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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04545 [old locus tag: SAUSA300_0841 ]
  • pan locus tag?: SAUPAN003037000
  • symbol: SAUSA300_RS04545
  • pan gene symbol?: ndh2b
  • synonym:
  • product: NADH dehydrogenase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04545 [old locus tag: SAUSA300_0841 ]
  • symbol: SAUSA300_RS04545
  • product: NADH dehydrogenase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 919494..920558
  • length: 1065
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAACTTAGTTTTGTTAGGCGGCGGATATGGTAATATGCGTATCATGTCACGCATT
    TTAACTACTTCTTTACCACAAGATTATACAGTCACATTAGTTGATCGTATGCCATTTCAT
    GGATTGAAACCAGAATTTTATGCTTTAGCTGCGGGCACGAAATCAGATAAAGATGTTCGT
    ATGAAATTCCCTAATCATCCACAAGTGAATACAGTTTATGGTGAAATTAACGACATAGAT
    TTAGATGCTCAAATTGTCTCAGTCGGTAATTCTAAAATTGATTATGATGAGCTAATCATT
    GGTTTAGGATGTGAAGATAAATATCATAACGTTCCAGGAGCCGAAGAATATACACATAGT
    ATTCAAACACTCTCAAAGGCTCGGGATACTTTCCATAGTATTAGTGAACTACCAGAAGGT
    GCTAAAGTCGGTATCGTTGGTGCTGGATTAAGCGGCATAGAACTTGCCAGCGAATTAAGA
    GAAAGTAGATCAGACTTGGAAATATATCTTTATGACCGTGGGCCGCGAATTTTAAGAAAT
    TTTCCAGAAAAATTAAGTAAGTATGTTGCGAAATGGTTCGCCAAAAATAATGTTACCGTT
    GTTCCAAATTCAAATATTAATAAAGTTGAACCTGGTAAAATATATAACTGTGATGAACCT
    AAAGATATTGATTTAGTTGTATGGACAGCAGGAATTCAACCTGTTGAAGTTGTTCGTAAC
    TTGCCGATTGATATAAATAGTAATGGACGCGTGATAGTTAACCAGTATCATCAAGTACCA
    ACATATCGTAACGTCTATGTAGTTGGTGATTGTGCTGATTTACCACATGCGCCAAGTGCT
    CAGTTAGCCGAAGTTCAAGGTGATCAAATTGCCGATGTGCTTAAAAAGCAATGGCTAAAT
    GAACCATTACCTGACAAAATGCCGGAACTAAAGGTACAAGGTATCGTTGGGTCATTAGGA
    GATAAGCAAGGTTTTGCATATATCATGGACCGCACTGTAACAGGACGACTCGCTTCTATT
    TTAAAATCCGGCGTACTTTGGTTATATAAATATCACAATGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1065

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS04545 [old locus tag: SAUSA300_0841 ]
  • symbol: SAUSA300_RS04545
  • description: NADH dehydrogenase family protein
  • length: 354
  • theoretical pI: 6.31904
  • theoretical MW: 39398.7
  • GRAVY: -0.272034

⊟Function[edit | edit source]

  • TIGRFAM:
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 107.7)
    and 13 more
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 70)
    oxidoreductase/SelD-related fusion protein (TIGR04369; HMM-score: 60.9)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 57.6)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 55)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 45.5)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 42.5)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 32)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 20.1)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 14.3)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 14.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 14.1)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 13)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 11.6)
  • TheSEED: see SAUSA300_0841
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 134)
    and 8 more
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 49.4)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 25.3)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 20.7)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 19.5)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 18.2)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 17.1)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 14.8)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011688
    • TAT(Tat/SPI): 0.000419
    • LIPO(Sec/SPII): 0.003593
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKPEFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGLGCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELRESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCDEPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPHAPSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVTGRLASILKSGVLWLYKYHNG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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