9)
  no clan defined DUF5316; Family of unknown function (DUF5316) (PF17247; HMM-score: 11.2)
  T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.53
  • Cellwall Score: 8.75
  • Extracellular Score: 0.7
  • Internal Helix: 1
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01083
  • TAT(Tat/SPI): 0.007453
  • LIPO(Sec/SPII): 0.004758
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446264330 NCBI
• RefSeq: WP_000342185 NCBI
• UniProt: see SAUSA300_1078

⊟Protein sequence

• MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQNKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYRYNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEKRKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLGVKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYLPLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSKNKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGASFIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications